Effects of Red Clover Isoflavones on Growth Performance, Serum Hormone Parameters and Uterus Morphological Structure of Ovariectomized Rats

MA Yong; LI Fangfang; ZHANG Yong; ZHANG Ruiyang; ZHU Yujing

doi:10.3969/j.issn.1006-267x.2021.06.061

2021 , Vol. 33 >Issue 6: 3592 - 3600

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.06.061

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Effects of Red Clover Isoflavones on Growth Performance, Serum Hormone Parameters and Uterus Morphological Structure of Ovariectomized Rats

MA Yong ,
LI Fangfang ,
ZHANG Yong ,
ZHANG Ruiyang ,
ZHU Yujing

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College of Animal Sciences and Veterinary Sciences, Shenyang Agricultural University, Shenyang 110866, China

Received date: 2020-11-13

Online published: 2021-06-10

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Abstract

This experiment aimed to study the effects of red clover isoflavones on growth performance, serum hormone parameters and uterus morphological structure of ovariectomized rats. After one week adaption, a total of 30 specific pathogen free (SPF) female SD rats (3-month-old) weighted about (200±5) g were randomly allocated to 5 groups with 6 rats in each group, and the groups were as follows:the sham-operated group (exposed the ovary and touched the surface of ovary with forceps, then sutured the wound), control group (ovariectomized), and low, medium and high-dose red clover isoflavones groups[ovariectomized and gavaged 10, 30 and 100 mg/(kg·d) red clover isoflavones according to body weight, respectively]. The red clover isoflavones were prepared to the required concentration used 0.5% sodium carboxymethyl cellulose (CMC-Na), solution each rat gavaged 4 mL/d; the control group and sham-operated group gavaged equivalent 0.5% CMC-Na solution. The experiment lasted for 21 days. The results showed as follows:1) the average daily feed intake of rats of low, medium and high-dose red clover isoflavones groups was significantly higher than that of the control group (P<0.05), and the average daily feed intake of rats of high-dose red clover isoflavones group was significantly higher than that of the sham-operated group (P<0.05). The average daily gain of rats of control group and low, medium and high-dose red clover isoflavones groups was significantly higher than that of the sham-operated group (P<0.05), and the average daily gain of rats of low, medium and high-dose red clover isoflavones groups was significantly higher than that of the control group (P<0.05). The feed to gain ratio of rats of control group and low, medium and high-dose red clover isoflavones groups was significantly lower than that of the sham-operated group (P<0.05), and the feed to gain ratio of rats of low, medium and high-dose red clover isoflavones groups was significantly lower than that of the control group (P<0.05). 2) The serum estradiol content of rats of sham-operated group and low-dose red clover isoflavones group was significantly higher than that of the control group (P<0.05), the serum follicle stimulating hormone content of rats of high-dose red clover isoflavones group was significantly higher than that of the sham-operated group (P<0.05), and the serum luteinizing hormone content of rats of sham-operated group and low, medium and high-dose red clover isoflavones groups was significantly higher than that of the control group (P<0.05). 3) The serum growth hormone content of rats of high-dose red clover isoflavones group was significantly higher than that of the control group, sham-operated group and low-dose red clover isoflavones group (P<0.05), and the serum thyroxine content of rats of low-dose red clover isoflavones group was significantly higher than that of the other groups (P<0.05). 4) The contents of calcium and phosphorus in serum of rats of control group and low, medium and high-dose red clover isoflavones groups were significantly lower than those of the sham-operated group (P<0.05). 5) The morphological results showed that the red clover isoflavones could repair the uterine morphology of rats to different degrees. Among them, the high-dose red clover isoflavones group had a relatively normal adventitia connective tissue structure, no significant enlargement of muscle glands, thicker tube walls, smaller lumen, and the intimal columnar epithelium had changed. In conclusion, the red clover isoflavones can improve the imbalance of endogenous hormones in ovariectomized rats, and promote the rat growth by affecting the contents of reproduction and growth-related hormones in serum and the metabolism of calcium and phosphorus.

Key words： red clover isoflavones; rats; growth performance; serum hormones; uterine morphology

Cite this article

MA Yong , LI Fangfang , ZHANG Yong , ZHANG Ruiyang , ZHU Yujing . Effects of Red Clover Isoflavones on Growth Performance, Serum Hormone Parameters and Uterus Morphological Structure of Ovariectomized Rats[J]. Chinese Journal of Animal Nutrition, 2021 , 33(6) : 3592 -3600 . DOI: 10.3969/j.issn.1006-267x.2021.06.061

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