[1] YANG D,BIRAGYN A,KWAK L W,et al.Mammalian defensins in immunity:more than just microbicidal[J].Trends in Immunology,2002,23(6):291-296.
[2] SELSTED M E,OUELLETTE A J.Mammalian defensins in the antimicrobial immune response[J].Nature Immunology,2005,6(6):551-557.
[3] ZHANG G L,HIRAIWA H,YASUE H,et al.Cloning and characterization of the gene for a new epithelial β-defensin:genomic structure,chromosomal localization,and evidence for its constitutive expression[J].Journal of Biological Chemistry,1999,274(34):24031-24037.
[4] CHOI M K,LE M T,NGUYEN D T,et al.Genome-level identification,gene expression,and comparative analysis of porcine β-defensin genes[J].BMC Genetics,2012,13:98.
[5] SANG Y M,PATIL A,ZHANG G L,et al.Bioinformatic and expression analysis of novel porcine β-defensins[J].Mammalian Genome,2006,17(4):332-339.
[6] 薛现凤,韩菲菲,高彦华,等.猪β-防御素体外抗菌活性和抗氧化活性研究[J].农业生物技术学报,2012,20(11):1291-1299. XUE X F,HAN F F,GAO Y H,et al.
In vitro detections of antimicrobial and antioxidant activities of porcine β-defensins[J].Journal of Agricultural Biotechnology,2012,20(11):1291-1299.(in Chinese)
[7] ELAHI S,BUCHANAN R M,ATTAH-POKU S,et al.The host defense peptide beta-defensin 1 confers protection against
Bordetella pertussis in newborn piglets[J].Infection and Immunity,2006,74(4):2338-2352.
[8] LING Y M,CHEN J Y,GUO L B,et al.β-defensin 1 expression in HCV infected liver/liver cancer:an important role in protecting HCV progression and liver cancer development[J].Scientific Reports,2017,7:13404.
[9] SHI J,ZHANG G,WU H,et al.Porcine epithelial β-defensin 1 is expressed in the dorsal tongue at antimicrobial concentrations[J].Infection and Immunity,1999,67(6):3121-3127.
[10] LI C L,XU T T,CHEN R B,et al.Cloning,expression and characterization of antimicrobial porcine β defensin 1 in
Escherichia coli[J].Protein Expression and Purification,2013,88(1):47-53.
[11] XU J,ZHONG F,ZHANG Y H,et al.Construction of
Bacillus subtilis strain engineered for expression of porcine β-defensin-2/cecropin P1 fusion antimicrobial peptides and its growth-promoting effect and antimicrobial activity[J].Asian-Australasian Journal of Animal Sciences,2017,30(4):576-584.
[12] FAN K H,AN Y N,WANG Z R,et al.Antibacterial activity of recombinant porcine β-defensin 2[J].Pakistan Veterinary Journal,2019,39(3):411-415.
[13] YANG X,CHENG Y T,TAN M F,et al.Overexpression of porcine beta-defensin 2 enhances resistance to
Actinobacillus pleuropneumoniae infection in pigs[J].Infection and Immunity,2015,83(7):2836-2843.
[14] HUANG J,YANG X Y,WANG A T,et al.Pigs overexpressing porcine β-defensin 2 display increased resilience to
Glaesserella parasuis infection[J].Antibiotics,2020,9(12):903.
[15] HUANG C,YANG X,HUANG J,et al.Porcine beta-defensin 2 provides protection against bacterial infection by a direct bactericidal activity and alleviates inflammation via interference with the TLR4/NF-κB pathway[J].Frontiers in Immunology,2019,10:1673.
[16] HUANG J,QI Y H,WANG A T,et al.Porcine β-defensin 2 inhibits proliferation of pseudorabies virus in vitro and in transgenic mice[J].Virology Journal,2020,17:18.
[17] 齐燕华.猪β防御素2抗伪狂犬病毒作用的研究[D].硕士学位论文.武汉:华中农业大学,2016. QI Y H.Studies on the effects of procine beta defensin 2 on anti-pseudorabies virus[D].Master's Thesis.Wuhan:Huangzhong Agricultural University,2016.(in Chinese)
[18] VELDHUIZEN E J A,RIJNDERS M,CLAASSEN E A,et al.Porcine β-defensin 2 displays broad antimicrobial activity against pathogenic intestinal bacteria[J].Molecular Immunology,2008,45(2):386-394.
[19] SANG Y M,RUCHALA P,LEHRER R I,et al.Antimicrobial host defense peptides in an arteriviral infection:differential peptide expression and virus inactivation[J].Viral Immunology,2009,22(4):235-242.
[20] SU G Q,XIE K H,CHEN D W,et al.Differential expression,molecular cloning,and characterization of porcine beta defensin 114[J].Journal of Animal Science and Biotechnology,2019,10:60.
[21] XIE K H,XIE H M,SU G Q,et al.β-defensin 129 attenuates bacterial endotoxin-induced inflammation and intestinal epithelial cell apoptosis[J].Frontiers in Immunology,2019,10:2333.
[22] MUKHERJEE S,HOOPER L V.Antimicrobial defense of the intestine[J].Immunity,2015,42(1):28-39.
[23] LI Y M,XIANG Q,ZHANG Q H,et al.Overview on the recent study of antimicrobial peptides:origins,functions,relative mechanisms and application[J].Peptides,2012,37(2):207-215.
[24] ZHANG K,ZHANG H,GAO C Y,et al.Antimicrobial mechanism of pBD2 against Staphylococcus aureus[J].Molecules,2020,25(15):3513.
[25] CHEN R B,ZHANG K,ZHANG H,et al.Analysis of the antimicrobial mechanism of porcine beta defensin 2 against
E.coli by electron microscopy and differentially expressed genes[J].Scientific Reports,2018,8:14711.
[26] HUANG X X,GAO C Y,ZHAO Q J,et al.Antimicrobial characterization of site-directed mutagenesis of porcine beta defensin 2[J].PLoS One,2015,10(2):e0118170.
[27] LI C L,ZHAO Y C,SONG X Y,et al.Molecular cloning,expression and characterization of the porcine β defensin 2 in
E.coli[J].Protein and Peptide Letters,2013,20(6):715-723.
[28] GAO C Y,XU T T,ZHAO Q J,et al.Codon optimization enhances the expression of porcine beta-defensin-2 in
Escherichia coli[J].Genetics and Molecular Research,2015,14(2):4978-4988.
[29] HANCOCK R E W,HANEY E F,GILL E E.The immunology of host defence peptides:beyond antimicrobial activity[J].Nature Reviews Immunology,2016,16(5):321-334.
[30] MOOKHERJEE N,ANDERSON M A,HAAGSMAN H P,et al.Antimicrobial host defence peptides:functions and clinical potential[J].Nature Reviews Drug Discovery,2020,19(5):311-332.
[31] FRUITWALA S,EL-NACCACHE D W,CHANG T L.Multifaceted immune functions of human defensins and underlying mechanisms[J].Seminars in Cell & Developmental Biology,2019,88:163-172.
[32] 薛现凤.猪β-防御素抗菌、抗氧化功能及其对肠上皮细胞黏膜屏障功能的影响[D].硕士学位论文.杭州:浙江大学,2012. XUE X F.Antimicrobial and antioxidant functions of porcine beta-defensin and its effect on mucosal barrier function of intestinal epithelial cells[D].Master's Thesis.Hangzhou:Zhejiang University,2012.(in Chinese)
[33] HAN F F,ZHANG H W,XIA X,et al.Porcine beta-defensin 2 attenuates inflammation and mucosal lesions in dextran sodium sulfate-induced colitis[J].Journal of Immunology,2015,194(4):1882-1893.
[34] TANG Z R,XU L,SHI B S,et al.Oral administration of synthetic porcine beta-defensin-2 improves growth performance and cecal microbial flora and down-regulates the expression of intestinal Toll-like receptor-4 and inflammatory cytokines in weaned piglets challenged with enterotoxigenic
Escherichia coli[J].Animal Science Journal,2016,87(10):1258-1266.
[35] VELDHUIZEN E J A,HENDRIKS H G C J M,HOGENKAMP A,et al.Differential regulation of porcine β-defensins 1 and 2 upon Salmonella infection in the intestinal epithelial cell line IPI-2I[J].Veterinary Immunology and Immunopathology,2006,114(1/2):94-102.
[36] VELDHUIZEN E J A,KOOMEN I,ULTEE T,et al.
Salmonella serovar specific upregulation of porcine defensins 1 and 2 in a jejunal epithelial cell line[J].Veterinary Microbiology,2009,136(1/2):69-75.
[37] SU J,ZHANG Z W,HAN Y H,et al.Expression and identification of porcine β-defensin 1 in
Escherichia coli and up-regulation by
Streptococcus infection in porcine tongue
in vivo[J].International Journal of Peptide Research and Therapeutics,2012,18(2):145-152.
[38] GALINDO R C,AYOUBI P,NARANJO V,et al.Gene expression profiles of European wild boar naturally infected with
Mycobacterium bovis[J].Veterinary Immunology and Immunopathology,2009,129(1/2):119-125.
[39] RAZZUOLI E,AMADORI M,LAZZARA F,et al.
Salmonella serovar-specific interaction with jejunal epithelial cells[J].Veterinary Microbiology,2017,207:219-225.
[40] VELDHUIZEN E J A,VAN DIJK A,TERSTEEG M H G,et al.Expression of β-defensins
pBD-1 and
pBD-2 along the small intestinal tract of the pig:lack of upregulation
in vivo upon
Salmonella typhimurium infection[J].Molecular Immunology,2007,44(4):276-283.
[41] PAN Q,WANG X M,LIU T,et al.
Mycoplasma hyopneumoniae inhibits porcine beta-defensin 2 production by blocking the unfolded protein response to facilitate epithelial adhesion and infection[J].Infection and Immunity,2020,88(7):e00164-20.
[42] LIU F,ABIKO Y,NISHIMURA M,et al.Expression of inflammatory cytokines and beta-defensin 1 mRNAs in porcine epithelial rests of malassez
in vitro[J].Medical Electron Microscopy,2001,34(3):174-178.
[43] 梁钰,张坤,王梦云,等.猪β防御素研究进展[J].中国畜牧兽医,2019,46(8):2346-2353. LANG Y,ZHANG K,WANG M Y,et al.Research advance on porcine β defensins[J].China Animal Husbandry & Veterinary Medicine,2019,46(8):2346-2353.(in Chinese)
[44] WANG Q,SUN Q,QI R L,et al.Effects of
Lactobacillus plantarum on the intestinal morphology,intestinal barrier function and microbiota composition of suckling piglets[J].Journal of Animal Physiology and Animal Nutrition,2019,103(6):1908-1918.
[45] YI H B,WANG L,XIONG Y X,et al.Effects of
Lactobacillus reuteri LR1 on the growth performance,intestinal morphology,and intestinal barrier function in weaned pigs[J].Journal of Animal Science,2018,96(6):2342-2351.
[46] WANG J,JI H F,WANG S X,et al.Probiotic
Lactobacillus plantarum promotes intestinal barrier function by strengthening the epithelium and modulating gut microbiota[J].Frontiers in Microbiology,2018,9:1953.
[47] LIU H B,HOU C L,WANG G,et al.
Lactobacillus reuteri I5007 modulates intestinal host defense peptide expression in the model of IPEC-J2 cells and neonatal piglets[J].Nutrients,2017,9(6):559.
[48] ZHANG J H,DENG J,LI Y F,et al.The effect of
Lactobacillus on the expression of porcine β-defensin-2 in the digestive tract of piglets[J].Livestock Science,2011,138(1/2/3):259-265.
[49] LI X Q,ZHU Y H,ZHANG H F,et al.Risks associated with high-dose
Lactobacillus rhamnosus in an
Escherichia coli model of piglet diarrhoea:intestinal microbiota and immune imbalances[J].PLoS One,2012,7(7):e40666.
[50] ZENG X F,SUNKARA L T,JIANG W Y,et al.Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs[J].PLoS One,2013,8(8):e72922.
[51] XIONG H T,GUO B X,GAN Z S,et al.Butyrate upregulates endogenous host defense peptides to enhance disease resistance in piglets via histone deacetylase inhibition[J].Scientific Reports,2016,6:27070.
[52] DOU X J,HAN J L,SONG W T,et al.Sodium butyrate improves porcine host defense peptide expression and relieves the inflammatory response upon toll-like receptor 2 activation and histone deacetylase inhibition in porcine kidney cells[J].Oncotarget,2017,8(16):26532-26551.
[53] DOU X J,HAN J L,MA Q Y,et al.TLR2/4-mediated NF-κB pathway combined with the histone modification regulates β-defensins and interleukins expression by sodium phenyl butyrate in porcine intestinal epithelial cells[J].Food & Nutrition Research,2018,62,doi:10.29219/fnr.v62.1493.
[54] WANG J,HUANG N N,XIONG J,et al.Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase inhibition[J].International Immunopharmacology,2018,65:303-311.
[55] TIAN M,CHEN J M,WU Z H,et al.Fat encapsulation reduces diarrhea in piglets partially by repairing the intestinal barrier and improving fatty acid transport[J].Animals,2020,11(1):28.
[56] MAO X B,QI S,YU B,et al.Zn
2+ and
L-isoleucine induce the expressions of porcine β-defensins in IPEC-J2 cells[J].Molecular Biology Reports,2013,40(2):1547-1552.
[57] 任曼.支链氨基酸调控仔猪肠道防御素表达和免疫屏障功能的研究[D].博士学位论文.北京:中国农业大学,2014. REN M.Branched-chain amino acids modulate intestinal defensin expression and immune barrier function of piglets[D].Ph.D. Thesis.Beijing:China Agricultural University,2014.(in Chinese)
[58] REN M,CAI S,ZHOU T,et al.Isoleucine attenuates infection induced by
E.coli challenge through the modulation of intestinal endogenous antimicrobial peptide expression and the inhibition of the increase in plasma endotoxin and IL-6 in weaned pigs[J].Food & Function,2019,10(6):3535-3542.
[59] MAO X B,QI S,YU B,et al.Dietary
L-arginine supplementation enhances porcine β-defensins gene expression in some tissues of weaned pigs[J].Livestock Science,2012,148(1/2):103-108.
[60] 骆雪,张春勇,安清聪,等.
L-精氨酸对猪胎儿皮肤成纤维细胞免疫应激参数和β防御素基因mRNA表达的影响[J].动物营养学报,2013,25(5):1037-1044. LUO X,ZHANG C Y,AN Q C,et al.
L-arginine affects immune stressed parameters and mRNA expression of β-defensins genes in porcine fetal skin fibroblasts[J].Chinese Journal of Animal Nutrition,2013,25(5):1037-1044.(in Chinese)
[61] 陈金永.猪β-防御素基因表达特点及维生素A的调节作[D].博士学位论文.雅安:四川农业大学,2010. CHEN J Y.Porcine beta defensin gene expression and its regulation by vitamin A[D].Ph.D. Thesis.Ya'an:Sichuan Agricultural University,2010.(in Chinese)
[62] SRISOMBOON Y,POONYACHOTI S,DEACHAPUNYA C.Soy isoflavones enhance β-defensin synthesis and secretion in endometrial epithelial cells with exposure to TLR3 agonist polyinosinic-polycytidylic acid[J].American Journal of Reproductive Immunology,2017,78(4):e12694.
[63] WANG S,YANG J C,ZHANG B Y,et al.Deoxynivalenol impairs porcine intestinal host defense peptide expression in weaned piglets and IPEC-J2 cells[J].Toxins,2018,10(12):541.
[64] JIAO W,MA Q,LV X,et al.Gene expression and tissue distribution of β-defensins in Chinese Min pigs and Landrace pigs[J].Czech Journal of Animal Science,2017,62:178-183.
[65] PRUTHVIRAJ D R,VENKATACHALAPATHY R T,USHA A P,et al.Comparative expression analysis of porcine beta-defensin-1 gene between Large White Yorkshire and Ankamali pigs[J].International Journal of Livestock Research,2018,8(4):71-80.
[66] LAN J,DOU X J,LI J W,et al.
L-arginine ameliorates lipopolysaccharide-induced intestinal inflammation through inhibiting the TLR4/NF-κB and MAPK pathways and stimulating β-defensin expression
in vivo and
in vitro[J].Journal of Agricultural and Food Chemistry,2020,68(9):2648-2663.
[67] PENG Z X,WANG A R,XIE L Q,et al.Use of recombinant porcine β-defensin 2 as a medicated feed additive for weaned piglets[J].Scientific Reports,2016,6:26790.
[68] PENG Z X,WANG A R,FENG Q Y,et al.High-level expression,purification and characterisation of porcine β-defensin 2 in
Pichia pastoris and its potential as a cost-efficient growth promoter in porcine feed[J].Applied Microbiology and Biotechnology,2014,98(12):5487-5497.
[69] 胡翰,于宾宾,何启盖.猪β防御素2成熟肽在酵母中的表达[J].微生物学报,2011,51(5):704-709. HU H,YU B B,HE Q G.Expressing of porcine β-defensin-2 mature peptide in the yeast[J].Acta Microbiologica Sinica,2011,51(5):704-709.(in Chinese)
[70] 张定勇,孙蕾,杨利敏,等.猪β防御素2与猪γ干扰素在毕赤酵母中的融合表达及生物学活性比较[J].生物工程学报,2010,26(12):1652-1659. ZHANG D Y,SUN L,YANG L M,et al.Fusion expression and bioactivity comparison of porcine β-defensin-2 and porcine interferon-gamma in
Pichia pastoris[J].Chinese Journal of Biotechnology,2010,26(12):1652-1659.(in Chinese)
[71] 解民,仲飞,李秀锦, 等.重组猪β防御素2在毕赤酵母中的表达及其鉴定[J].中国兽医学报,2010,30(8):1028-1031. XIE M,ZHONG F,LI X J,et al.Expression and characterization of recombinant porcine β-defensin-2 gene in
Pichia pastoris[J].Chinese Journal of Veterinary Science,2010,30(8):1028-1031.(in Chinese)
[72] 吴雅琨,彭子欣,冯秋月,等.猪β-防御素-2转化酿酒酵母方法的比较分析[J].中国畜牧兽医,2012,39(7):138-141. WU Y K,PENG Z X,FENG Q Y,et al.Comparison analysis of two methods of porcine-β-defensin-2 gene transforming into
Saccharomyces cerevisiae[J].China Animal Husbandry & Veterinary Medicine,2012,39(7):138-141.(in Chinese)
[73] 谢飞,吴雅琨,冯秋月, 等.猪β-防御素-2基因在大肠杆菌中的融合表达及抑菌活性检测[J].中国畜牧兽医,2013,40(2):6-9. XIE F,WU Y K,FENG Q Y,et al.Soluble expression of porcine β-defensin-2 in
E.coli and identification of its antimicrobial activity[J].China Animal Husbandry & Veterinary Medicine,2013,40(2):6-9.(in Chinese)
[74] 徐海涛,马立保,何启盖,等.猪防御素基因
pBD-2在大肠杆菌中的重组和融合表达[J].中国畜牧兽医,2011,38(5):101-104. XU H T,MA L B,HE Q G,et al.Recombination and fusion expression of porcine defensin gene
pBD-2 in
E. coli[J].China Animal Husbandry & Veterinary Medicine,2011,38(5):101-104.(in Chinese)
[75] 姜丽华,卢海蓉,黄德新,等.猪β防御素1基因在毕赤酵母中的分泌表达[J].生物工程学报,2006,22(6):1036-1039. JIANG L H,LU H R,HUANG D X,et al.Expression of porcine β-defensin 1 gene in
Pichia pastoris[J].Chinese Journal of Biotechnology,2006,22(6):1036-1039.(in Chinese)
[76] 罗刚,魏泓.猪防御素基因
PBD-Ⅰ在大肠杆菌中的重组和融合表达[J].遗传,2003,25(2):146-150. LUO G,WEI H.Recombination and fusion expression of porcine defensin gene
PBD-Ⅰ in
E.coli[J].Hereditas,2003,25(2):146-150.(in Chinese)
