Effects of Synbiotic Containing Lactulose and Bacillus coagulans on Growth Performance, Nutrient Apparent Digestibilities and Blood Indexes of Weaned Piglets

ZHAO Zuyan; YANG Yunnan; LIU Riliang; ZHANG Yunlong; HU Hao; ZHENG Weijiang; YAO Wen

doi:10.3969/j.issn.1006-267x.2021.07.016

2021 , Vol. 33 >Issue 7: 3735 - 3744

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.07.016

SWINE NUTRITION AND FEED

Effects of Synbiotic Containing Lactulose and Bacillus coagulans on Growth Performance, Nutrient Apparent Digestibilities and Blood Indexes of Weaned Piglets

ZHAO Zuyan ,
YANG Yunnan ,
LIU Riliang ,
ZHANG Yunlong ,
HU Hao ,
ZHENG Weijiang ,
YAO Wen

Expand

1. Jiangsu Key Laboratory of Gastrointestinal Nutrition and Health, Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China;
2. Jiangsu Yuanshan Biological Technology Co., Ltd., Yancheng 224001, China

Received date: 2020-12-20

Online published: 2021-07-06

Supported by

Fold

Abstract

This experiment was conducted to investigate the effects of synbiotic containing lactulose and Bacillus coagulans on growth performance, nutrient apparent digestibilities and blood indexes of weaned piglets. Twenty-four healthy 27 to 28-day-old crossbred (Duroc×Landrace×Yorkshire) weaned piglets with initial body weight of (9.09±0.12) kg were randomly divided into 3 groups:control group (n=12), test group Ⅰ (n=6) and test group Ⅱ (n=6). Piglets in the control group were fed a basal diet (antibiotic-free), piglets in the test group Ⅰ were fed the basal diet+75 mg/kg chlortetracycline, and others in the test group Ⅱ were fed the basal diet+synbiotic containing lactulose and Bacillus coagulans (10 g/kg lactulose+2×10⁹ CFU/kg Bacillus coagulans). The experiment lasted for 29 days. The results showed as follows:1) on day 1, 15 and 29, there was no significant difference in the body weight among 3 groups (P>0.05). During weeks 1 to 2, there were no significant differences in the average daily gain (ADG) and average daily feed intake (ADFI) among 3 groups (P>0.05); the feed to gain ratio (F/G) of test group Ⅱ was significantly lower than that of the control group (P<0.05), and had no significant difference with test group Ⅰ (P>0.05). During weeks 3 to 4 and weeks 1 to 4, there were no significant differences in the ADG, ADFI and F/G among 3 groups (P>0.05). 2) During weeks 3 to 4, the diarrhea rate of test group Ⅰ and test group Ⅱ was significantly lower than that of the control group (P<0.05), and there was no difference between the test group Ⅰ and the test group Ⅱ (P>0.05); during weeks 1 to 2 and weeks 1 to 4, there was no difference in diarrhea rate among 3 groups (P>0.05). 3) The apparent digestibilities of gross energy and ether extract of test group Ⅰ and test group Ⅱ were significantly higher than those of the control group (P<0.05), and there was no significant difference between test group Ⅰ and test group Ⅱ (P>0.05); the crude protein apparent digestibility of test group Ⅰ was significantly higher than that of the control group (P<0.05), but there was no significant difference between test group Ⅱ and other 2 groups (P>0.05). 4) There were no significant differences in blood routine index among 3 groups (P>0.05). The serum total bilirubin content of test group Ⅰ and test group Ⅱ was significantly lower than that of the control group (P<0.05), and there was no significant difference between test group Ⅰ and test group Ⅱ (P>0.05). In summary, adding synbiotic containing lactulose and Bacillus coagulans in antibiotic-free diet can reduce the diarrhea rate of weaned piglets, increase the apparent digestibilities of gross energy and ether extract, decrease the serum total bilirubin content and the F/G.

Key words： lactulose; Bacillus coagulans; synbiotic; weaned piglets; nutrient apparent digestibilities; blood indexes

Cite this article

ZHAO Zuyan , YANG Yunnan , LIU Riliang , ZHANG Yunlong , HU Hao , ZHENG Weijiang , YAO Wen . Effects of Synbiotic Containing Lactulose and Bacillus coagulans on Growth Performance, Nutrient Apparent Digestibilities and Blood Indexes of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2021 , 33(7) : 3735 -3744 . DOI: 10.3969/j.issn.1006-267x.2021.07.016

References

[1] MOESER A J,POHL C S,RAJPUT M.Weaning stress and gastrointestinal barrier development:implications for lifelong gut health in pigs[J].Animal Nutrition,2017,3(4):313-321.

[2] CAMPBELL J M,CRENSHAW J D,POLO J.The biological stress of early weaned piglets[J].Journal of Animal Science and Biotechnology,2013,4:19.
[3] GASKINS H R,COLLIER C T,ANDERSON D B.Antibiotics as growth promotants:mode of action[J].Animal Biotechnology,2002,13(1):29-42.

[4] GADDE U,KIM W H,OH S T,et al.Alternatives to antibiotics for maximizing growth performance and feed efficiency in poultry:a review[J].Animal Health Research Reviews,2017,18(1):26-45.

[5] 中华人民共和国农业农村部.中华人民共和国农业农村部公告第194号[J].浙江畜牧兽医,2020,45(1):28. Ministry of Agriculture and Rural Affairs of the People's Republic of China.Announcement No.194 of the Ministry of Agriculture and Rural Affairs of the People's Republic of China[J].Zhejiang Journal Animal Science and Veterinary Medicine,2020,45(1):28.(in Chinese)
[6] MENG Q W,YAN L,AO X,et al.Influence of probiotics in different energy and nutrient density diets on growth performance,nutrient digestibility,meat quality,and blood characteristics in growing-finishing pigs[J].Journal of Animal Science,2010,88(10):3320-3326.

[7] 苗华彪,李正洲,赵元昊,等.凝结芽孢杆菌在生猪养殖中的应用及其作用机理的研究进展[J].中国畜牧兽医,2020,47(8):2433-2444. MIAO H B,LI Z Z,ZHAO Y H,et al.Advance on the application and mechanism of Bacillus coagulans in pig breeding[J].China Animal Husbandry and Veterinary Medicine,2020,47(8):2433-2444.(in Chinese)
[8] ZHOU Y H,ZENG Z H,XU Y B,et al.Application of Bacillus coagulans in animal husbandry and its underlying mechanisms[J].Animals,2020,10(3):454.
[9] WU T,ZHANG Y,LV Y,et al.Beneficial impact and molecular mechanism of Bacillus coagulans on piglets' intestine[J].International Journal of Molecular Sciences,2018,19(7):2084.
[10] JÄGER R,PURPURA M,FARMER S,et al.Probiotic Bacillus coagulans GBI-30,6086 improves protein absorption and utilization[J].Probiotics and Antimicrobial Proteins,2018,10(4):611-615.

[11] BAMI M K,AFSHARMANESH M,EBRAHIMNEJAD H.Effect of dietary Bacillus coagulans and different forms of zinc on performance,intestinal microbiota,carcass and meat quality of broiler chickens[J].Probiotics and Antimicrobial Proteins,2020,12(2):461-472.

[12] BOMKO T V,NOSALSKAYA T N,KABLUCHKO T V,et al.Immunotropic aspect of the Bacillus coagulans probiotic action[J].Journal of Pharmacy and Pharmacology,2017,69(8):1033-1040.

[13] KIM B R,CHO K J,KIM D,et al.Evaluation of synbiotics as gut health improvement agents against Shiga toxin-producing Escherichia coli isolated from the pig[J].Journal of Animal Science and Technology,2019,61(2):55-60.

[14] RYCROFT C E,JONES M R,GIBSON G R,et al.A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides[J].Journal of Applied Microbiology,2001,91(5):878-887.

[15] WATSON D,MOTHERWAY M O,SCHOTERMAN M H C,et al.Selective carbohydrate utilization by Lactobacilli and Bifidobacteria[J].Journal of Applied Microbiology,2013,114(4):1132-1146.

[16] HOSSAIN M M,PARK J W,KIM I H.δ-aminolevulinic acid,and lactulose supplements in weaned piglets diet:effects on performance,fecal microbiota,and in-vitro noxious gas emissions[J].Livestock Science,2016,183:84-91.
[17] SARAO L K,ARORA M.Probiotics,prebiotics,and microencapsulation:a review[J].Critical Reviews in Food Science and Nutrition,2017,57(2):344-371.

[18] GOURBEYRE P,DENERY S,BODINIER M.Probiotics,prebiotics,and synbiotics:impact on the gut immune system and allergic reactions[J].Journal of Leukocyte Biology,2011,89(5):685-695.

[19] WALSH A M,SWEENEY T,O'SHEA C J,et al.Effect of supplementing different ratios of laminarin and fucoidan in the diet of the weanling piglet on performance,nutrient digestibility,and fecal scoring[J].Journal of Animal Science,2012,90(Suppl.4):215-217.
[20] 李方方,杨晶晶,张瑞阳,等.植物精油对断奶仔猪生长性能、血清生化指标及养分表观消率的影响[J].动物营养学报,2019,31(3):1428-1433. LI F F,YANG J J,ZHANG R Y,et al.Effects of plant essential oils on growth performance,serum biochemical indexes and nutrient apparent digestibility of weaning piglets[J].Chinese Journal of Animal Nutrition,2019,31(3):1428-1433.(in Chinese)
[21] 邓雪娟,刘国华,蔡辉益,等.分光光度计法测定家禽饲料和食糜中二氧化钛[J].饲料工业,2008,29(2):57-58. DEND X J,LIU G H,CAI H Y.Determination of titanium dioxide in poultry feed and chyme by spectrophotometer[J].Feed Industry,2008,29(2):57-58.(in Chinese)
[22] DUARTE M E,ZHOU F X,DUTRA W M,Jr,et al.Dietary supplementation of xylanase and protease on growth performance,digesta viscosity,nutrient digestibility,immune and oxidative stress status,and gut health of newly weaned pigs[J].Animal Nutrition,2019,5(4):351-358.

[23] PU J N,CHEN D W,TIAN G,et al.Effects of benzoic acid,Bacillus coagulans and oregano oil combined supplementation on growth performance,immune status and intestinal barrier integrity of weaned piglets[J].Animal Nutrition,2020,6(2):152-159.

[24] 黄遵锡,程志斌.饲粮不同添加量的凝结芽孢杆菌对仔猪生长性能及肠道健康的影响[J].饲料工业,2020,41(15):1-7. HUANG Z X,HE Z B.Effects of dietary supplementation of probiotic Bacillus coagulans at various dosages on the growth performance and gut health in weanling piglets[J].Feed Industry,2020,41(15):1-7. (in Chinese)
[25] GUERRA-ORDAZ A A,MOLIST F,HERMES R G,et al.Effect of inclusion of lactulose and Lactobacillus plantarum on the intestinal environment and performance of piglets at weaning[J].Animal Feed Science and Technology,2013,185(3/4):160-168.
[26] 陈旭东.果寡糖和芽孢杆菌对断奶仔猪生产性能的影响及其机理[D].硕士学位论文.北京:中国农业大学,2004. CHEN X D.Study of FOS and Bacillus on growth performance and machanism in weanling pigs[D].Master's Thesis.Beijing:China Agricultural University,2004.(in Chinese)
[27] XIAO D F,WANG Y F,LIU G,et al.Effects of chitosan on intestinal inflammation in weaned pigs challenged by enterotoxigenic Escherichia coli[J].PLoS One,2014,9(8):e104192.
[28] HYRONIMUS B,LE MARREC C,URDACI M C.Coagulin,a bacteriocin-like inhibitory substance produced by Bacillus coagulans I4[J].Journal of Applied Microbiology,1998,85(1):42-50.

[29] NYANGALE E P,FARMER S,KELLER D,et al.Effect of prebiotics on the fecal microbiota of elderly volunteers after dietary supplementation of Bacillus coagulans GBI-30,6086[J].Anaerobe,2014,30:75-81.
[30] MAATHUIS A J,KELLER D,FARMER S.Survival and metabolic activity of the Ganeden BC³⁰ strain of Bacillus coagulans in a dynamic in vitro model of the stomach and small intestine[J].Beneficial Microbes,2010,1(1):31-36.

[31] PARK J,KOTANI T,KONNO T,et al.Promotion of intestinal epithelial cell turnover by commensal bacteria:role of short-chain fatty acids[J].PLoS One,2016,11(5):e0156334.
[32] ZHANG Z,ZHAO J T,TIAN C Y,et al.Targeting the gut microbiota to investigate the mechanism of lactulose in negating the effects of a high-salt diet on hypertension[J].Molecular Nutrtion and Food Research,2019,63(11):1800941.
[33] 孙梅,张一平,张宪中,等.芽孢杆菌制剂对断奶仔猪血液和免疫指标的影响[J].中国畜牧兽医,2015,42(11):2991-2998. SUN M,ZHANG Y P,ZHANG X Z,et al.Effects of Bacillus preparation on the blood and immunologic indices in weaned piglets[J].China Animal Husbandry and Veterinary Medicine,2015,42(11):2991-2998.(in Chinese)
[34] ZHAO P Y,JUNG J H,KIM I H.Effect of mannan oligosaccharides and fructan on growth performance,nutrient digestibility,blood profile,and diarrhea score in weanling pigs[J].Journal of Animal Science,2012,90(3):833-839.

[35] AGRAWAL S,DHIMAN R K,LIMDI J K.Evaluation of abnormal liver function tests[J].Postgraduate Medical Journal,2016,92(1086):223-234.

[36] 张青,计徐,姚文,等.乳果糖和富氢水对采食镰刀菌污染玉米的仔猪血液指标和肝脾形态的影响[J].畜牧与兽医,2018,50(5):56-64. ZHANG Q,JI X,YAO W,et al.Effects of lactulose and hydrogen-rich water onblood indices and histomorphology of liver and spleen in piglets fed with fusarium mycotoxin contaminated corn[J].Animal Husbandry and Veterinary Medicine,2018,50(5):56-64.(in Chinese)
[37] JAFARPOUR D,SHEKARFOROUSH S S,GHAISARI H R,et al.Protective effects of synbiotic diets of Bacillus coagulans,Lactobacillus plantarum and inulin against acute cadmium toxicity in rats[J].BMC Complementary and Alternative Medicine,2017,17:291.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References