Effects of Ornithine-&alpha;-Ketoglutarate on Growth Performance, Organ Indexes, Intestinal Morphology and Serum Biochemical Indexes of Piglets with Chronic Oxidative Stress Caused by D-Galactose

WANG Peng; LI Yuying; TIAN Junquan; SU Wenxuan; YIN Jie; YIN Yulong; YAO Kang

doi:10.3969/j.issn.1006-267x.2021.07.018

2021 , Vol. 33 >Issue 7: 3759 - 3767

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.07.018

SWINE NUTRITION AND FEED

Effects of Ornithine-α-Ketoglutarate on Growth Performance, Organ Indexes, Intestinal Morphology and Serum Biochemical Indexes of Piglets with Chronic Oxidative Stress Caused by D-Galactose

WANG Peng ,
LI Yuying ,
TIAN Junquan ,
SU Wenxuan ,
YIN Jie ,
YIN Yulong ,
YAO Kang

Expand

1. Hunan Provincial Engineering Research Center for Healthy Breeding of Livestock and Poultry, Key Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410025, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2020-12-29

Online published: 2021-07-06

Supported by

Fold

Abstract

The aim of this study was to investigate the effects of ornithine-α-ketoglutarate (OKG) on growth performance, organ indexes, intestinal morphology and serum biochemical indexes of piglets with chronic oxidative stress caused by D-galactose. A total of 40 castrated and healthy weaned piglets (Landrace×Yorkshire) with the average body weight of (7.68±0.56) kg were randomly assigned to five groups with 8 repetition per group and 1 piglet per repetition:control group (basal diet), model group (basal diet+5 g/kg BW D-galactose), SOKG group (basal diet+5 g/kg BW D-galactose+0.5% OKG), MOKG group (basal diet+5 g/kg BW D-galactose+1.0% OKG) and LOKG group (basal diet+5 g/kg BW D-galactose+2.0% OKG). The experimental lasted for 28 days. At the end of the experiment, the piglet's growth performance, organ indexes, intestinal morphology and serum biochemical indexes were determined. The results showed as follows:1) the final body weight, average daily gain (ADG) and average daily feed intake (ADFI) of the model group were significantly lower, and the feed/gain (F/G) was significantly higher than those of the control group (P<0.05); the final body weight and ADG of the SOKG group were significantly higher, and the F/G was significantly lower than that of the model group (P<0.05); the F/G of the MOKG group was significantly lower than that of the model group (P<0.05). 2) The spleen index and kidney index of the MOKG group were significantly higher than those of the control group and the model group (P<0.05). 3) The jejunal villus height of the SOKG group was significantly higher than that of the model group (P<0.05); the ileal crypt depth in the MOKG group and LOKG group was lower than that of the model group (P>0.05). 4) Serum total protein (TP) content of the MOKG group was significantly increased compared with the control group (P<0.05); serum alkaline phosphatase (ALP) activity of the model group was significantly decreased compared with the control group (P<0.05), while serum ALP activity of the SOKG group was significantly increased compared with the model group (P<0.05); serum immunoglobulin G (IgG) content of the MOKG group was significantly increased compared with the control group (P<0.05); serum immunoglobulin M (IgM) content of the MOKG group and LOKG group was significantly increased compared with the control group and model group (P<0.05). Collectively, these results indicate that OKG can alleviate the growth-suppression, promote the spleen and kidney growth, improve the morphological structure of intestinal mucosa, increase the serum TP, IgG, IgM contents and ALP activity, and alleviate the chronic oxidative stress of piglets caused by D-galactose.

Key words： ornithine-α-ketoglutarate; D-galactose; chronic oxidative stress; piglets; growth performance; organ indexes; intestinal morphology; serum biochemical indexes

Cite this article

WANG Peng , LI Yuying , TIAN Junquan , SU Wenxuan , YIN Jie , YIN Yulong , YAO Kang . Effects of Ornithine-α-Ketoglutarate on Growth Performance, Organ Indexes, Intestinal Morphology and Serum Biochemical Indexes of Piglets with Chronic Oxidative Stress Caused by D-Galactose[J]. Chinese Journal of Animal Nutrition, 2021 , 33(7) : 3759 -3767 . DOI: 10.3969/j.issn.1006-267x.2021.07.018

References

[1] BETTERIDGE D J.What is oxidative stress?[J].Metabolism:Clinical and Experimental,2000,49(2):3-8.

[2] WANG W W,WU Z L,LIN G,et al.Glycine stimulates protein synthesis and inhibits oxidative stress in pig small intestinal epithelial cells[J].The Journal of Nutrition,2014,144(10):1540-1548.

[3] PARK S,KIM C S,LEE J,et al.Effect of regular exercise on the histochemical changes of D-galactose-induced oxidative renal injury in high-fat diet-fed rats[J].Acta Histochemica et Cytochemica,2013,46(4):111-119.

[4] LIU C M,MA J Q,LOU Y.Chronic administration of troxerutin protects mouse kidney against D-galactose-induced oxidative DNA damage[J].Food and Chemical Toxicology,2010,48(10):2809-2817.

[5] ZHONG J J,WANG F,WANG Z F,et al.Aloin attenuates cognitive impairment and inflammation induced by D-galactose via down-regulating ERK,p38 and NF-κB signaling pathway[J].International Immunopharmacology,2019,72:48-54.
[6] FENG Y,YU Y H,WANG S T,et al.Chlorogenic acid protects D-galactose-induced liver and kidney injury via antioxidation and anti-inflammation effects in mice[J].Pharmaceutical Biology,2016,54(6):1027-1034.

[7] ZHANG J M,LIN W W,WU R J,et al.Mechanisms of the active components from Korean pine nut preventing and treating D-galactose-induced aging rats[J].Biomedicine and Pharmacotherapy,2018,103:680-690.
[8] SEGAUD F,LARDEUX B,ALEXANDRE-GOUABAU M C,et al.Pretreatment of starved rats with ornithine α-ketoglutarate:effects on hepatic mRNA levels and plasma concentrations of three liver-secreted proteins[J].Nutrition,2005,21(6):732-739.

[9] LOÏ C,HAMANI D,MOINARD C,et al.Does the ornithine-α-ketoglutarate ratio influence ornithine α-ketoglutarate metabolism in healthy rats?[J].Metabolism:Clinical and Experimental,2007,56(1):105-114.

[10] TATARA M R, ŚLIWA E,KRUPSKI W,et al.Ornithine alpha-ketoglutarate increases mineralization and mechanical properties of tibia in turkeys[J].Bone,2006,39(1):100-105.

[11] JEEVANANDAM M,HOLADAY N J,PETERSEN S R.Ornithine-α-ketoglutarate (OKG) supplementation is more effective than its component salts in traumatized rats[J].Journal of Nutrition,1996,126(9):2141-2150.

[12] SCHUSTER H,BLANC M C,GENTHON C,et al.Does dietary ornithine α-ketoglutarate supplementation protect the liver against ischemia-reperfusion injury?[J].Clinical Nutrition,2005,24(3):375-384.

[13] GONÇALVES E S G,RABELO C M,DO PRADO NETO A X,et al.Effect of short-term ornithine alpha-ketoglutarate pretreatment on intestinal ischemia-reperfusion in rats[J].Acta Cirurgica Brasileira,2011,26(Suppl.1):2-7.
[14] PERNET P,COUDRAY-LUCAS C,SCHNEID C,et al.Dose dependency of the effect of ornithine α-ketoglutarate on tissue glutamine concentrations and hypercatabolic response in endotoxaemic rats[J].British Journal of Nutrition,2004,92(4):627-634.

[15] SCHNEID C,DE BANDT J P,CYNOBER L,et al.In vivo induction of insulin secretion by ornithine α-ketoglutarate:involvement of nitric oxide and glutamine[J].Metabolism:Clinical and Experimental,2003,52(3):344-350.

[16] LI Y Y,WANG P,YIN J,et al.Effects of ornithine α-ketoglutarate on growth performance and gut microbiota in a chronic oxidative stress pig model induced by D-galactose[J].Food & Function,2020,11(1):472-482.

[17] MOINARD C,CALDEFIE F,WALRAND S,et al.Effects of ornithine 2-oxoglutarate on neutrophils in stressed rats:evidence for the involvement of nitric oxide and polyamines[J].Clinical Science,2002,102(3):287-295.

[18] ALBAUGH V L,MUKHERJEE K,BARBUL A.Proline precursors and collagen synthesis:biochemical challenges of nutrient supplementation and wound healing[J].Journal of Nutrition,2017,147(11):2011-2017.
[19] LE BRICON T,COUDRAY-LUCAS C,LIORET N,et al.Ornithine α-ketoglutarate metabolism after enteral administration in burn patients:bolus compared with continuous infusion[J].American Journal of Clinical Nutrition,1997,65(2):512-518.

[20] LE BRICON T,CYNOBER L,BARACOS V E.Ornithine α-ketoglutarate limits muscle protein breakdown without stimulating tumor growth in rats bearing Yoshida ascites hepatoma[J].Metabolism:Clinical and Experimental,1994,43(7):899-905.

[21] ZHANG H Y,LI Y Y,CUI C X,et al.Modulation of gut microbiota by dietary supplementation with tuna oil and algae oil alleviates the effects of D-galactose-induced ageing[J].Applied Microbiology and Biotechnology,2018,102(6):2791-2801.

[22] 王黎琦,汪攀,曹珺,等.约氏乳杆菌对断奶仔猪生产性能、血液学指标、脏器指数和组织病理学的影响[J].中国畜牧杂志,2016,52(21):76-80. WANG L Q,WANG P,CAO J,et al.Effects of Lactobacillus johnsonii on growth performance,hematology indices,organ indices and tissues histopathology in weaned pigs[J].Chinese Journal of Animal Science,2016,52(21):76-80.(in Chinese)
[23] RUAN Q W,LIU F,GAO Z J,et al.The anti-inflamm-aging and hepatoprotective effects of huperzine A in D-galactose-treated rats[J].Mechanisms of Ageing and Development,2013,134(3/4):89-97.
[24] WANG W W,QIAO S Y,LI D F.Amino acids and gut function[J].Amino Acids,2009,37(1):105-110.

[25] YAO K,YIN Y L,LI X L,et al.Alpha-ketoglutarate inhibits glutamine degradation and enhances protein synthesis in intestinal porcine epithelial cells[J].Amino Acids,2012,42(6):2491-2500.

[26] 李梦云,欧阳五庆,吴小利,等.激动素对衰老大鼠免疫功能及其脾淋巴细胞体外增殖的影响[J].生理学报,2014,66(5):605-611. LI M Y,OUYANG W Q,WU X L,et al.Effect of kinetin on immunity and splenic lymphocyte proliferation in vitro in D-galactose-induced aging rats[J].Acta Physiologica Sinica,2014(5):605-611.(in Chinese)

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References