POULTRY NUTRITION AND FEED

Effects of Gracilaria lemaneiformis Polysaccharides on Growth Performance, Antioxidant Capacity and Immune Function of Broilers

  • LI Wenjia ,
  • XU Bin ,
  • WANG Linyi ,
  • FU Chen ,
  • WANG Gaili ,
  • MA Huihui ,
  • SUN Quanyou ,
  • DENG Wen ,
  • BAI Jie ,
  • XI Yanyan ,
  • WEI Fengxian ,
  • LI Shaoyu
Expand
  • Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

Received date: 2020-12-09

  Online published: 2021-07-06

Supported by

 

Abstract

This study was conducted to evaluate the effects of dietary Gracilaria lemaneiformis polysaccharides (GLP) on growth performance, serum antioxidant capacity and immune function of broilers. Two hundred and eighty-eight 1-day-old healthy Arbor Acres (AA) broilers with similar body weight were randomly divided into 3 groups with 6 replicates per group and 16 broilers per replicate (males and females in half). The broilers in control (CON) group was fed a basal diet, and those in GLP and antibiotic (ANTI) groups were fed the basal diet supplemented with 0.1% GLP and 10 mg/kg ANTI, respectively. The experiment lasted for 42 days consisting of 2 stages of 1 to 21 days of age and 22 to 42 days of age. The result showed as follows:1) final body weight and average daily gain (ADG) of broilers in GLP and ANTI groups were significantly higher than those in CON group during 22 to 42 days of age (P<0.05), and the ratio of feed to gain (F/G) was significantly lower than that in CON group during 1 to 21 days of age and 22 to 42 days of age (P<0.05). 2) Serum malondialdehyde (MDA) content of broilers in GLP group was significantly lower than that in CON group at 21 and 42 days of age (P<0.05), and serum superoxide dismutase (SOD) activity at 42 days of age and serum glutathione peroxidase (GSH-Px) activity at 21 and 42 days of age were significantly higher than those in CON and ANTI groups (P<0.05). 3) Serum immunoglobulin G (IgG) content of broilers in GLP group was significantly higher than that in CON and ANTI groups at 42 days of age (P<0.05), and serum immunoglobulin M (IgM) content in ANTI group was significantly lower than that in the other 2 groups at 21 and 42 days of age (P<0.05). 4) Serum tumor necrosis factor-α (TNF-α) content of broilers in GLP group was significantly lower than that in the other 2 groups at 21 days of age (P<0.05), and was significantly lower than that in ANTI group at 42 days of age (P<0.05). Serum interleukin-1β (IL-1β) content of broilers in GLP and ANTI groups was significantly lower than that in CON group at 21 and 42 days of age (P<0.05). 5) The mRNA relative expression level of IL-1β at 21 days of age and TNF-α at 42 days of age in intestinal mucosa of broilers in GLP and ANTI groups were significantly lower than those in CON group (P<0.05), and the mRNA relative expression level of IL-1β in intestinal mucosa in GLP group was significantly lower than that in the other 2 groups at 42 days of age (P<0.05). In conclusion, dietary 0.1% GLP can improve growth performance and feed utilization, enhance antioxidant capacity and immune function, and alleviate intestinal inflammatory reaction of broilers to a certain extent.

Cite this article

LI Wenjia , XU Bin , WANG Linyi , FU Chen , WANG Gaili , MA Huihui , SUN Quanyou , DENG Wen , BAI Jie , XI Yanyan , WEI Fengxian , LI Shaoyu . Effects of Gracilaria lemaneiformis Polysaccharides on Growth Performance, Antioxidant Capacity and Immune Function of Broilers[J]. Chinese Journal of Animal Nutrition, 2021 , 33(7) : 3778 -3789 . DOI: 10.3969/j.issn.1006-267x.2021.07.020

References

[1] HONG P Y,LI X Z,YANG X F,et al.Monitoring airborne biotic contaminants in the indoor environment of pig and poultry confinement buildings[J].Environmental Microbiology,2012,14(6):1420-1431.  
[2] BEN W W,WANG J,PAN X,et al.Dissemination of antibiotic resistance genes and their potential removal by on-farm treatment processes in nine swine feedlots in Shandong province,China[J].Chemosphere,2017,167:262-268.
[3] DIBAISE J K,ZHANG H S,CROWELL M D,et al.Gut microbiota and its possible relationship with obesity[J].Mayo Clinic Proceedings,2008,83(4):460-469.  
[4] WU N,QIAO M,ZHANG B,et al.Abundance and diversity of tetracycline resistance genes in soils adjacent to representative swine feedlots in China[J].Environmental Science & Technology,2010,44(18):6933-6939.  
[5] PAL C,BENGTSSON-PALME J,KRISTIANSSON E,et al.The structure and diversity of human,animal and environmental resistomes[J].Microbiome,2016,4(1):54.
[6] 刘菲,许霞,屠博文,等.某集约化肉鸡饲养场PM2.5中抗生素抗性基因的分布特征[J].环境科学,2019,40(2):567-572. LIU F,XYV X,TU B W,et al.Distribution characteristics of antibiotic resistance genes in PM2.5 of a concentrated broiler feeding operation[J].Environmental Science,2019,40(2):567-572.(in Chinese)
[7] HE J Z,XU Y Y,CHEN H B,et al.Extraction,structural characterization,and potential antioxidant activity of the polysaccharides from four seaweeds[J].International Journal of Molecular Sciences,2016,17(12):1988.
[8] WU S J,LU M S,WANG S J.Amylase-assisted extraction and antioxidant activity of polysaccharides from Gracilaria lemaneiformis[J].3 Biotech,2017,7(1):38.
[9] FAN Y L,WANG W H,SONG W,et al.Partial characterization and anti-tumor activity of an acidic polysaccharide from Gracilaria lemaneiformis[J].Carbohydrate Polymers,2012,88(4):1313-1318.  
[10] YAN J K,PEI J J,MA H L,et al.Advances in antitumor polysaccharides from Phellinus sensu lato:production,isolation,structure,antitumor activity,and mechanisms[J].Critical Reviews in Food Science and Nutrition,2017,57(6):1256-1269.  
[11] REN Y L,ZHENG G Q,YOU L J,et al.Structural characterization and macrophage immunomodulatory activity of a polysaccharide isolated from Gracilaria lemaneiformis[J].Journal of Functional Foods,2017,33:286-296.
[12] LIAO X B,YANG L W,CHEN M Z,et al.The hypoglycemic effect of a polysaccharide (GLP) from Gracilaria lemaneiformis and its degradation products in diabetic mice[J].Food & Function,2015,6(8):2542-2549.  
[13] AI C Q,DUAN M M,MA N,et al.Sulfated polysaccharides from pacific abalone reduce diet-induced obesity by modulating the gut microbiota[J].Journal of Functional Foods,2018,47:211-219.
[14] 郭晓波,邹田德,杨晋,等.海藻多糖对仔猪小肠上皮细胞紧密连接蛋白及核转录因子-κB信号通路相关基因表达的影响[J].动物营养学报,2020,32(6):2816-2823. GUO X B,ZHOU T D,YANG J,et al.Effects of seaweed polysaccharides on expression of tight junction protein and related genes in nuclear transcription factor-κB signaling pathway in porcine epithelial cells[J].Chinese Journal of Animal Nutrition,2020,32(6):2816-2823.(in Chinese)
[15] 萨仁娜.海带岩藻聚糖分级纯化及对肉仔鸡巨噬细胞免疫调节的研究[D].博士学位论文.北京:中国农业科学院,2007. SA R N.Isolation and purification of fucoidan from laminaria japonica and its immunomodulatory activities on broiler macrophage[D].Ph.D.Thesis.Beijing:Chinese Academy of Agricultural Sciences,2008.(in Chinese)
[16] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△Ct method[J].Methods,2001,25(4):402-408.  
[17] 胡庭俊,刘姗姗,韦现色,等.马尾藻多糖纳米脂质体对鸡生长发育及免疫功能的影响[J].饲料工业,2012,33(3):32-35. HU J T,LIU S S,WEI X S,et al.Effects of Sargassum nano-polysaccharide liposome on growth and immune function of chickens[J].Feed Industry,2012,33(3):32-35.(in Chinese)
[18] 杨晋.海藻多糖替代抗生素对断奶仔猪生长性能和肠道屏障功能的影响[D].硕士学位论文.南昌:江西农业大学,2019. YANG J.Effects of seaweed polysaccharide substitute for antibiotics on growth performance and intestinal barrier function of weaned piglets[D].Master's Thesis.Nanchang:Jiangxi Agricultural University,2019.(in Chinese)
[19] HEIM G,WALSH A M,SWEENEY T,et al.Effect of seaweed-derived laminarin and fucoidan and zinc oxide on gut morphology,nutrient transporters,nutrient digestibility,growth performance and selected microbial populations in weaned pigs[J].British Journal of Nutrition,2014,111(9):1577-1585.  
[20] MCALPINE P,O'SHEA C J,VARLEY P F,et al.The effect of seaweed extract as an alternative to zinc oxide diets on growth performance,nutrient digestibility,and fecal score of weaned piglets[J].Journal of Animal Science,2012,90(Suppl.4):224-226.
[21] 孙福昱,南雪梅,唐志文,等.海藻在畜牧生产中的应用研究进展[J].中国畜牧兽医,2019,46(1):157-165. SUN F Y,NAN X M,TANG Z W,et al.Research progress on seaweed application in animal husbandry production[J].China Animal Husbandry and Veterinary Medicine,2019,46(1):157-165.(in Chinese)
[22] MACFARLANE G T,HAY S,MACFARLANE S,et al.Effect of different carbohydrates on growth,polysaccharidase and glycosidase production by Bacteroides ovatus,in batch and continuous culture[J].Journal of Applied Bacteriology,1990,68(2):179-187.  
[23] LEONARD S C,SWEENEY T BAHAR B,et al.Effect of maternal seaweed extract supplementation on suckling piglet growth,humoral immunity,selected microflora,and immune response after an ex vivo lipopolysaccharide challenge[J].Journal of Animal Science,2012,90(2):505-514.  
[24] WALTER J.Ecological role of lactobacilli in the gastrointestinal tract:implications for fundamental and biomedical research[J].Applied and Environmental Microbiology,2008,74(16):4985-4996.  
[25] 万顺康,左绍远,张翠香.螺旋藻多糖对肉仔鸡生长性能、免疫功能及生化指标的影响[J].饲料研究,2013(9):70-73. WAN S K,ZUO S Y,ZHANG C X.Effects of Spirulina polysaccharide on growth performance,immune function and biochemical indexes of broilers[J].Feed Research,2013(9):70-73.(in Chinese)
[26] 阎桂玲,呙于明.褐藻酸寡糖对肉鸡生长及免疫机能的影响[C]//动物营养与饲料研究:第六次全国饲料营养学术研讨会论文集.杨凌:中国畜牧兽医学会动物营养学分会,2010:1. YAN G L,GUO Y M.Effect of alginate-oligosaccharides on growth performance and immunity in broilers[C]//Animal nutrition and feed research:Proceedings of the sixth national symposium on feed nutrition.Yangling:Animal Nutrition Branch of Chinese Society of Animal Husbandry and Veterinary Medicine,2010:1.(in Chinese)
[27] 任颖朗,季德胜,张云林,等.龙须菜多糖的提取及其免疫调节活性研究[J].现代食品科技,2017,33(10):45-51. REN Y L,JI D S,ZHANG Y L,et al.Extraction of polysaccharides from Gracilaria lemaneiformis and their immunoregulatory activity[J].Modern Food Science and Technology,2017,33(10):45-51.(in Chinese)
[28] 王烨,胡中泽.海藻多糖对肉杂鸡免疫功能的影响[J].安徽农学通报,2010,16(17):59-62,70. WANG Y,HU Z Z.Effects of algae polysaccharides on the immune function of crossing chickens[J].Anhui Agricultural Science Bulletin,2010(17):59-62.(in Chinese)
[29] LI W J,WEI F X,XU B,et al.Effect of stocking density and alpha-lipoic acid on the growth performance,physiological and oxidative stress and immune response of broilers[J].Asian-Australasian Journal of Animal Sciences,2019,32(12):1914-1922.  
[30] 宫春宇,张慧君,余世峰,等.龙须菜超滤多糖体外抗氧化活性研究[J].食品工业,2012,33(7):92-94. GONG C Y,ZHANG H J,YU S F,et al.Antioxidant activity in vitro of polysaccharides from Gracilaria lemaneiformis[J].The Food Industry,2012,33(7):92-94. (in Chinese)
[31] 杨文鸽,谢果凰,徐大伦,等.龙须菜多糖的降解及其降解产物的抗氧化活性[J].水产学报,2009,33(2):342-347. YANG W G,XIE G H,XV D L,et al.Degradation of Gracilaria lemaneiform is polysaccharide and antioxidation of its degraded products[J].Journal of Fisheries of China,2009,33(2):342-347.(in Chinese)
[32] GUO D,YU K,SUN X Y,et al.Structural characterization and repair mechanism of Gracilaria lemaneiformis sulfated polysaccharides of different molecular weights on damaged renal epithelial cells[J].Oxidative Medicine and Cellular Longevity,2018,2018:1-15.
[33] 陈美珍,余杰,廖灶辉,等.龙须菜多糖抑瘤活性及对荷瘤小鼠抗氧化作用的研究[J].中国海洋药物,2008,27(2):46-49. CHEN M Z,YU J,LIAO Z H,et al.Study on the antitumor activity and antioxidation of polysaccharide from Gracilaria lemaneiformis in sarcoma180 bearing mice[J].Chinese Journal of Marine Drugs,2008,27(2):46-49.(in Chinese)
[34] 王晓梅,张忠山,周俊波,等.龙须菜多糖的提取纯化及生物活性研究进展[J].海洋渔业,2020,42(1):120-128. WANG X M,ZHANG Z S,ZHOU J B,et al.Research progress on extraction,purification and physiological activity of polysaccharides from Gracilaria lemaneiformis[J].Marine Fisheries,2020,42(1):120-128.(in Chinese)
[35] 石达友,刘汉儒,卓曲,等.中药提取物对鸡免疫器官发育和新城疫抗体水平的影响[J].中兽医学杂志,2004(2):4-5. SHI D Y,LIU H R,ZHUO Q,et al.Effects of Chinese herbal extracts on the development of immune organs and the level of antibodies against Newcastle disease in chickens[J].Chinese Journal of Traditional Veterinary Science,2004(2):4-5.(in Chinese)
[36] 于敬沂.几种海藻多糖的提取及其抗氧化、抗病毒(TMV)活性研究[D].硕士学位论文.福州:福建农林大学,2005. YU J Q.Studies on the extraction and the activities of antioxidation and anti-virus(TMV) of polysaccharides from several sea algaes[D].Master's Thesis.Fuzhou:Fujian Agriculture and Forestry University,2005.(in Chinese)
[37] 杨华,庄陈丰.响应面法优化微波辅助提取龙须菜多糖工艺及其抗氧化活性研究[J].食品科学,2011,32(20):79-83. YANG H,ZHUANG C F.Gracilaria lamaneiformis polysaccharides:optimization of microwave-assisted extraction by response surface methodology and antioxidant properties[J].Food Science,2011,32(20):79-83.(in Chinese)
[38] SOUZA B W S,CERQUEIRA M A,BOURBON A I,et al.Chemical characterization and antioxidant activity of sulfated polysaccharide from the red seaweed Gracilaria birdiae[J].Food Hydrocolloids.2012,27(2):287-292.
[39] 贾贺威.龙须菜多糖抗肿瘤活性研究[D].硕士学位论文.天津:天津科技大学,2016. JIA H W.The research on antitumor activity of polysaccharides from Gracilaria Lemaneiformis[D].Master's Thesis.Tianjin:Tianjin University of Science and Technology,2016.(in Chinese)
[40] OKAI Y,HIGASHI-OKAI K,ISHIZAKA S,et al.Possible immunodulating activities in an extract of edible brown alga,Hijikia fusiforme (Hijiki)[J].Journal of the Science of Food and Agriculture,1998,76(1):56-62.  
[41] CHOI J H,KIM D I,PARK S H,et al.Effects of Sen tangle (Laminaria japonica) extract and fucoidan components on lipid metabolism of stressed mouse[J].Korean Journal of Fisheries and Aquatic Sciences,2000,33(2):124-128.
[42] BRITO T V,NETO J P R P,PRUDNCIO R S,et al.Sulfated-polysaccharide fraction extracted from red algae Gracilaria birdiae ameliorates trinitrobenzenesulfonic acid-induced colitis in rats[J].Journal of Pharmacy and Pharmacology,2014,66(8):1161-1170.  
[43] SANJEEWA K K A,FERNANDO I P S,KIM E A,et al.Anti-inflammatory activity of a sulfated polysaccharide isolated from an enzymatic digest of brown seaweed Sargassum horneri in RAW 264.7 cells[J].Nutrition Research and Practice,2017,11(1):3-10.  
[44] JOSE G M,KURUP G M.The efficacy of sulfated polysaccharides from Padina tetrastromatica in modulating the immune functions of RAW 264.7 cells[J].Biomedicine & Pharmacotherapy,2017,88:677-683.
[45] CHOI J I,RAGHAVENDRAN H R B,SUNG N Y,et al.Effect of fucoidan on aspirin-induced stomach ulceration in rats[J].Chemico-Biological Interactions,2010,183(1):249-254.  
Outlines

/