POULTRY NUTRITION AND FEED

Effects of Enzymatically Treated Artemisia annua Combined with Bacillus licheniformis on Growth Performance and Cecal Microbiota of Broilers

  • JIN Yaoyao ,
  • XU Bin ,
  • WANG Linyi ,
  • SUN Quanyou ,
  • XI Yanyan ,
  • YUAN Yanzhi ,
  • WANG Gaili ,
  • FU Chen ,
  • LI Shaoyu
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  • 1. Collage of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, China;
    2. Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Science, Zhengzhou 450002, China

Received date: 2020-12-09

  Online published: 2021-07-06

Supported by

 

Abstract

This experiment was conducted to investigate the effects of dietary enzymatically treated Artemisia annua combined with Bacillus licheniformis on the growth performance and cecal microbiota of broilers. A total of 480 Cobb broilers of one-day-old were randomly divided into 4 groups with 6 replicates per group and 20 broilers per replicate. Broilers in the control group were fed a basal diet, and those in the antibiotic group, trial group Ⅰ and trial group Ⅱ were fed the basal diets supplemented with 20 mg/kg antibiotics (a kind of virginiamycin premix), 1 g/kg enzymatically treated Artemisia annua and 1 g/kg enzymatically treated Artemisia annua+200 mg/kg Bacillus licheniformis, respectively. The experiment lasted for 42 days. The results showed as follows:1) compared with the control group, the average daily weight gain of broilers in the trial group Ⅱ was significantly increased (P<0.05), and which in the antibiotic group and trial group Ⅰ was increased by 5.18% and 7.49% (P>0.05), respectively; the final body weight of broilers in the trial group Ⅱ was significantly increased (P<0.05), and which in the antibiotic group and trial group Ⅰ was increased by 6.94% and 6.94% (P>0.05), respectively. There was no significant difference in average daily feed intake and the ratio of feed to gain among all groups (P>0.05). 2) The alpha diversity analysis in cecal microbiota of broilers found that the cecal microbiota diversity in the antibiotic group and both trial groups were significantly improved (P<0.05). The composition analysis in cecal microbiota of broilers in all 4 groups found that, at the phylum level, the dominant microbial phyla were mainly Firmicutes, Bacteroidetes and Tenericutes. Compared with the control group, the Firmicutes relative abundance in the antibiotic group and both trial groups was significantly increased (P<0.05), and the Bacteroidetes relative abundance in the trial group Ⅰ and trial groupⅡ was significantly decreased (P<0.05). At the genus level, compared with the control group, the Bacteroides relative abundance in the antibiotic group and both trial groups was significantly reduced (P<0.05), the Faecalibacterium relative abundance in the trial group Ⅰ and trial group Ⅱ was significantly increased (P<0.05), and the Ruminococcaceae_UCG-014 relative abundance in the trial group Ⅱ was significantly increased (P<0.05). It is concluded that enzymatically treated Artemisia annua has the potential of antibiotic substitutes in broiler diets. The addition of enzymatically treated Artemisia annua to the diet alone has a tendency to improve the performance of broilers. Enzymatically treated Artemisia annua combined with Bacillus licheniformis can improve the growth performance of broilers by improving the structure of cecal microbiota, increasing the richness and diversity of intestinal microbiota, and increasing the relative abundance ratio of Firmicutes to Bacteroides.

Cite this article

JIN Yaoyao , XU Bin , WANG Linyi , SUN Quanyou , XI Yanyan , YUAN Yanzhi , WANG Gaili , FU Chen , LI Shaoyu . Effects of Enzymatically Treated Artemisia annua Combined with Bacillus licheniformis on Growth Performance and Cecal Microbiota of Broilers[J]. Chinese Journal of Animal Nutrition, 2021 , 33(7) : 3810 -3820 . DOI: 10.3969/j.issn.1006-267x.2021.07.023

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