Effects of Acute Cold Stimulation on Duodenal Histology Structure and Nuclear Transcription Factor-&kappa;B/Intestinal Alkaline Phosphatase Signaling Pathway of Weaned Piglets

QIAO Xinyue; GAO Bingnan; REN Yulong; LI Jinku; CHEN Yan; CUI Yizhe; WANG Qiuju

doi:10.3969/j.issn.1006-267x.2021.07.046

2021 , Vol. 33 >Issue 7: 4049 - 4056

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.07.046

MOLECULAR AND CELLULAR NUTRITION

Effects of Acute Cold Stimulation on Duodenal Histology Structure and Nuclear Transcription Factor-κB/Intestinal Alkaline Phosphatase Signaling Pathway of Weaned Piglets

QIAO Xinyue ,
GAO Bingnan ,
REN Yulong ,
LI Jinku ,
CHEN Yan ,
CUI Yizhe ,
WANG Qiuju

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College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2021-01-07

Online published: 2021-07-06

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Abstract

The purpose of this study was to investigate the effects of acute cold stimulation on the duodenal histology structure and nuclear transcription factor-κB (NF-κB)/intestinal alkaline phosphatase (IAP) signaling pathway of weaned piglets. Sixteen healthy "Duroc×Landrace×Yorkshire" ternary hybrid weaned piglets at 30 days of age were selected and randomly divided into 4 groups with 4 replicates in each group and 1 piglet in each replicate. The control group was kept at (24±2)℃, while 3 acute cold stimulation groups were kept in an artificial climate chamber at (4±2)℃ at 2, 6 and 12 h, respectively. Piglets were slaughtered after the acute cold stimulation and duodenum was fixed, paraffin sectioned and hematoxylin-eosin (HE) stained. The morphology change of intestinal mucosa was observed in the sections, and the villi height and crypt depth were detected. Meanwhile, the expression of key proteins in the NF-κB/IAP signaling pathway in duodenum was detected by Western-blot method. The results showed that the duodenum of pigs in the control group had complete structure and close villi. In the acute cold stimulation groups, the villi were broken and a few epithelial cells denatured and shed. At 12 h under acute cold stimulation, the duodenal villi height of piglets was significantly lower than that of the control group and at 2 and 6 h under acute cold stimulation (P<0.05). Compared with the control group, the crypt depth and villi height/crypt depth in the 3 acute cold stimulation groups were decreased, but the differences were not significant (P>0.05). In the duodenum, the relative expression level of p65 was slightly increased compared with the control group at 2 h under acute cold stimulation, but the difference was not significant (P>0.05). At 6 h under acute cold stimulation, the relative expression level of p65 basically recovered to the level before cold stimulation (control group). At 12 h under acute cold stimulation, compared with the control group and at 6 h under acute cold stimulation, the relative expression level of p65 was significantly increased (P<0.05). Compared with the control group, the relative expression level of inhibitor of nuclear transcription factor-κB (IκB) at 2 and 6 h under acute cold stimulation showed an increased trend, with no significant difference (P>0.05). After 12 h under acute cold stimulation, the relative expression level of IκB increased extremely significantly compared with the control group (P<0.01), and compared with at 2 and 6 h under acute cold stimulation, it also increased significantly (P<0.01). Compared with the control group, the relative expression level of phosphorylated IκB (p-IκB) at 2 and 6 h under acute cold stimulation was slightly increased, with no significant difference (P>0.05). Compared with the control group, the relative expression level of p-IκB was extremely significantly increased at 12 h under acute cold stimulation (P<0.01). Compared with the control group, the relative expression level of IAP was extremely significantly increased at 2, 6 and 12 h under acute cold stimulation (P<0.01), and the relative expression level of IAP was extremely significantly decreased at 6 and 12 h under acute cold stimulation compared with 2 h under acute cold stimulation (P<0.01). The results indicated that acute cold stimulation can affect the intestinal mucosal structure of the duodenum, which may be related to the activation of NF-κB/IAP signaling pathway and the up-regulation of NF-κB/IAP signaling pathway related proteins by acute cold stimulation.

Key words： acute cold stimulation; weaned piglets; duodenum; NF-κ; B signaling pathway; IAP signaling pathway

Cite this article

QIAO Xinyue , GAO Bingnan , REN Yulong , LI Jinku , CHEN Yan , CUI Yizhe , WANG Qiuju . Effects of Acute Cold Stimulation on Duodenal Histology Structure and Nuclear Transcription Factor-κB/Intestinal Alkaline Phosphatase Signaling Pathway of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2021 , 33(7) : 4049 -4056 . DOI: 10.3969/j.issn.1006-267x.2021.07.046

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