Effects of Artemisia ordosica Polysaccharide on Immune Indexes and Expression of Related Genes and Proteins in Duodenum of Immune-Stressed Broilers Challenged by Lipopolysaccharide

XING Yuanyuan; ZHENG Yankai; GUO Shiwei; WANG Junli; JIN Xiao; XU Yuanqing; SHI Binlin

doi:10.3969/j.issn.1006-267x.2021.07.048

2021 , Vol. 33 >Issue 7: 4068 - 4078

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.07.048

MOLECULAR AND CELLULAR NUTRITION

Effects of Artemisia ordosica Polysaccharide on Immune Indexes and Expression of Related Genes and Proteins in Duodenum of Immune-Stressed Broilers Challenged by Lipopolysaccharide

XING Yuanyuan ,
ZHENG Yankai ,
GUO Shiwei ,
WANG Junli ,
JIN Xiao ,
XU Yuanqing ,
SHI Binlin

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College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2020-12-20

Online published: 2021-07-06

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Abstract

This experiment investigated the mitigative effect of Artemisia ordosica polysaccharide (AOP) on duodenal inflammation of immune-stressed broilers challenged by lipopolysaccharide (LPS) and its possible mechanism. A total of 192 one-day-old Arbor Acres (AA) broilers were randomly allotted to four treatments with 6 replicates and each replicate had 8 broilers. The experiment lasted for 42 d, and it included preliminary phase (days 1 to 14), stress periods Ⅰ (days 15 to 28, including 7 d of injection period and 7 d of convalescent period), and stress period Ⅱ (days 28 to 42, including 7 d of injection period and 7 d of convalescent period). Broilers in treatments 1 and 2 were fed a basal diet, and those in treatments 3 and 4 were fed an experiment diet (supplemented with 750 mg/kg AOP in the basal diet). During stress periods Ⅰ (days 15, 17, 19 and 21 of experiment) and Ⅱ (days 29, 31, 33 and 35 of experiment), broilers in treatments 1 and 3 were injected intra-abdominally with LPS solution, and those in treatments 2 and 4 were injected intra-abdominally with equal amount of sterile saline. At days 21 and 35, one broiler from each replicate was slaughtered to collect duodenum tissue, which was used to measure the immune indexes and the expression levels of related genes and proteins. The results showed that AOP supplementation significantly alleviated the decrease of growth performance induced by LPS (P<0.05). AOP supplementation significantly alleviated the overproduction of interleukin (IL)-1β (days 21 and 35), IL-6 (days 21 and 35) and IL-4 (day 21) in duodenum of broilers induced by LPS (P<0.05), and significantly alleviated the excessive decline of immunoglobulin M (IgM) content (day 21) in duodenum (P<0.05). Additionally, AOP supplementation significantly alleviated the over-expression of nuclear factor-κB p65 (NF-κB p65) (day 35) and IL-1β (day 21) genes in duodenum of broilers induced by LPS (P<0.05), as well as the nuclear factor-κB (NF-κB) signaling pathway related proteins (NF-κB p65, IL-1β and IL-6, days 21 and 35) (P<0.05), and significantly promoted the protein expression of NF-κB inhibitor α (IκBα) (P<0.05). Collectively, AOP can alleviate the excessive release of proinflammatory factors in duodenum of broilers induced by LPS by inhibiting the excessive activation of NF-κB signaling pathway.

Key words： Artemisia ordosica polysaccharide; broiler; immune stress

Cite this article

XING Yuanyuan , ZHENG Yankai , GUO Shiwei , WANG Junli , JIN Xiao , XU Yuanqing , SHI Binlin . Effects of Artemisia ordosica Polysaccharide on Immune Indexes and Expression of Related Genes and Proteins in Duodenum of Immune-Stressed Broilers Challenged by Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2021 , 33(7) : 4068 -4078 . DOI: 10.3969/j.issn.1006-267x.2021.07.048

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