Comparison of Cecectomy, Canulation or Slaughter Methods to Determine Apparent Digestibilities of Amino Acid in Diet for Roosters

CHEN Jiang; YU Yao; ZOU Yi; DU Zhongyuan; WANG Yuming; TAN Huize; LIU Songbai; ZENG Shuli; HE Xi; ZHAO Feng

doi:10.3969/j.issn.1006-267x.2021.07.051

2021 , Vol. 33 >Issue 7: 4098 - 4109

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.07.051

EXPERIMENTAL METHOD AND ANIMAL

Comparison of Cecectomy, Canulation or Slaughter Methods to Determine Apparent Digestibilities of Amino Acid in Diet for Roosters

CHEN Jiang ,
YU Yao ,
ZOU Yi ,
DU Zhongyuan ,
WANG Yuming ,
TAN Huize ,
LIU Songbai ,
ZENG Shuli ,
HE Xi ,
ZHAO Feng

Expand

1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Wen's Food Group Co., Ltd., Xinxing 527439, China

Received date: 2021-01-08

Online published: 2021-07-06

Supported by

Fold

Abstract

This experiment was conducted to compare the contents of titanium dioxide (TiO₂) and amino acid and apparent digestibilities of amino acid in diet of excreta collected by cecectomy, ileal digesta collected by cannulation and slaughter methods, to provide a proper reference to determine apparent digestibilies of amino acid. One hundred and eighty-six yellow-feathered roosters (n=39 for the first batch, n=147 for the second batch; average body weight ranged from 1.40 to 1.52 kg)were cecectomized and 47 yellow-feathered roosters (average body was 1.51 kg) were surgically fitted with a cannula in the distal ileum, and the recovery after operation was observed. A single factor completely random deign was adopted, 3 treatments were excreta in cecectomy, ileal digesta in cannulation method and ileal digesta in slaughter. There were 6 replicates in each treatment and 2 roosters in each replicate of samples collected by cecectomy and cannulation methods; there were 6 replicates in each treatment and 3 roosters in each replicate of samples collected by slaughter method. The apparent digestibilities of amino acid of different sources of broilers digested samples was determined. The results showed as follows:1) there was no significant difference in body weight gain on days 7, 14 and 21 after operation between cecectomized roosters and cannulated roosters (P>0.05). The death and cull rates at 3 months after the operation of the first and second batch of cecectomized roosters and cannulated roosters were 23.1%, 20.4% and 33.9%, respectively. 2) There was a significant difference in TiO₂ content in excreta in cecectomy, ileal digesta in cannulation method and ileal digesta in slaughter method (P<0.05). The contents of cysteine, threonine and proline in excreta in cecectomy were significantly lower than those in ileal digesta in cannulation method (P<0.05), and the contents of histidine, lysine, glycine and phenylalanine were significantly higher than those in ileal digesta in cannulation method and ileal digesta in slaughter method (P<0.05). The contents of cysteine, threonine and proline in ileal digesta in cannulation method were significantly higher than those in ileal digesta in slaughter method (P<0.05). 3) The apparent digestibilities of cysteine, threonine, valine, aspartate and proline in diet determined with cecectomy total collection method were significantly higher than those determined with cannulation method (P<0.05), the apparent digestibilities of histidine, lysine, glycine and Phenylalanine in diet determined with cecectomy total collection method were significantly lower than those determined with slaughter method (P<0.05), and the apparent digestibilities of cysteine, histidine, lysine, threonine, valine, aspartic acid, glycine, phenylalanine, proline and total amino acid in diet determined with cannulation method were significantly lower than those determined with slaughter method (P<0.05). 4) Overall, the apparent digestibilities of amino acid in diet and their coefficient of variation determined with cecectomy and slaughter methods were close, and the coefficient of variation of apparent digestibilities of amino acid in diet determined with cannulation method was greater. These results indicate that the apparent digestibilities of amino acid determined with excreta collected by cecectomy is comparable with that determined with ileal digesta collected by slaughtered method, whereas ileal digesta collected by cannulated method is not recommended for determining the apparent digestibilities of amino acids.

Key words： cecectomize; ileal cannula; ileal digesta; apparent amino acid digestibilities

Cite this article

CHEN Jiang , YU Yao , ZOU Yi , DU Zhongyuan , WANG Yuming , TAN Huize , LIU Songbai , ZENG Shuli , HE Xi , ZHAO Feng . Comparison of Cecectomy, Canulation or Slaughter Methods to Determine Apparent Digestibilities of Amino Acid in Diet for Roosters[J]. Chinese Journal of Animal Nutrition, 2021 , 33(7) : 4098 -4109 . DOI: 10.3969/j.issn.1006-267x.2021.07.051

References

[1] SHEIKHHASAN B S,MORAVEJ H,SHIVAZAD M,ret al.Prediction of the total and standardized ileal digestible amino acid contents from the chemical composition of soybean meals of different origin in broilers[J].Poultry Science,2020,99(10):4947-4957.

[2] SHEIKHHASAN B S,MORAVEJ H,GHAZIANI F,et al.Relationship between chemical composition and standardized ileal digestible amino acid contents of corn grain in broiler chickens[J].Poultry Science,2020,99(9):4496-4504.

[3] LU P Y,WANG J,WU S G,et al.Standardized ileal digestible amino acid and metabolizable energy content of wheat from different origins and the effect of exogenous xylanase on their determination in broilers[J].Poultry Science,2020,99(2):992-1000.

[4] WOYENGO T A,KIARIE E,NYACHOTI C M.Metabolizable energy and standardized ileal digestible amino acid contents of expeller-extracted canola meal fed to broiler chicks[J].Poultry Science,2010,89(6):1182-1189.

[5] REZVANI M R.Standardisation of precaecal and total tract amino acid digestibility measurement in laying hens[D].Ph.D.Thesis.Halle-Wittenberg,Germany:Martin Luther University,2007.
[6] REZVANI M,KLUTH H,RODEHUTSCORD M.Comparison of amino acid digestibility determined prececally or based on total excretion of cecectomized laying hens[J].Poultry Science,2008,87(11):2311-2319.

[7] JOHNS D C,LOW C K,JAMES K A C.Comparison of amino acid digestibility using the ileal digesta from growing chickens and cannulated adult cockerels[J].British Poultry Science,1986,27(4):679-685.

[8] WARREN B E,FARRELL D J.The nutritive value of full-fat and defatted Australian rice bran.Ⅴ.The apparent retention of minerals and apparent digestibility of amino acids in chickens and adult cockerels fitted with ileal cannulae[J].Animal Feed Science and Technology,1991,34:323-342.
[9] KLUTH H,MEHLHORN K,RODEHUTSCORD M.Studies on the intestine section to be sampled in broiler studies on precaecal amino acid digestibility[J].Archives of Animal Nutrition,2005,59(4):271-279.

[10] OLUKOSI O A,BOLARINWA O A,COWIESON A J,et al.Marker type but not concentration influenced apparent ileal amino acid digestibility in phytase-supplemented diets for broiler chickens and pigs[J].Journal of Animal Science,2012,90(12):4414-4420.

[11] KADIM I T,MOUGHAN P J.Development of an heal amino acid digestibility assay for the growing chicken-effects of time after feeding and site of sampling[J].British Poultry Science,1997,38(1):89-95.

[12] ZUBER T,SIEGERT W,SALEHI H,et al.Variability of amino acid digestibility of lupin and pea grains in caecectomised laying hens[J].British Poultry Science,2019,60(3):229-240.

[13] BOURDILLON A,CARRÉ B,CONAN L,et al.European reference method for the in vivo determination of metabolisable energy with adult cockerels:reproducibility,effect of food intake and comparison with individual laboratory methods[J].British Poultry Science,1990,31(3):557-565.

[14] VAN LEEUWEN P,BABINSZKY L,VERSTEGEN M W A,et al.A procedure for ileostomisation of adult roosters to determine apparent ileal digestibility of protein and amino acids of diets:comparison of six diets in roosters and growing pigs[J].Livestock Production Science,2000,67(1/2):101-111.
[15] PAYNE W L,KIFER R R,SNYDER D G,et al.Studies of protein digestion in the chicken.1.Investigation of apparent amino acid digestibility of fish meal protein using cecectomized,adult male chickens[J].Poultry Science,1971,50(1):143-150.

[16] 赵峰,张子仪,侯水生.鸭的空肠食糜连续采集方法及其空肠套管的设计[J].畜牧兽医学报,2006,37(7):672-675. ZHAO F,ZHANG Z Y,HOU S S.Method for continous collection of jejunum digesta and jejunal cannula design for duck[J].Acta Veterinaria et Zootechnica Sinica,2006,37(7):672-675.(in Chinese)
[17] POURESLAMI R,BATAL A B,JUNG B.Effect of ileal sub-section and the method of collection of digesta on the determination of apparent ileal digestibility of amino acids in broilers[J].Animal Feed Science and Technology,2012,177(1/2):130-133.
[18] OSHO S O,BABATUNDE O O,ADEOLA O.Additivity of apparent and standardized ileal digestibility of amino acids in wheat,canola meal,and sorghum distillers dried grains with solubles in mixed diets fed to broiler chickens[J].Poultry Science,2019,98(3):1333-1340.

[19] MYERS W D,LUDDEN P A,NAYIGIHUGU V,et al.Technical note:a procedure for the preparation and quantitative analysis of samples for titanium dioxide[J].Journal of Animal Science,2004,82(1):179-183.

[20] WANG T T,ADEOLA O.Digestibility index marker type,but not inclusion level affects apparent digestibility of energy and nitrogen and marker recovery in growing pigs regardless of added oat bran[J].Journal of Animal Science,2018,96(7):2817-2825.

[21] KONG C,ADEOLA O.Evaluation of amino acid and energy utilization in feedstuff for swine and poultry diets[J].Asian-Australasian Journal of Animal Science,2014,27(7):917-925.

[22] SON J H,RAGLAND D,ADEOLA O.Quantification of digesta flow into the caeca[J].British Poultry Science,2002,43(2):322-324.

[23] BLOK M C,DEKKER R A.Table ‘standardized ileal digestibility of amino acids in feedstuffs for poultry’[R].Wageningen:Wageningen Livestock Research,2017.
[24] PARSONS C M.Influence of caecectomy on digestibility of amino acids by roosters fed distillers' dried grains with solubles[J].The Journal of Agricultural Science,1985,104(2):469-472.

[25] WANG Z Y,SHI S R,ZHOU Q Y,et al.The influence of caecectomy on amino acid availability of three feedstuffs for ganders[J].British Poultry Science,2008,49(2):181-185.

[26] ZUBER T.Variability of amino acid digestibility of cereal grains in laying hens[D].Ph.D.Thesis.Stuttgart,Germany:University of Hohenheim,2017.
[27] ZUBER T,RODEHUTSCORD M.Variability in amino acid digestibility and metabolizable energy of corn studied in cecectomized laying hens[J].Poultry Science,2017,96(6):1696-1706.

[28] SIEGERT W,GANZER C,KLUTH H,et al.Influence of feed provisioning prior to digesta sampling on precaecal amino acid digestibility in broiler chickens[J].Archives of Animal Nutrition,2018,72(3):190-204.

[29] KIM E J,UTTERBACK P L,PARSONS C M.Comparison of amino acid digestibility coefficients for soybean meal,canola meal,fish meal,and meat and bone meal among 3 different bioassays[J].Poultry Science,2012,91(6):1350-1355.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References