EXPERIMENTAL METHOD AND ANIMAL

Evaluation of Application Effects of Bovine Compound Microecologic Agent

  • ZHAO Jie ,
  • XU Jingru ,
  • MA Li ,
  • CHEN Jiawen ,
  • XIE Xin ,
  • LI Luxin ,
  • YANG Keli ,
  • FENG Shibin ,
  • LI Yu ,
  • WU Jinjie ,
  • WANG Xichun
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  • 1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
    2. Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China

Received date: 2020-12-30

  Online published: 2021-07-06

Supported by

 

Abstract

The aim of this study was to investigate the effects of bovine compound microecological agent on immune function, antioxidant capacity and intestinal flora structure of Dabie mountain yellow cattle. From the intestine of Dabie mountain cattle, the bacterial strains with probiotic potential were isolated and identified, and the compound microecological agent was prepared. Twelve healthy weaned Dabie mountain yellow cattle with similar body size were randomly divided into 3 groups with 4 cattle in each group. The cattle control group (group C), bacillus group (group B) and compound microecological agent group (group E) were fed with a basal diet, the basal diet supplemented with Bacillus subtilis powder, and the basal diet supplemented with bovine compound microecological agent, respectively. The experiment was conducted for 30 days. On days 0, 15 and 30 of the experiment, blood samples were collected from the jugular vein of 4 calves in each group, and the serum was separated for the detection of cytokines, immune and antioxidant indices. On day 30 of the experiment, cattle faces was collected for high-throughput sequencing to detect the abundance and structural changes of bacterial flora. The results showed as follows:1) six strains of bacillus and five strains of lactic acid bacteria were isolated from Dabie mountain yellow cattle, then Bacillus subtilis and Lactococcus lactis were selected to make compound microecological agent according to the results of acid and bile salt tolerance tests. 2) On days 15 and 30, compared with the group C, the serum immunoglobulin G (IgG) content in group B and group E was extremely significantly increased (P<0.01), and the serum immunoglobulin M (IgM) content in group E was extremely significantly higher than that in group C and group B (P<0.01). 3) On day 30, the contents of serum interleukin-2 (IL-2), interleukin-6 (IL-6) and interferon-γ (IFN-γ) in group E were significantly higher than that in group C (P<0.05). 4) On day 30, compared with group C, the serum total antioxidant capacity (T-AOC) and the activities of glutathione peroxidase (GSH-Px) and catalase (CAT) in group B and group E were significantly increased (P<0.05), the serum superoxide dismutase (SOD) activity in group E was extremely significantly increased (P<0.01), the serum SOD activity in group B was significantly increased (P<0.05), and the malondialdehyde (MDA) content in group B and group E was significantly decreased (P<0.05). 5) Compared with group C, the proportion of Bacteroidetes in group E and group B was increased, while the proportion of Proteobacteria was decreased. In conclusion, the bovine microecological agent prepared in this experiment can enhance the immune function and antioxidant capacity of body, and improve the intestinal flora structure of Dabie mountain yellow cattle.

Cite this article

ZHAO Jie , XU Jingru , MA Li , CHEN Jiawen , XIE Xin , LI Luxin , YANG Keli , FENG Shibin , LI Yu , WU Jinjie , WANG Xichun . Evaluation of Application Effects of Bovine Compound Microecologic Agent[J]. Chinese Journal of Animal Nutrition, 2021 , 33(7) : 4110 -4122 . DOI: 10.3969/j.issn.1006-267x.2021.07.052

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