Effects of Microbe-Derived Antioxidants on Oxidative Damage and Inflammatory Response in Liver of Mice Induced by Dextran Sulfate Sodium Salt

LI Li; MA Sheng; ZHANG Jing; XU Weina; XU Jianxiong

doi:10.3969/j.issn.1006-267x.2021.07.053

2021 , Vol. 33 >Issue 7: 4123 - 4132

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.07.053

EXPERIMENTAL METHOD AND ANIMAL

Effects of Microbe-Derived Antioxidants on Oxidative Damage and Inflammatory Response in Liver of Mice Induced by Dextran Sulfate Sodium Salt

LI Li ,
MA Sheng ,
ZHANG Jing ,
XU Weina ,
XU Jianxiong

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Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

Received date: 2020-12-16

Online published: 2021-07-06

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Abstract

This experiment was conducted to explore the dextran sulfate sodium salt (DSS) induced liver damage of mice and the relieving effects and possible mechanism of microbe-derived antioxidants (MA). Forty-eight 6-week-old C57BL/6J male mice were randomly divided into 4 groups with 12 mice in each group after 1 week of pre feeding. Each mouse in the control group and DSS group was given normal saline 0.020 mL/g BW by gavage every day, each mouse in the low-dose MA group (LMA group) was given 0.002 mL/g BW MA by gavage every day, and each mouse in the high-dose MA group (HMA group) group was given 0.003 mL/g BW MA by gavage every day. Mice in the control group drank double distilled water every day; mice in other groups drank double distilled water from 1 to 7 days, and drank double distilled water contained 3% DSS from 8 to 14 days. The experiment lasted for 14 days. 1) compared with the control group, the DSS group showed inflammatory cell infiltration and steatosis in the central venous duct area of liver tissue; the contents of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) in liver were significantly increased (P<0.01), the total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) activities and glutathione (GSH) content and total antioxidant capacity (T-AOC) in liver were significantly decreased (P<0.05 or P<0.01), and the liver mitochondrial membrane potential was significantly decreased (P<0.05). Compared with the DSS group, the DSS, MA treatment improved the inflammatory cell infiltration and steatosis in the central venous duct area of liver tissue; the liver H₂O₂ content of LMA group was significantly decreased (P<0.05), and the liver T-AOC and GSH-Px activity were significantly increased (P<0.05); the contents of H₂O₂ and MDA in liver of HMA group were significantly decreased (P<0.01), and the T-SOD, GSH-Px activities and GSH content and T-AOC in liver were significantly increased (P<0.05); the liver mitochondrial membrane potential of LMA group and HMA group was significantly increased (P<0.05). 2) Compared with the control group, the mRNA relative expression levels of apoptosis-associated speck-like protein containing a CARD (ASC), cysteine aspartatespecific proteinase-1 (Caspase-1), interleukin-1β (IL-1β), extracellular regulated protein kinases (PERK), activating transcription factor 6 (ATF6), inositol requiring enzyme 1 (IRE1), glucose regulated protein 78 (GRP78) and telomerase reverse transcriptase (Tert) in liver of DSS group were significantly increased (P<0.05). Compared with the DSS group, the mRNA relative expression levels of ASC, Caspase-1, IL-1β and GRP78 in liver of HMA group were significantly decreased (P<0.05). In conclusion, DSS induces oxidative stress, injury and inflammation in liver of mice, MA have a significant alleviating effect on this, the alleviating effect of high-dose MA is stronger, and the Nod-like receptor family pyrin domain-containing protein 3 (NLRP3) inflammasome is involved in regulating inflammation.

Key words： microbe-derived antioxidants; DSS; liver; oxidative stress; NLRP3

Cite this article

LI Li , MA Sheng , ZHANG Jing , XU Weina , XU Jianxiong . Effects of Microbe-Derived Antioxidants on Oxidative Damage and Inflammatory Response in Liver of Mice Induced by Dextran Sulfate Sodium Salt[J]. Chinese Journal of Animal Nutrition, 2021 , 33(7) : 4123 -4132 . DOI: 10.3969/j.issn.1006-267x.2021.07.053

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