EXPERIMENTAL METHOD AND ANIMAL

Effects of Grape Seed Proanthocyanidins Extract on Fat Deposition and Lipid Metabolism in Rats

  • GU Aixin ,
  • ZHANG Tong ,
  • WANG Tianhu ,
  • WU Ze ,
  • SHAN Anshan ,
  • LI Jianping
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  • College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2020-12-13

  Online published: 2021-07-06

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Abstract

The aim of the present study was to evaluate the effects of grape seed proanthocyanidin extract (GSPE) on fat deposition and lipid metabolism in obese SD rats. A total of 52 female SD rats (170 to 220 g) were selected for the experiment, and they were randomly divided into control group (n=22) and high-fat group (n=30) after 1 week of adaptive feeding. A rat obesity model was established by feeding high-fat diets to the rats of high-fat group. After 8 weeks, the obesity model of the high-fat group was successfully established. After the obesity-resistant rats were eliminated, the control group was randomly divided into groups Ⅰ and Ⅱ, and the high-fat group was randomly divided into groups Ⅲ and Ⅳ, each group had 10 repetitions and 1 rat for each repeat. The GSPE was dissolved in normal saline, rats in groups Ⅰ and Ⅲ were gavaged with normal saline, rats in groups Ⅱ and Ⅳ were gavaged with GSPE solution, and each group was gavaged at a dose of 500 mg/kg BW GSPE. The trial period was 5 weeks. The results showed as follows:1) there was a significant interaction effect between the high-fat diet and the GSPE on liver weight (P<0.05), and there was a trend of interaction effect on the influence of white adipose tissue (WAT) weight (0.05<P<0.10). GSPE significantly reduced the body weight, liver weight and WAT weight of obese rats (P<0.05), and had no significant effect on the weight of spleen and kidney (P>0.05). 2) There was a significant interaction effect between the high-fat diet and the GSPE on high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) contents in serum (P<0.05). GSPE significantly increased the HDL-C content in the serum of obese rats and significantly decreased the LDL-C content (P<0.05). 3) There was a significant interaction effect between the high-fat diet and the GSPE on the relative expression level of peroxisome proliferator activates receptor γ (PPARγ) mRNA in WAT (P<0.05), and there was a trend of interaction effect on the relative expression level of fatty acid synthetase (FAS)mRNA (0.05<P<0.10). GSPE significantly downregulated the mRNA expression levels of fat synthesis genes (PPARγ and FAS) in the WAT of obese rats (P<0.05). 4) There was a significant interaction effect between the high-fat diet and the GSPE on the contents of Wnt3a and β-catenin in WAT (P<0.05). GSPE significantly downregulated the contents of Wnt3a and β-catenin in the WAT of obese rats (P<0.05). It can be speculated that GSPE supplementation promotes lipid metabolism and inhibit fat deposition in obese SD model rats. In addition, the lipid-lowering effect of GSPE may be related to the Wnt3a/β-catenin signaling pathway.

Cite this article

GU Aixin , ZHANG Tong , WANG Tianhu , WU Ze , SHAN Anshan , LI Jianping . Effects of Grape Seed Proanthocyanidins Extract on Fat Deposition and Lipid Metabolism in Rats[J]. Chinese Journal of Animal Nutrition, 2021 , 33(7) : 4133 -4141 . DOI: 10.3969/j.issn.1006-267x.2021.07.054

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