SWINE NUTRITION AND FEED

Effects of Dietary Lentinan on Ileal Barrier Function of Weaned Pigs Challenged by Rotavirus

  • MAO Xiangbing ,
  • HU Haiyan ,
  • CHEN Daiwen ,
  • YU Bing ,
  • HE Jun ,
  • ZHENG Ping ,
  • YU Jie ,
  • LUO Junqiu ,
  • LUO Yuheng ,
  • YAN Hui
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  • Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Key Laboratory of Animal Disease-Resistant Nutrition and Feed of Ministry of Agriculture and Rural Affairs, Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2021-01-29

  Online published: 2021-08-11

Supported by

 

Abstract

This experiment was conducted to determine whether dietary lentinan can alleviate the ileal barrier function of weaned pigs challenged by rotavirus (RV). Twenty-eight healthy crossbred (Duroc×Landrace×Yorkshire) piglets weaned at 21 days of age were used in a 2×2 factorial design. The trial factors included diets (control diet or supplementing with 84 mg/kg lentinan diet) and RV challenge (oral gavage with RV or sterilized medium). On day 15, the RV challenge was carried out. The trial duration was 19 days. The results showed that RV infection increased the diarrhea index of weaned pigs, significantly elevated the serum malondialdehyde (MDA) content and the contents of rotavirus antibody, interleukin-2 (IL-2) and MDA in ileal mucosa (P<0.05), significantly up-regulated the B-cell lymphoma/leukaemia 2-associated X protein (Bax) mRNA relative expression level in ileal mucosa (P<0.05), significantly decreased the serum total antioxidant capacity (T-AOC) and the contents of secreting immunoglobulin A (sIgA), interleukin-4 (IL-4) and interferon γ (IFN-γ) as well as T-AOC in ileal mucosa (P<0.05), significantly down-regulated the mRNA relative expression levels of B-cell lymphoma/leukaemia 2 (Bcl-2), zonula occludens 1 (ZO-1), occludin, mucin 1, mucin 2, porcine β-defensin 1 (pBD1), porcine β-defensin 2 (pBD2), porcine β-defensin 3 (pBD3) and protegrins 1-5 (PG1-5) in ileal mucosa (P<0.05), and significantly reduced the populations of Lactobacillus, Bifidobacterium and total bacteria in ileal digesta (P<0.05). Dietary lentinan reduced the diarrhea index of the RV-infected pigs, and significantly alleviated the effects of RV challenge on the contents of sIgA and cytokines (such as IL-2 and IFN-γ), the mRNA expressions of gut health related genes (including occludin, ZO-1, mucin 1, mucin 2, pBD2, pBD3 and PG1-5) and antioxidant capacity (P<0.05). These results suggest that dietary lentinan can improve ileum barrier function of weaned pigs infected with RV to some extent, which plays an important role in alleviating diarrhea induced by RV challenge.

Cite this article

MAO Xiangbing , HU Haiyan , CHEN Daiwen , YU Bing , HE Jun , ZHENG Ping , YU Jie , LUO Junqiu , LUO Yuheng , YAN Hui . Effects of Dietary Lentinan on Ileal Barrier Function of Weaned Pigs Challenged by Rotavirus[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4323 -4331 . DOI: 10.3969/j.issn.1006-267x.2021.08.013

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