SWINE NUTRITION AND FEED

Digestive Characteristics of Ileum and Colon in Bama Xiang Pigs

  • MO Jiayuan ,
  • LU Yujie ,
  • QI Wenjing ,
  • ZHU Siran ,
  • FENG Lingli ,
  • GAO Jiuyu ,
  • LIANG Liang ,
  • WANG Hui ,
  • LAN Ganqiu ,
  • LIANG Jing
Expand
  • College of Animal Science & Technology, Guangxi University, Nanning 530004, China

Received date: 2021-01-31

  Online published: 2021-08-11

Supported by

 

Abstract

This experiment was conducted to investigate the digestive characteristics of ileum and colon in Bama Xiang pigs. The ileum and colon contents of nine adult Bama Xiang sows were collected and detected by ultra-high performance liquid chromatography-tandem mass spectrometry. Compund Disover 3.1 software was used for metabolite detection, and the significant difference metabolites were screen by SIMCA-P and SPSS 19.0 software. MetaboAnalyst 5.0,HMDB and KEGG websites were used to analyze the metabolite properties and metabolic pathways. The results showed that a total of 26 119 and 16 265 peaks were obtained in positive and negative ion modes, and 6 403 and 1 912 metabolites were matched, respectively. The R2X model interpretation of PCA analysis was more than 0.6 and all the nine QC samples were concentrated in the center. The R2Y and Q2 of OPLS-DA analysis were more than 0.93 and the intercepts of Q2 verified by 200 iterations were all less than 0.05. A total of 42 metabolites with extremely significant difference were detected, of which 10 were related to bile acids and 6 were amino acids, and 14 different metabolites were involved in 19 metabolic pathways. The content of bile acids in ileum contents was 90 to 20 574 times higher than that in colon, and the content of amino acids in ileum contents was 5 to 21 times higher than that in colon. Instead, both dodecanedioic acid and 3-methyldioxyindole were about 1 000 times higher in colonic contents than in ileal contents. The results indicate that the high absorption efficiency of bile acids and amino acids are the digestive characteristics of ileum, and high microbial metabolites is the characteristic of colon digestion.

Cite this article

MO Jiayuan , LU Yujie , QI Wenjing , ZHU Siran , FENG Lingli , GAO Jiuyu , LIANG Liang , WANG Hui , LAN Ganqiu , LIANG Jing . Digestive Characteristics of Ileum and Colon in Bama Xiang Pigs[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4342 -4352 . DOI: 10.3969/j.issn.1006-267x.2021.08.015

References

[1] 王爱德,兰干球,郭亚芬.广西巴马小型猪的培育[J].实验动物科学,2010,27(1):60-63. WANG A D,LAN G Q,GUO Y F.Genetic breeding of Guangxi Bama mini-pig[J].Laboratory Animal Science,2010,27(1):60-63.(in Chinese)
[2] ZHAI X R,HAN W Z,WANG M,et al.Exogenous supplemental NAD+ protect myocardium against myocardial ischemic/reperfusion injury in swine model[J].American Journal of Translational Research,2019,11(9):6066-6074.
[3] NING X Y,YANG K,SHI W,et al.Comparison of hypertrophic scarring on a red Duroc pig and a Guangxi mini Bama pig[J].Scars,Burns & Healing,2020,6:2059513120930903.
[4] GUO M,LIU J G,GUO F F,et al.Panax quinquefolium saponins attenuate myocardial dysfunction induced by chronic ischemia[J].Cellular Physiology and Biochemistry,2018,49(4):1277-1288.  
[5] ZHU T Y,AI J,NIE C H,et al.Feasibility of computed tomography-guided irreversible electroporation for porcine kidney ablation[J].Journal of Cancer Research and Therapeutics,2020,16(5):1125-1128.  
[6] 朱春红,陶志云,刘宏祥,等.鸭不同肠段内菌群结构分析与拟杆菌分布研究[J].畜牧兽医学报,2020,51(12):3001-3012. ZHU C H,TAO Z Y,LIU H X,et al.Analysis of microbiota structure and bacteroides distribution in different intestinal segments in duck[J].Acta Veterinaria et Zootechnica Sinica,2020,51(12):3001-3012.(in Chinese)
[7] 董博,王志林,魏文康,等.哺乳仔猪断奶前后肠道微生物培养组学研究[J].动物营养学报,2021,33(1):175-189. DONG B,WANG Z L,WEI W K,et al.Study on intestinal microbial culturomics of piglets before and after weaning[J].Chinese Journal of Animal Nutrition,2021,33(1):175-189.(in Chinese)
[8] LIU W X,ZHANG R,SHU R,et al.Study of the relationship between microbiome and colorectal cancer susceptibility using 16SrRNA sequencing[J].BioMed Research International,2020,2020:7828392.
[9] LI Z,ZHU H,GUO Y X,et al.Gut microbiota regulate cognitive deficits and amyloid deposition in a model of Alzheimer's disease[J].Journal of Neurochemistry,2020,155(4):448-461.  
[10] ZUO T,ZHAN H,ZHANG F,et al.Alterations in fecal fungal microbiome of patients with COVID-19 during time of hospitalization until discharge[J].Gastroenterology,2020,159(4):1302-1310.e5.  
[11] PATTI G J,YANES O,SIUZDAK G.Innovation:metabolomics:the apogee of the omics trilogy[J].Nature Reviews Molecular Cell Biology,2012,13(4):263-269.  
[12] SINHA S R,HAILESELASSIE Y,NGUYEN L P,et al.Dysbiosis-induced secondary bile acid deficiency promotes intestinal inflammation[J].Cell Host & Microbe,2020,27(4):659-670.e5.  
[13] NICHOLSON J K,LINDON J C,HOLMES E.‘Metabonomics’:understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J].Xenobiotica,1999,29(11):1181-1189.  
[14] GABAZANA Z,SITOLE L.Raman-based metabonomics unravels metabolic changes related to a first-line tenofovir-based treatment in a small cohort of South African HIV-infected patients[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2021,248:119256.
[15] COELHO M O C,MONTEYNE A J,DIRKS M L,et al.Dailymycoprotein consumption for 1 week does not affect insulin sensitivity or glycaemic control but modulates the plasma lipidome in healthy adults:a randomised controlled trial[J].British Journal of Nutrition,2021,125(2):147-160.  
[16] AKHBARI P,JAGGARD M K,BOULANGÉ C L,et al.Differences between infected and noninfected synovial fluid[J].Bone & Joint Research,2021,10(1):85-95.  
[17] JI H N,SONG N N,REN J,et al.Metabonomics reveals bisphenol A affects fatty acid and glucose metabolism through activation of LXR in the liver of male mice[J].The Science of the Total Environment,2020,703:134681.
[18] HUO T G,FANG Y,ZHANG Y H,et al.Liver metabonomics study on the protective effect of glycyrrhetinic acid against realgar-induced liver injury[J].Chinese Journal of Natural Medicines,2020,18(2):138-147.  
[19] LIU Y,ZHANG X,GUAN T,et al.Effects of quercetin on cadmium-induced toxicity in rat urine using metabonomics techniques[J].Human & Experimental Toxicology,2020,39(4):524-536.  
[20] CHIANG J Y L,FERRELL J M.Bile acid receptors FXR and TGR5 signaling in fatty liver diseases and therapy[J].American Journal of Physiology-Gastrointestinal and Liver Physiology,2020,318(3):G554-G573.
[21] LI T G,CHIANG J Y L.Bile acid-based therapies for non-alcoholic steatohepatitis and alcoholic liver disease[J].Hepatobiliary Surgery and Nutrition,2020,9(2):152-169.  
[22] ZHENG X J,CHEN T L,JIANG R Q,et al.Hyocholic acid species improve glucose homeostasis through a distinct TGR5 and FXR signaling mechanism[J].Cell Metabolism,2021,33(4):791-803.e7.  
[23] CHANDRA R,LIDDLE R A.Cholecystokinin[J].Current Opinion in Endocrinology Diabetes and Obesity,2007,14(1):63-67.  
[24] HOFMANN A F.The enterohepatic circulation of bile acids in mammals:form and functions[J].Frontiers in Bioscience,2009,14:2584-2598.
[25] DAWSON P A,KARPEN S J.Intestinal transport and metabolism of bile acids[J].Journal of Lipid Research,2015,56(6):1085-1099.  
[26] THOMAS C,PELLICCIARI R,PRUZANSKI M,et al.Targeting bile-acid signalling for metabolic diseases[J].Nature Reviews Drug Discovery,2008,7(8):678-693.  
[27] YANG Y X,DAI Z L,ZHU W Y.Important impacts of intestinal bacteria on utilization of dietary amino acids in pigs[J].Amino Acids,2014,46(11):2489-2501.  
[28] 慕春龙,马梅蕾,何香玉,等. 体外法探究果寡糖对猪后肠微生物代谢苯丙氨酸和色氨酸的影响[J].畜牧兽医学报,2018,49(3):559-564. MU C L,MA M L,HE X Y,et al.Effect of FOS on metabolism of phenylalanine and tryptophan in pig hindgut bacteria fermentation broths in vitro[J].Acta Veterinaria et Zootechnica Sinica,2018,49(3):559-564.(in Chinese).
[29] 朱艳芝,马文锋,陈晓晨,等. 色氨酸分解代谢及其在猪饲粮中的应用进展[J].动物营养学报,2020,32(3):1019-1024. ZHU Y Z,MA W F,CHEN X C,etal.Tryptophan catabolism and its application in pig diets[J].Chinese Journal of Animal Nutrition,2020,32(3):1019-1024.(in Chinese).
[30] PHILIP M,SNOW R J,GATTA P A D,et al.Creatine metabolism in the uterus:potential implications for reproductive biology[J].Amino Acids,2020,52(9):1275-1283.  
[31] KAZAK L,COHEN P.Creatine metabolism:energy homeostasis,immunity and cancer biology[J].Nature Reviews Endocrinology,2020,16(8):421-436.  
[32] GRECO A V,MINGRONE G,CAPRISTO E,et al.The metabolic effect of dodecanedioic acid infusion in non-insulin-dependent diabetic patients[J].Nutrition,1998,14(4):351-357.  
[33] FUNK I,RIMMEL N,SCHORSCH C,et al.Production of dodecanedioic acid via biotransformation of low cost plant-oil derivatives using Candida tropicalis[J].Journal of Industrial Microbiology & Biotechnology,2017,44(10):1491-1502.  
[34] CAO W F,WANG Y J,LUO J Q,et al.Improving α,ω-dodecanedioic acid productivity from n-dodecane and hydrolysate of Candida cells by membrane integrated repeated batch fermentation[J].Bioresource Technology,2018,260:9-15.
[35] ALBRECHT C F,CHORN D J,WESSELS P L.Detection of 3-hydroxy-3-methyloxindole in human urine[J].Life Sciences,1989,45(12):1119-1126.  
Outlines

/