SWINE NUTRITION AND FEED

Effect of Dietary Bacillus coagulans on Growth Performance and Intestinal Health of Weaned Piglets Challenged with Enterotoxigenic Escherichia coli K88

  • LIU Chengdong ,
  • XIAO Mingxia ,
  • ZHAO Ping ,
  • LI Qihua ,
  • BAI Huayi ,
  • CHENG Zhibin
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  • College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China

Received date: 2021-01-20

  Online published: 2021-08-11

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Abstract

The purpose of this paper was to study the effects of dietary Bacillus coagulans on the growth performance, intestinal flora, morphology and cytokine expression of weaned piglets with enterotoxigenic Escherichia coli (ETEC) K88 challenge. Thirty-two healthy Duroc×Landrace×Yorkshire commercial boars weaned at 25 days of age were divided into 4 groups according to the body weight with 8 replicates per group and fed in single cages. Seven days before ETEC K88 infection, the piglets in two groups were fed a basal diet (BD group), and the piglets in the other two groups were fed the basal diet supplemented with 1.0×107 CFU/g Bacillus coagulans (PD group). From day 8, a 2×2 two-factor experiment was carried out, and the design of the four groups was as follows:1) NCH+BD group was given normal saline orally and fed the basal diet; 2) NCH+PD group was given normal saline orally and fed the Bacillus coagulans supplementation diet; 3) CH+BD group was given ETEC K88 orally and fed the basal diet; 4) CH+PD group was given ETEC K88 orally and fed the Bacillus coagulans supplementation diet. Pigs were given 5 mL ETEC K88 (viable count was 1.0×109 CFU/mL) or 5 mL 0.9% normal saline orally for 3 days. The Bacillus coagulans used in this experiment was a strain with good antibacterial effects on ETEC K88, which was determined by Oxford cup method in vitro, and the supplemental level was 1.0×107 CFU/g. On day 11 of the experiment, the samples of jejunum, ileum, cecum and their ingesta of piglets were taken to determine intestinal flora, morphology and cytokine expression levels. The results showed as follows:1) ETEC K88 infection significantly decreased the average daily gain and average daily feed intake, Lactobacillus number in jejunal and cecal ingesta, villus height in jejunum and ileum, and interleukin-10 (IL-10) mRNA expression level in jejunal mucosa of weaned piglets (P<0.05), while significantly increased the ratio of feed to gain, diarrhea index, Escherichia coli number in jejunal and cecal ingesta, crypt depth in jejunum and ileum, and interleukin-6 (IL-6) mRNA expression level in jejunal mucosa of weaned piglets (P<0.05). 2) Dietary Bacillus cloagulans significantly increased the average daily gain and average daily feed intake, Lactobacillus number of in jejunal and cecal ingesta, villus height in jejunum and ileum, and IL-10 mRNA expression level in jejunal mucosa of weaned piglets (P<0.05), while significantly decreased the ratio of feed to gain, diarrhea index, Escherichia coli number in jejunal and cecal ingesta, crypt depth in jejunum and ileum and IL-6 mRNA expression level in jejunal mucosa of weaned piglets (P<0.05). 3) There was no significant interaction effect between ETEC K88 infection and dietary Bacillus coagulans treatment on the other indices except diarrhea index (P>0.05). In conclusion, dietary Bacillus coagulans can improve the growth performance of weaned piglets by balancing intestinal flora, maintaining normal intestinal morphology and relieving intestinal inflammation regardless of the ETEC K88 challenge.

Cite this article

LIU Chengdong , XIAO Mingxia , ZHAO Ping , LI Qihua , BAI Huayi , CHENG Zhibin . Effect of Dietary Bacillus coagulans on Growth Performance and Intestinal Health of Weaned Piglets Challenged with Enterotoxigenic Escherichia coli K88[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4373 -4383 . DOI: 10.3969/j.issn.1006-267x.2021.08.018

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