Effects of Antimicrobial Peptide, Acidifier and Functional Oligosaccharide Combined Supplementation on Growth Performance, Intestinal Morphology and Cecal Microflora of Broilers

ZHU Suiliang; LI Yue; ZHENG Xu; ZHANG Jingjing; WANG Shiqiong; ZHANG Wei; TAN Jing; WANG Fengjiu; QIAO Yingying; LIU Changzhong; WANG Zhixiang

doi:10.3969/j.issn.1006-267x.2021.08.023

2021 , Vol. 33 >Issue 8: 4424 - 4437

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.08.023

POULTRY NUTRITION AND FEED

Effects of Antimicrobial Peptide, Acidifier and Functional Oligosaccharide Combined Supplementation on Growth Performance, Intestinal Morphology and Cecal Microflora of Broilers

ZHU Suiliang ,
LI Yue ,
ZHENG Xu ,
ZHANG Jingjing ,
WANG Shiqiong ,
ZHANG Wei ,
TAN Jing ,
WANG Fengjiu ,
QIAO Yingying ,
LIU Changzhong ,
WANG Zhixiang

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1. College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, China;
2. Henan Jiawang Biotechnology Co., Ltd., Zhengzhou 450002, China;
3. Henan Institute of Science and Technology, Xinxiang 453000, China

Received date: 2021-01-24

Online published: 2021-08-11

Supported by

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Abstract

This experiment was conducted to investigate the effects of antimicrobial peptide, acidifier and functional oligosaccharide combined supplementation instead of antibiotics on growth performance, serum immune indices, intestinal morphology and cecal microflora of broilers. A total of 720 one-day-old Arbor Acres broilers were randomly divided into 6 groups with 6 replicates in each group and 20 chickens (half male and half female) in each replicate. Broilers in the control group (NC group) were fed a basal diet, and those in the antibiotics group (AN group) were fed the basal diet supplemented with 500 mg/kg oxytetracycline calcium (based on 50 mg/kg oxytetracycline active ingredient), and the others in the experimental groups were fed the basal diets supplemented with 300 mg/kg antimicrobial peptide+200 mg/kg functional oligosaccharide (AP+FOS group), 300 mg/kg antimicrobial peptide+350 mg/kg acidifier (AP+AC group), 350 mg/kg acidifier+200 mg/kg functional oligosaccharide (AC+FOS group) and 300 mg/kg antimicrobial peptide+350 mg/kg acidifier+200 mg/kg functional oligosaccharide (AP+AC+FOS group), respectively. The experiment lasted for 42 days. The results showed as follows:1) compared with the NC group, the body weight of broilers at 42 days of age in the AN group, AP+FOS group, AP+AC group, AC+FOS group and AP+AC+FOS group was significantly increased (P<0.05), the average daily gain (ADG) was significantly increased at 22 to 42 days and 1 to 42 days of age (P<0.05), while the feed to gain ratio (F/G) was significantly decreased at 22 to 42 days and 1 to 42 days of age (P<0.05); the F/G of broilers at 1 to 21 days of age in the AP+FOS group was significantly decreased (P<0.05). Compared with the AN group, there were no significant differences in ADG, average daily feed intake and F/G among AP+FOS group, AP+AC group, AC+FOS group and AP+AC+FOS group in each period (P>0.05). 2) Compared with the NC group and AN group, the serum immunoglobulin A content of broilers in the AP+FOS group, AP+AC group and AP+AC+FOS group was significantly increased (P<0.05), and the serum immunoglobulin G content of broilers in the AP+FOS group, AP+AC group and AC+FOS group was significantly increased (P<0.05). Compared with the NC group, the serum immunoglobulin M content of broilers in the AN group, AP+FOS group, AP+AC group, AC+FOS group and AP+AC+FOS group was significantly increased (P<0.05). 3) Compared with the NC group, the crypt depth in duodenum and ileum of broilers in the AN group, AP+FOS group, AP+AC group, AC+FOS group and AP+AC+FOS group was significantly decreased (P<0.05), the villus height to crypt depth ratio (V/C) in duodenum of broilers in the AP+FOS group, AP+AC group, AC+FOS group and AP+AC+FOS group was significantly increased (P<0.05), and the V/C in ileum of broilers in the AP+FOS group, AP+AC group and AP+AC+FOS group was significantly increased (P<0.05). 4) Compared with the NC group and AN group, the ACE index, Chao1 index and Shannon index in cecal microflora of broilers in the AP+FOS group, AP+AC group, AC+FOS group and AP +AC+FOS group were increased to some extent. Compared with the NC group, the relative abundance of Ruminococcaceae_UCG-014 and Synergistes in cecum of broilers in the AP+FOS group, AP+AC group, AC+FOS group and AP+AC+FOS group was increased. In conclusion, combined supplementation of antimicrobial peptide, acidifier and functional oligosaccharide can improve the growth performance and immune performance of broilers, ameliorate the morphology of small intestine, regulate the diversity and abundance of cecal microorganisms, and promote the intestinal health of broilers. The combination of antimicrobial peptide with acidifier or functional oligosaccharide has a better effect on immune performance and has the potential to replace antibiotics.

Key words： broilers; antimicrobial peptide; acidifier; functional oligosaccharide; intestinal morphology; cecal microflora

Cite this article

ZHU Suiliang , LI Yue , ZHENG Xu , ZHANG Jingjing , WANG Shiqiong , ZHANG Wei , TAN Jing , WANG Fengjiu , QIAO Yingying , LIU Changzhong , WANG Zhixiang . Effects of Antimicrobial Peptide, Acidifier and Functional Oligosaccharide Combined Supplementation on Growth Performance, Intestinal Morphology and Cecal Microflora of Broilers[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4424 -4437 . DOI: 10.3969/j.issn.1006-267x.2021.08.023

References

[1] 张利珍.抗生素残留危害及预防措施[J].畜牧兽医科学(电子版),2019(5):54-55. ZHANG L Z.Antibiotic residue hazard and preventive measures[J].Graziery Veterinary Sciences (Electronic Version),2019(5):54-55.(in Chinese)
[2] 孙得发,李超,郭志有,等.抗生素替代品在817小型商品肉鸡日粮中的应用研究[J].家禽科学,2020(12):18-22. SUN D F,LI C,GUO Z Y,et al.Study on the application of antibiotic substitutes in the diet of 817 small commercial broilers[J].Poultry Science,2020(12):18-22.(in Chinese)
[3] 黄小建,李跃龙,戚南山,等.抗菌肽的生物学活性及其在畜禽生产中的作用[J].动物医学进展,2020,41(12):115-119. HUANG X J,LI Y L,QI N S,et al.Biological activity of antimicrobial peptides and their roles in livestock and poultry production[J].Progress in Veterinary Medicine,2020,41(12):115-119.(in Chinese)
[4] EFTEKHARI A,REZAEIPOUR V,ABDULLAHPOUR R.Effects of acidified drinking water on performance,carcass,immune response,jejunum morphology,and microbiota activity of broiler chickens fed diets containing graded levels of threonine[J].Livestock Science,2015,180:158-163.
[5] RAGAA N M,KORANY R M S.Studying the effect of formic acid and potassium diformate on performance,immunity and gut health of broiler chickens[J].Animal Nutrition,2016,2(4):296-302.

[6] CHAVEERACH P,KEUZENKAMP D A,LIPMAN L J A,et al.Effect of organic acids in drinking water for young broilers on Campylobacter infection,volatile fatty acid production,gut microflora and histological cell changes[J].Poultry Science,2004,83(3):330-334.

[7] 乔宇,田丹丹,徐小轻,等.功能性寡糖在动物健康养殖中的应用研究进展及现状[J].生物产业技术,2018(6):35-39. QIAO Y,TIAN D D,XU X Q,et al.Research progress and application on functional oligosaccharides in animal healthy breeding[J].Biotechnology & Business,2018(6):35-39.(in Chinese)
[8] 杨季,王思博,鲁茗源,等.橙皮苷对肉仔鸡生长性能、肌肉品质及肠道形态结构的影响[J].动物营养学报,2021,33(1):297-307. YANG J,WANG S B,LU M Y,et al.Effects of hesperidin on growth performance,muscle quality and intestinal morphology of broilers[J].Chinese Journal of Animal Nutrition,2021,33(1):297-307.(in Chinese)
[9] 苗旭,刘彦,贺军,等.抗菌肽对肉鸡生长性能的影响[J].国外畜牧学(猪与禽),2020,40(10):32-37. MIAO X,LIU Y,HE J,et al.Effect of antimicrobial peptides on growth performance of Broilers[J].Animal Science Abroad (Pigs and Poultry),2020,40(10):32-37.(in Chinese)
[10] 刘松柏,谭会泽,温志芬,等.无抗日粮添加酸化剂对黄羽肉鸡生长性能与肠道健康状况的影响[J].粮食与饲料工业,2020(5):36-38. LIU S B,TAN H Z,WEN Z F,et al.Effects of adding acidifier on growth performance and intestinal health of yellow chicken with antibiotic-free diet[J].Cereal & Feed Industry,2020(5):36-38.(in Chinese)
[11] 史宇涛,马志鹏,张俊梅,等.壳寡糖对白羽肉鸡生长性能及血清生化指标和肠道形态的影响[J].武汉轻工大学学报,2020,39(3):8-12,22. SHI Y T,MA Z P,ZHANG J M,et al.Effect of chitooligosaccharides on growth performance,serum biochemical indices and intestinal morphology of broilers[J].Journal of Wuhan Polytechnic University,2020,39(3):8-12,22.(in Chinese)
[12] 黄剑韬,吴雪梅,江绮汶,等.酸化剂、微生态制剂和寡糖对‘麒麟鸡’生长性能和消化道酶活力的影响[J].中国农学通报,2015,31(23):19-24. HUANG J T,WU X M,Jiang Q W,et al.Effects of acidifier,probiotics and oligosaccharide on growth performance and digestive enzyme activity of ‘Frizzled’ chicken[J].Chinese Agricultural Science Bulletin,2015,31(23):19-24.(in Chinese)
[13] 余绍海.甘露寡糖、抗菌肽在肉用番鸭生产中的应用研究[D].硕士学位论文.扬州:扬州大学,2006. YU S H.Application of mannose-oligosaccharides and antimicrobial peptides in meat muscovy production[D].Master's Thesis.Yangzhou:Yangzhou University,2006.(in Chinese)
[14] 李陇梅.抗菌肽对断奶仔猪生长性能、免疫机能及粪便微生物菌群的影响[J].中国饲料,2020(15):45-48. LI L M.Effects of antimicrobial peptides on growth performance,immune function and fecal microflora of weaned piglets[J].China Feed,2020(15):45-48.(in Chinese)
[15] 熊娟,王标,杨红军,等.植物精油以及与酸化剂组合对肉鸡生长性能和免疫指标的影响[J].饲料工业,2019,40(24):8-13. XIONG J,WANG B,YANG H J,et al.Effects of plant essential oil and acidifier on growth performance and immune index of broilers[J].Feed Industry,2019,40(24):8-13.(in Chinese)
[16] 夏青,梁馨元,张琳依,等.功能性寡糖调控肠道健康的研究进展[J/OL].食品工业科技,2021:1-11.(2021-01-19)[2021-02-08].https://doi.org/10.13386/j.issn1002-0306.2020100019. XIA Q,LIANG X Y,ZHANG L Y,et al.Research progress of functional oligosaccharides on regulating intestinal health[J/OL].Science and Technology of Food Industry:2021:1-11.(2021-01-19)[2021-02-08].https://doi.org/10.13386/j.issn1002-0306.2020100019. (in Chinese)
[17] 毕冉,代曼曼,曹伟胜,等.禽类免疫球蛋白[J].养禽与禽病防治,2019(2):5-9. BI R,DAI M M,CAO W S,et al.Immunoglobulins in poultry[J].Poultry Husbandry and Disease Control,2019(2):5-9.(in Chinese)
[18] FATHI M M,EBEID T A,AL-HOMIDAN I,et al.Influence of probiotic supplementation on immune response in broilers raised under hot climate[J].British Poultry Science,2017,58(5):512-516.

[19] 吴东,杨家军,吴义景,等.添加不同比例的抗菌肽替代饲用抗生素对肉鸡生长性能、血清免疫指标及肠道菌群的影响[J].粮食与饲料工业,2020(5):39-42. WU D,YANG J J,WU Y J,et al.Effect of feed antibiotics replaced by antimicrobial peptides on growth performance,serum immune index and intestinal flora of broilers[J].Cereal & Feed Industry,2020(5):39-42.(in Chinese)
[20] 田丽娜,王秀荣,李广兴.抗菌肽Sublancin对蛋鸡免疫功能的影响研究[J].中国预防兽医学报,2020,42(3):268-273. TAN L N,WANG X R,LI G X.Effect of antibacterial peptide Sublancin on the immune function of laying hens[J].Chinese Journal of Preventive Veterinary Medicine,2020,42(3):268-273.(in Chinese)
[21] 彭翔.抗菌肽和姜黄素对肉仔鸡生长性能、抗氧化功能、肠道微生物及免疫功能的影响[D].硕士学位论文.郑州:河南农业大学,2014. PENG X.Effects of cecropin antimicrobial peptide and curcumin on growth performance,antioxidant performance,intestinal microflora and immune function of briolers[D].Master's Thesis.Zhengzhou:Henan Agricultural University,2014.(in Chinese)
[22] LILBURN M S,LOEFFLER S.Early intestinal growth and development in poultry[J].Poultry Science,2015,94(7):1569-1576.

[23] CSERNUS B,CZEGLÉDI L.Physiological,antimicrobial,intestine morphological,and immunological effects of fructooligosaccharides in pigs[J].Archives Animal Breeding,2020,63(2):325-335.

[24] CHAMORRO S,ROMERO C,BRENES A,et al.Impact of a sustained consumption of grape extract on digestion,gut microbial metabolism and intestinal barrier in broiler chickens[J].Food & Function,2019,10(3):1444-1454.

[25] 王建.饲粮添加抗菌肽Api-PR19对肉鸡生长性能及肠道健康的影响[D].硕士学位论文.杨凌:西北农林科技大学,2019. WANG J.Effects of Api-PR19 on growth performance and intestine health of broiler chickens[D].Master's Thesis.Yangling:Northwest A&F University,2019.(in Chinese)
[26] 白洁.饲粮添加壳寡糖对肉鸡生产性能、免疫性能、抗氧化性能及肠道发育的影响[D].硕士学位论文.兰州:甘肃农业大学,2018. BAI J.Effects of chitosan oligosaccharide supplementation on production performance,immune performance,serum antioxidant activity and intestinal development in broilers[D].Master's Thesis.Lanzhou:Gansu Agricultural University,2018.(in Chinese)
[27] GIANNENAS I A,PAPANEOPHYTOU C P,TSALIE E,et al.The effects of benzoic acid and essential oil compounds in combination with protease on the performance of chickens[J].Journal of Animal and Feed Sciences,2014,23(1):73-81.

[28] XIAO H,SHAO F Y,WU M M,et al.The application of antimicrobial peptides as growth and health promoters for swine[J].Journal of Animal Science and Biotechnology,2016,6(1):19.
[29] 黄灵杰,张克英,白世平,等.苯甲酸对1-21日龄肉鸡生长性能和肠道健康的影响[J].动物营养学报,2019,31(6):2816-2822. HUANG L J,ZHANG K Y,BAI S P,et al.Effects of benzoic acid on growth performance and intestinal health of broilers at 1 to 21 days of age[J].Chinese Journal of Animal Nutrition,2019,31(6):2816-2822.(in Chinese)
[30] 黄永洁.低聚木糖对断奶仔猪肠道pH和肠黏膜形态结构的影响[J].现代畜牧兽医,2014(5):23-27. HUANG Y J.Effects of xylo-oligosaccharides on intestinal pH and intestinal mucosal morphological structure of weaned piglets[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2014(5):23-27.(in Chinese)
[31] LEE K W,EVERTS H,KAPPERT H J,et al.Effects of dietary essential oil components on growth performance,digestive enzymes and lipid metabolism in female broiler chickens[J].British Poultry Science,2003,44(3):450-457.

[32] 张雪,张珊,钟光,等.枯草芽孢杆菌对肉鸡生长性能、肠道组织形态和盲肠微生物组成的影响[J].动物营养学报,2020,32(11):5195-5208. ZHANG X,ZHANG S,ZHONG G,et al.Effects of Bacillus subtilis on growth performance,intestinal morphology and cecal microbial composition of broilers[J].Chinese Journal of Animal Nutrition,2020,32(11):5195-5208.(in Chinese)
[33] AHIR V B,KORINGA P G,BHATT V D,et al.Metagenomic analysis of poultry gut microbes[J].Indian Journal of Poultry Science,2010,45(2):111-114.
[34] OAKLEY B B,LILLEHOJ H S,KOGUT M H,et al.The chicken gastrointestinal microbiome[J].FEMS Microbiology Letters,2015,360(2):100-112.
[35] PANDIT R J,HINSU A T,PATEL N V,et al.Microbial diversity and community composition of caecal microbiota in commercial and indigenous Indian chickens determined using 16S rDNA amplicon sequencing[J].Microbiome,2018,6:115.
[36] HUWS S A,KIM E J,LEE M R F,et al.As yet uncultured bacteria phylogenetically classified as prevotella,lachnospiraceae incertae sedis and unclassified bacteroidales,clostridiales and ruminococcaceae may play a predominant role in ruminal biohydrogenation[J].Environmental Microbiology,2011,13(6):1500-1512.

[37] 金娜,邹闻书,高倩,等.枸芪多糖对育肥猪肠道菌群多样性及组成的影响[J].动物营养学报,2019,31(9):4033-4043. JIN N,ZOU W S,GAO Q,et al.Effects of Gou-Qi polysaccharides on intestinal microbial diversity and composition of growing-finishing pigs[J].Chinese Journal of Animal Nutrition,2019,31(9):4033-4043.(in Chinese)
[38] BALAMURUGAN R,RAJENDIRAN E,GEORGE S,et al.Real-time polymerase chain reaction quantification of specific butyrate-producing bacteria,Desulfovibrio and Enterococcus faecalis in the feces of patients with colorectal cancer[J].Journal of Gastroenterology and Hepatology,2008,23(8 Pt 1):1298-1303.
[39] LI S Y,WANG Z L,YANG Y,et al.Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress[J].AMB Express,2017,7(1):82.
[40] 魏海滨.甘露寡糖、抗菌肽和糖萜素在肉鸡饲料中添加效果的研究[D].硕士学位论文.福州:福建农林大学,2006. WEI H B.Effect of the dietary mannanoligosaccharides,antimicrobial peptides and saccharicterpenin on the broilers[D].Master's Thesis.Fuzhou:Fujian Agriculture and Forestry University,2006.(in Chinese)
[41] 马嘉瑜,朴香淑.酸化剂改善畜禽生长和肠道健康的研究进展[J/OL].中国畜牧杂志,2021:1-17.(2021-02-04)[2021-02-08].https://doi.org/10.19556/j.0258-7033.20201105-02. MA J Y,PIAO X S.Recent advances in acidifiers on growth performance and intestinal health of livestock and poultry[J/OL].Chinese Journal of Animal Science:2021:1-17.(2021-02-04)[2021-02-08].https://doi.org/10.19556/j.0258-7033.20201105-02. (in Chinese)

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