Study on Microbial Diversity of Yak Milk at Different Altitudes in Qinghai Area

MA Jing; WANG Xun; SUN Lu; LIU Shujie

doi:10.3969/j.issn.1006-267x.2021.08.029

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 8: 4491 - 4501

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.08.029

RUMINANT AND HERBIVORE NUTRITION AND FEED

Study on Microbial Diversity of Yak Milk at Different Altitudes in Qinghai Area

MA Jing ,
WANG Xun ,
SUN Lu ,
LIU Shujie

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1. Qinghai Academy of Science and Veterinary Medicine of Qinghai University, Xining 810016, China;
2. Qinghai Plateau Yak Research Center, Xining 810016, China;
3. Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Xining 810016, China

Received date: 2021-03-11

Online published: 2021-08-11

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Abstract

As a natural concentrated milk, yak milk provides nutrients needed for the growth of various microorganisms. To understand the microbial composition and structure of yak raw milk at different altitudes in alpine grasslands in Qinghai area of Qinghai-Tibet plateau, the 16S rDNA method was used to analyze the microbial diversity of yak milk which sampled at altitudes of 4 781 (yak1), 3 984 (yak2) and 3 372 (yak3) in this study, and 5 samples were collected in each sampling point. The results showed that a total of 12 329 operational taxonomic units (OTUs) were obtained at the level of 97% identity, and there were 2 988 OTUs in yak milk from three altitudes. There was no significant difference in microbial Alpha diversity indexes of yak milk among three altitudes (P>0.05). At phylum level, Proteobacteria and Firmicutes were the dominant bacteria phyla, and the two phyla in yak milk of the yak1 account for the bacterial sequence respectively 44.49% and 20.93% of the total, 39.30% and 32.66% in yak milk of the yak2, 41.32% and 23.90% in yak milk of the yak3. At genus level, the dominant genus in yak milk of the yak1 and yak3 was Acinetobacter, and the yak3 was Lactobacillus as the dominant genus. At species level, the dominant species in yak milk of the yak1 was Acinetobacter lwoffii, the dominant species of yak2 was Moraxella osloensis, and the dominant species of yak3 was Kosakonia oryzae. It can be seen that the microbial composition and abundance of yak milk at different altitudes in Qinghai area have differences.

Key words： yak milk; altitude; 16S rDNA; microbial diversity

Cite this article

MA Jing , WANG Xun , SUN Lu , LIU Shujie . Study on Microbial Diversity of Yak Milk at Different Altitudes in Qinghai Area[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4491 -4501 . DOI: 10.3969/j.issn.1006-267x.2021.08.029

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