Effects of Citric Acid on Growth Performance, Nutrient Utilization, Serum Biochemical Indices and Intestinal Tissue Morphology of Grass Carp (Ctenopharyngodon idella)

CHENG Zhuo; ZHANG Guoqi; ZHOU Lu; XU Zhen; YANG Hang; LI Xiaoqin; LENG Xiangjun

doi:10.3969/j.issn.1006-267x.2021.08.038

2021 , Vol. 33 >Issue 8: 4580 - 4591

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.08.038

AQUATIC ANIMAL NUTRITION AND FEED

Effects of Citric Acid on Growth Performance, Nutrient Utilization, Serum Biochemical Indices and Intestinal Tissue Morphology of Grass Carp (Ctenopharyngodon idella)

CHENG Zhuo ,
ZHANG Guoqi ,
ZHOU Lu ,
XU Zhen ,
YANG Hang ,
LI Xiaoqin ,
LENG Xiangjun

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1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
2. Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;
3. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China;
4. Shanghai Songjiang Aquatic Products Breeding Farm, Shanghai 201203, China

Received date: 2021-01-31

Online published: 2021-08-11

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Abstract

The study investigated the effects of citric acid on growth performance, nutrient utilization, serum biochemical indices and intestinal tissue morphology of grass carp (Ctenopharyngodon idella). Citric acid was supplemented into a basal diet at an inclusion of 0 (control group), 0.15%, 0.30% and 0.45%, and then the four isonitrogenous and isolipidemic diets were fed to grass carp[initial body weight (17.0±0.2) g] for 60 days. The results showed that 0.30% citric acid group had the highest weight gain rate (432.8%) and the lowest feed conversion ratio (1.30), which was 11.37% higher and 10.34% lower than the control group (P<0.05), respectively. The supplementation of 0.15% to 0.45% citric acid significantly increased the phosphorus apparent digestibility, phosphorus retention rate and lipid retention rate (P<0.05), and the supplementation of 0.30% citric acid group significantly increased the protein retention rate, dry matter apparent digestibility and protein apparent digestibility compared with the control group (P<0.05). Compared with the control group, the serum superoxide dismutase (SOD) activity in 0.15% and 0.30% citric acid groups was significantly increased, while the serum malondialdehyde (MDA) content was significantly decreased (P<0.05). The 0.30% and 0.45% citric acid groups showed higher intestinal protease and amylase activities compared with the control group (P<0.05). The intestinal villus height and muscle layer thickness in 0.15% to 0.45% citric acid groups were significantly higher than those in the control group (P<0.05), but there was no significant difference in villus width among all groups (P>0.05). In conclusion, dietary citric acid can promote the growth performance and feed utilization, enhance the serum antioxidant capacity, and improve the intestinal tissue morphology of grass carp, and the optimal supplemental level of citric acid in the diet of grass carp is 0.30%.

Key words： grass carp; citric acid; growth; digestibility; retention rate; serum biochemical indices; intestinal histology

Cite this article

CHENG Zhuo , ZHANG Guoqi , ZHOU Lu , XU Zhen , YANG Hang , LI Xiaoqin , LENG Xiangjun . Effects of Citric Acid on Growth Performance, Nutrient Utilization, Serum Biochemical Indices and Intestinal Tissue Morphology of Grass Carp (Ctenopharyngodon idella)[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4580 -4591 . DOI: 10.3969/j.issn.1006-267x.2021.08.038

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