Analysis of Muscle Nutritional Components of Different Sizes of Procambarus clarkii Cultured in Rice Field in Hunan Province

LUO Yating; LIU Yi; LIU Jinmeng; XIAO Tiaoyi; LIU Qiaolin

doi:10.3969/j.issn.1006-267x.2021.08.039

2021 , Vol. 33 >Issue 8: 4592 - 4603

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.08.039

AQUATIC ANIMAL NUTRITION AND FEED

Analysis of Muscle Nutritional Components of Different Sizes of Procambarus clarkii Cultured in Rice Field in Hunan Province

LUO Yating ,
LIU Yi ,
LIU Jinmeng ,
XIAO Tiaoyi ,
LIU Qiaolin

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1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Characteristic Aquatic Resources Utilization Engineering Technology Center, Hunan Agricultural University, Changsha 410128, China

Received date: 2021-01-26

Online published: 2021-08-11

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Abstract

In order to scientifically evaluate the nutritional characteristics of different sizes of Procambarus clarkii under the rice-shrimp co-culture mode, four sizes of 0 to 10 g (group A₁), 10 to 20 g (group A₂), 20 to 30 g (group A₃) and 30 to 50 g (group A₄) Procambarus clarkii cultured in Hunan rice field in August were taken as the research objects. The meat rate, hepatosomatic index and muscle nutritional component contents, texture characteristics, muscle fiber characteristics and meat-related gene expression levels of different sizes of Procambarus clarkii were measured. The results showed as follows:hepatosomatic index of Procambarus clarkii was significantly different between groups A₁ and A₄ (P<0.05), but there were no significant differences among other groups (P>0.05). The meat rate in group A₁ was significantly higher than that in the other three groups (P<0.05). The contents of muscle moisture, crude protein and ash were gradually decreased with the increase of sizes, and all in group A₁ were significantly higher than those in group A₄ (P<0.05). The muscle ether extract content was increased gradually with the increase of sizes, which was significantly lower in group A₁ than in groups A₃ and A₄ (P<0.05). A total of 17 amino acids were detected in muscle, including 7 essential amino acids and 4 delicious amino acids. The amino acid score (AAS) and chemical score (CS) of lysine in four groups were the highest. A total of 20 fatty acids were detected in muscle, including 7 saturated fatty acids, 3 monounsaturated fats and 10 polyunsaturated fatty acids. The content of total fatty acids in group A₁ was the highest and that in group A₃ was the lowest. 2) Muscle hardness, gumminess, springiness, chewiness and cohesiveness in group A₄ were the highest, and the chewiness was significantly different from other three groups (P<0.05). The smallest muscle fiber diameter and the largest muscle fiber density were found in group A₁, and the muscle fiber density in group A₁ was significantly higher than that in the other three groups (P<0.05). 3) The results of meat-related gene expression showed that the highest mRNA relative expression level of myostatin (MSTN) gene in group A₂, which was significantly different from the other three groups (P<0.05). The tropomyosin (TM) gene relative expression level in group A₄ was the highest, followed by group A₃, and it in groups A₃ and A₄ was significantly higher than that in groups A₁ and A₂ (P<0.05). The mRNA relative expression levels of fatty acid binding protein (FABP) and liportein lipase (LPL) genes in group A₄ were the highest, which was significantly different from the other three groups (P<0.05). Therefore, from the point of meat rate, and muscle crude protein, total fatty acid and total amino acid contents, the nutritional value of the small size (0 to 10 g) Procambarus clarkii is higher. Considering the texture characteristics of muscle and the expression results of four meat-related genes, the larger size (30 to 50 g) Procambarus clarkii has better meat quality and taste.

Key words： rice field culture; size; Procambarus clarkii; nutritional components; muscle characteristics; meat-related genes

Cite this article

LUO Yating , LIU Yi , LIU Jinmeng , XIAO Tiaoyi , LIU Qiaolin . Analysis of Muscle Nutritional Components of Different Sizes of Procambarus clarkii Cultured in Rice Field in Hunan Province[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4592 -4603 . DOI: 10.3969/j.issn.1006-267x.2021.08.039

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