MOLECULAR AND CELLULAR NUTRITION

Effects of Selenomethionine on Gene Differential Expression in Testis of Breeding Cocks and Function Prediction

  • HENG Nuo ,
  • MA Xin ,
  • GUO Yong ,
  • QI Xiaolong ,
  • SHENG Xihui ,
  • WANG Xiangguo ,
  • XING Kai ,
  • XIAO Longfei ,
  • CHEN Yu ,
  • WANG Liang ,
  • NI Hemin
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  • 1. College of Animal Science and Technology, Beijing Agricultural University, Beijing 102206, China;
    2. Beijing General Station of Animal Husbandry, Beijing 100107, China

Received date: 2021-01-24

  Online published: 2021-08-11

Supported by

 

Abstract

In this experiment, breeding cocks in the late breeding period were used as animal models to compare the gene differential expression in testis tissues between the control group and the organic selenium group. Combined with GO and KEGG functional enrichment analysis, the aim of this study was to explore the effects of selenium on reproductive performance of breeding cocks, and to provide theoretical support for nutritional regulation to improve reproductive performance of breeding cocks in the future. A total of 120 healthy Loman brown breeding cocks with the same body condition at the age of 378 days were randomly divided into 2 groups with 6 replicates per group and 10 cocks per replicate. Cocks in the control group were fed a basal diet, and those in the experimental group were fed the basal diet supplemented with selenomethionine at a selenium level of 1 mg/kg. The pre-trial period lasted for 1 week and the experimental period lasted for 4 weeks. At the end of the experiment period, one of the cocks from each replicate was randomly selected to take the left testis. The testis transcriptome was sequenced to screen significantly expressed genes in the testis tissues. The results showed that a total of 111 differentially expressed genes, including 55 up-regulated genes and 56 down-regulated genes, were found by transcriptome sequencing in testis tissue samples of cocks from two groups, and the number of annotated genes was 86. Among the differentially expressed genes, tubulin alpha-8 chain (TUBA8A) gene had the highest up-regulated differential expression, while calcium/calmodulin-dependent protein kinase Ⅳ like (CAMK4L) had the highest down-regulated differential expression. The expression trend of RT-PCR was similar to that of transcriptome sequencing in testis tissues. GO function analysis showed that protein chibby homology 1 (CBY1), TUBA8A, ADP-ribosyltransferase 4 (ART4), calcyclin binding protein (CACYBP), adenylate kinase 7 (AK7) and mitochondrial ribosomal protein L17 (MRPL17) genes were involved in cellular processes, biological regulation, metabolic processes, cellular components and molecular function to improve the reproductive performance of breeding cocks, and there can be used as candidate genes to study the reproductive performance of cocks for further study and discussion.

Cite this article

HENG Nuo , MA Xin , GUO Yong , QI Xiaolong , SHENG Xihui , WANG Xiangguo , XING Kai , XIAO Longfei , CHEN Yu , WANG Liang , NI Hemin . Effects of Selenomethionine on Gene Differential Expression in Testis of Breeding Cocks and Function Prediction[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4626 -4636 . DOI: 10.3969/j.issn.1006-267x.2021.08.042

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