Mitigative Effects of Melatonin on Lipopolysaccharide-Induced Inflammation in Bovine Mammary Epithelial Cells

LI Hongyang; NAN Xuemei; SUN Peng

doi:10.3969/j.issn.1006-267x.2021.08.043

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 8: 4637 - 4644

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.08.043

MOLECULAR AND CELLULAR NUTRITION

Mitigative Effects of Melatonin on Lipopolysaccharide-Induced Inflammation in Bovine Mammary Epithelial Cells

LI Hongyang ,
NAN Xuemei ,
SUN Peng

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State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2021-01-11

Online published: 2021-08-11

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Abstract

The purpose of this study was to investigate the mitigative effects and underlying mechanisms of melatonin (MT) on lipopolysaccharide (LPS)-induced inflammation in bovine mammary epithelial cells (BMECs). The BMECs were treated with different concentrations (0.1, 0.5, 1.0, 5.0 and 10.0 μg/mL) of LPS for 6 and 12 hours, respectively. The BMECs viability was detected and the inflammatory cytokines[tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6)] contents was measured, and the BMECs model was established with 12 hours and 10 μg/mL LPS for formal experiment. The formal experiment divided into 7 groups with 4 replicates in each group. BMECs in the control group without LPS-induced and MT treated, BMECs in the LPS group were LPS-induced 12 hours, and BMECs in LPS+MT groups were LPS-induced 12 hours and treated with different concentrations (1, 5, 10, 50 and 100 μmol/L) of LPS for 48 hours, respectively. The results showed as follows:1) compared with the control group, the BMECs TNF-α content of LPS group was increased (P>0.05), and the contents of IL-6 and IL-1β in BMECs were significantly increased (P<0.05). Compared with the LPS group, the BMECs TNF-α content of 10 and 50 μmol/L MT groups was significantly decreased (P<0.05), the BMECs IL-6 content of 1, 5, 10 and 100 μmol/L MT groups was significantly decreased (P<0.05), and the BMECs IL-1β content of 10 μmol/L MT group was significantly decreased (P<0.05). 2) Compared with the control group, the BMECs Toll-like receptor 4 (TLR4) protein expression level of LPS group was increased (P>0.05), and the protein expression levels of nuclear factor-κB homologous protein (P65) and nuclear factor-κB inhibitory protein-α (IκB-α) in BMECs were significantly decreased (P<0.05). Compared with the LPS group, the BMECs TLR4 protein expression level of 10 and 100 μmol/L MT groups was significantly decreased (P<0.05), the BMECs P65 protein expression level of 1, 10 and 50 μmol/L MT groups was significantly increased (P<0.05 or P<0.01), and the BMECs IκB-α protein expression level of 1, 5, 10 and 50 μmol/L MT group was significantly increased (P<0.05 or P<0.01). It is concluded that MT can reduce the contents of TNF-α, IL-1β and IL-6 in BMECs induced by LPS, regulate the protein expression levels of TLR4, P65 and IκB-α in BMECs induced by LPS, and likely participate nuclear factor-κB inflammatory pathway, which exerted anti-inflammatory effect in LPS-challenged BMECs.

Key words： mastitis; melatonin; lipopolysaccharide; anti-inflammatory; inflammatory pathway

Cite this article

LI Hongyang , NAN Xuemei , SUN Peng . Mitigative Effects of Melatonin on Lipopolysaccharide-Induced Inflammation in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4637 -4644 . DOI: 10.3969/j.issn.1006-267x.2021.08.043

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