EXPERIMENTAL METHOD AND ANIMAL

A Method for Simultaneous Determination of Aflatoxin B1, Zearalenone and Deoxynivalenol in Feed by High Performance Liquid Chromatography

  • LIU Ying ,
  • JIN Yongpeng ,
  • LUO Sunlin ,
  • CHEN Yiqiang
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  • State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

Received date: 2021-01-18

  Online published: 2021-08-11

Supported by

 

Abstract

A high performance liquid chromatographic (HPLC) method was developed for simultaneous determination of aflatoxin B1, zearalenone and deoxynivalenol in feed. Samples were extracted with acetonitrile-water, after vortexing and high-speed centrifugation, 2 mL of supernatant was transferred and mixed with 28 mL phosphate buffer saline (PBS, pH 7.0) containing 1% tween-20. Then the extracted solution was purified with immune-affinity column and eluted with 2 mL of methanol. The eluent was blow-dried by concentrated nitrogen at 50℃ and then redissolved in 50% methanol-water. Finally, the reconstituted solution was detected by a high performance liquid chromatography coupled with a photochemical derivatizer, a fluorescence detector and an ultraviolet detector. The results showed that the calibration curves of aflatoxin B1 were linear in 2.5 to 50.0 ng/mL, the calibration curves of zearalenone were linear in 50 to 2 500 ng/mL, and the calibration curves of deoxynivalenol were linear in 250 to 5 000 ng/mL. The average recovery ratio was ranged from of 81.2% to 92.3%, and the relative standard deviation (RSD) was ranged from 4.3% to 9.5%. The established method was then used to determine the contents of aflatoxin B1, zearalenone and deoxynivalenol in four kinds of feed samples, it was found that all the detected samples contained more than one mycotoxin, implying that the feed samples were widely contaminated with mycotoxins. In conclusion, the established method has high accuracy, precision and good stability, and it can be used as a simultaneous detection method for aflatoxin B1, zearalenone and deoxynivalenol in feed.

Cite this article

LIU Ying , JIN Yongpeng , LUO Sunlin , CHEN Yiqiang . A Method for Simultaneous Determination of Aflatoxin B1, Zearalenone and Deoxynivalenol in Feed by High Performance Liquid Chromatography[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4684 -4693 . DOI: 10.3969/j.issn.1006-267x.2021.08.047

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