EXPERIMENTAL METHOD AND ANIMAL

Effects of Portulaca oleracea L. Polysaccharide on Intestinal Flora in Weaned Rats

  • HUANG Xiaoliu ,
  • LUO Hui ,
  • HUANG Yushan ,
  • XU Dongfeng ,
  • DENG Xianqing ,
  • WANG Weiye
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  • 1. School of Medicine, Jinggangshan University, Ji'an 343009, China;
    2. Key Laboratory of Agricultural Environmental Pollution Prevention and Control in Red Soil Hilly Region of Jiangxi Province, Jinggangshan University, Ji'an 343009, China;
    3. School of Life Sciences, Jinggangshan University, Ji'an 343009, China

Received date: 2021-01-14

  Online published: 2021-08-11

Supported by

 

Abstract

This study was to investigate the effects of Portulaca oleracea L. polysaccharide (POP) on the changes of intestinal flora in weaned rats. Thirty weaned SD rats[(21±1) days of age] were randomly divided into three groups with ten rats in each group. The experimental animals were fed the basal diet, and rats in the low-dose group (LD group) and high-dose group (HD group) of POP were supplemented with 100 and 200 mg/kg BW POP (dissolved in 2 mL normal saline) respectively, while control group (Con group) were supplemented with 2 mL normal saline once a day for 28 consecutive days. The sequences of V3 to V4 regions of 16S rRNA were performed on the collected faeces using Illumina MiSeq platform, and the analysis of alpha-diversity and beta-diversity were conducted using QIIME, followed RDP and NCBI-16S databases were used for species annotation. The results showed that there were 4 725 identical operational taxonomic units (OTUs) in the 3 groups, Con group had 140 characteristic OTUs, LD group had 367 characteristic OTUs, and HD group had 174 characteristic OTUs. The intestinal flora of each group had similar alpha-diversity, but beta-diversity was significantly differences between groups (P<0.05). Compared with Con group, the relative abundances of Bifidobacterium in HD group and Lactobacillales in LD group were significantly increased (P<0.05), respectively. The Porphyromonadaceae-unclassified and Alloprevotella were the dominant species in the Con group, and the metabolisms of nucleotide, vitamins, lipids, metabolism and biosynthesis of carbohydrate in Con group reduced. In conclusion, POP not only changes the structure of intestinal flora in weaned rats, but also facilitates the colonization of probiotics such as Lactobacillus and Bifidobacterium and affects the metabolic function of the body, and is a potential dietary additive for young animals.

Cite this article

HUANG Xiaoliu , LUO Hui , HUANG Yushan , XU Dongfeng , DENG Xianqing , WANG Weiye . Effects of Portulaca oleracea L. Polysaccharide on Intestinal Flora in Weaned Rats[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4694 -4707 . DOI: 10.3969/j.issn.1006-267x.2021.08.048

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