EXPERIMENTAL METHOD AND ANIMAL

Effects of D-Aspartic on Antioxidant and Inflammation of Dextran Sodium Sulfate Induced Colitis of Mice

  • HU Xian ,
  • WANG Chenyu ,
  • LIU Di ,
  • LONG Jing ,
  • FAN Wenjun ,
  • HE Liuqin ,
  • LI Tiejun ,
  • YAO Jiming
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  • 1. Hunan Province Key Laboratory of Animal Intestinal Function and Regulation, College of Life Sciences, Hunan Normal University, Changsha 410081, China;
    2. Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, National Engineering Laboratory of Poultry Breeding Pollution Control and Resource Technology, Institute of Subtropical Agricultural Ecology, Chinese Academy of Sciences, Changsha 410125, China;
    3. University of Chinese Academy of Sciences, Beijing 100049, China;
    4. Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;
    5. Guangdong Wangda Group Co., Ltd., Guangzhou 510663, China

Received date: 2021-01-29

  Online published: 2021-08-11

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Abstract

This experiment was conducted to study the repairing effects of D-aspartic acid (D-Asp) on dextran sodium sulfate (DSS) induced colonic intestinal injury of mice. A total of 40 C57BL/6J male mice with similar body weight were randomly divided into 5 groups, which were the control group, 0 mmol/L D-Asp group, 0.15 mmol/L D-Asp group, 2.30 mmol/L D-Asp group and 7.70 mmol/L D-Asp group, each group contained 8 replicates and each replicate contained 1 mouse, the mice were reared in a single cage. During days 1 to 7, the mice in the control group drank sterile water, while the others in other groups drank sterile water containing 3.5% DSS, to induce colitis of mice. From day 8, the control group and 0 mmol/L D-Asp group were rectal perfused with 0.1 mL normal saline, and the mice in the other groups were rectal perfused with 0.1 mL D-Asp at the concentrations of 0.15, 2.30 and 7.70 mmol/L, respectively, and perfused once every 1 d for 5 times. The results showed as follows:1) compared with the control group, the body weight on day 5 and average daily feed intake of 0 mmol/L D-Asp group were significantly decreased (P<0.05), and the colon length was shortened and the phenomenon of blood infiltration occurred. Compared with the 0 mmol/L D-Asp group, the body weight on days 12 to 14 and average daily feed intake of 7.70 mmol/L D-Asp group were significantly increased (P<0.05), the colon became longer, the villi height and crypt depth became shallower, and the arrangement of goblet cells was more regular. 2) Compared with the 0 mmol/L D-Asp group, the serum interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) contents and eosinophil peroxidase (EPO) activity of 7.70 mmol/L D-Asp group were significantly decreased (P<0.05), the mRNA relative expression level of aspartate/glutamate carrier 2 (SLC25A13) in colon was significantly increased (P<0.05), and the mRNA relative expression levels of interleukin-6 (IL-6), acetyl CoA carboxylase (ACC) and aspartate/glutamate carrier 1 (SLC25A12) in colon were significantly decreased (P<0.05). In conclusion, rectal perfused D-Asp can inhibit the secretion of inflammatory cytokines and improve the morphological structure of colon by increasing the activity of antioxidant enzymes and the expression of related genes, thereby promoting the repair of mouse colitis damage caused by DSS.

Cite this article

HU Xian , WANG Chenyu , LIU Di , LONG Jing , FAN Wenjun , HE Liuqin , LI Tiejun , YAO Jiming . Effects of D-Aspartic on Antioxidant and Inflammation of Dextran Sodium Sulfate Induced Colitis of Mice[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4708 -4718 . DOI: 10.3969/j.issn.1006-267x.2021.08.049

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