EXPERIMENTAL METHOD AND ANIMAL

Effects of Staphylococcus haemolyticus-Derived Extracellular Vesicles on Growth Performance, Immunity, Intestinal Morphology and Microbiota of Mice

  • LUO Meiying ,
  • LING Mingwang ,
  • ZHAN Zhouling ,
  • ZHENG Jianghong ,
  • ZHONG Xiaotong ,
  • OU Chunxiu ,
  • LIANG Yingqian ,
  • FENG Xin ,
  • WANG Ruixiao ,
  • HUANG Dechun ,
  • ZHANG Huihua ,
  • QI Qien
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  • 1. School of Life Science and Engineering, Foshan University, Foshan 528231, China;
    2. Guangdong Province Key Laboratory of Animal Nutritional Regulation, South China Agricultural University, Guangzhou 510642, China

Received date: 2021-04-21

  Online published: 2021-08-11

Supported by

 

Abstract

This experiment was conducted to study the effects of Staphylococcus haemolyticus-derived extracellular vesicles (SH-EVs) on growth performance, immunity, intestinal morphology and microflora of mice. Twenty healthy male C57BL/6 mice with similar body weight and age were randomly assigned into 2 groups with 10 replicates per group and 1 mouse per replicate. Mice in control group were fed 150 μL phosphate buffer solution (PBS), and those in test group were fed 150 μL SH-EVs suspensions (containing 500 μg SH-EVs) every other day. The experiment lasted for 5 days. The results showed as follows:1) compared with the control group, administration of SH-EVs significantly decreased the average daily feed intake (ADFI) and average daily gain (ADG) of mice (P<0.05), and tended to increase the ratio of feed to gain (F/G) (P=0.052). 2) Compared with the control group, administration of SH-EVs significantly increased the serum tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) contents (P<0.05), significantly decreased serum immunoglobulin A (IgA) content (P<0.05), and tended to decrease serum immunoglobulin G (IgG) content (P=0.067). 3) Administration of SH-EVs resulted in ileum villi injury and sparse of mice. 4) Compared with the control group, administration of SH-EVs significantly reduced the relative abundance of Bacteroidetes in intestine of mice (P<0.05), and significantly increased the relative abundances of Acidobacteria, Chloroflexi, Proteobacteria and Planctomycetes at phylum level (P<0.05); significantly reduced the relative abundances of Prevotellaceae_UCG-001, Bacteroides and Alloprevotella in intestine of mice at genus level (P<0.05); significantly reduced the relative abundance of Bacteroides acidifaciens in intestine of mice (P<0.05), and significantly increased the relative abundance of Lachnospiraceae_bacterium_COE1 at species level (P<0.05). In conclusion, administration of SH-EVs reduces the growth performance and immunity of mice, damages the morphological development of intestine and disturbs the structure of intestinal microflora to a certain extent, and has adverse effects on the health of mice.

Cite this article

LUO Meiying , LING Mingwang , ZHAN Zhouling , ZHENG Jianghong , ZHONG Xiaotong , OU Chunxiu , LIANG Yingqian , FENG Xin , WANG Ruixiao , HUANG Dechun , ZHANG Huihua , QI Qien . Effects of Staphylococcus haemolyticus-Derived Extracellular Vesicles on Growth Performance, Immunity, Intestinal Morphology and Microbiota of Mice[J]. Chinese Journal of Animal Nutrition, 2021 , 33(8) : 4719 -4729 . DOI: 10.3969/j.issn.1006-267x.2021.08.050

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