SWINE NUTRITION AND FEED

Effects of Methyl-Donor Supplementation in Diets for Pregnant Sows on Hepatic Glycolipid Metabolism and Mitochondrial Function of Newborn Piglets

  • JIAN Li ,
  • ZOU Tiande ,
  • LI Jianjun ,
  • CHEN Jun ,
  • HE Qin ,
  • HE Jia ,
  • XIA Yingying ,
  • XIE Fei ,
  • WANG Zirui ,
  • YOU Jinming
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  • 1. Jiangxi Province Key Laboratory of Animal Nutrition, Jiangxi Province Nutrition & Feed Development Engineering Research Center, Jiangxi Province Key Innovation Center of Integration in Production and Education for High-Quality and Safe Livestock and Poultry, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;
    2. Ganzhou Institute of Animal Husbandry, Ganzhou 341000, China

Received date: 2021-01-31

  Online published: 2021-09-18

Supported by

 

Abstract

This study aimed to investigate the effects of methyl-donor supplementation in diets for pregnant sows on hepatic glycolipid metabolism and mitochondrial function of newborn piglets. "Duroc×Erhuanlian" crossbred gilts with the body weight of (102.8±6.3) kg were randomly allotted two groups after artificial insemination (21 and 22 gilts for control group and methyl-donor group, respectively). Gilts in the methyl-donor group and control group were fed a basal diet supplemented with or without methyl-donor, and the methyl-donor included 4 700 mg/kg methionine, 16.3 mg/kg folate, 2 230 mg/kg choline, 0.15 mg/kg vitamin B12 and 1 180 mg/kg vitamin B6. The feeding trail were conducted until farrowing. After farrowing, the newborn piglets were weighted, and 8 litters were selected and 1 neonate per litter were slaughtered for liver samples collection. The visceral organs of newborns were separated and weighed, and liver samples were collected for the analysis of glycolipid metabolism and mitochondrial function indices. The results show as follows:1) compared with the control group, methyl-donor supplementation in the pregnant sows' diet did not significantly affect the individual weight and liver weight of newborn piglets (P>0.05), but significantly increased the spleen weight of newborn piglets (P<0.05). 2) Compared with the control group, methyl-donor supplementation in the pregnant sows' diet significantly increased the hepatic glycogen content, phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6PC) activities of newborn piglets (P<0.05), and significantly decreased the hepatic triglyceride and total cholesterol contents of newborn piglets (P<0.05). 3) Compared with the control group, methyl-donor supplementation in the pregnant sows' diet significantly down-regulated the mRNA and protein relative expression levels of hepatic lipid-synthesis-related genes such as sterol regulatory element-binding protein-1C (SREBP-1C) and stearoyl coenzyme A desaturase (SCD) (P<0.05), and significantly up-regulated hepatic gluconeogenesis-related enzymes such as PEPCK1 and G6PC at both mRNA and protein relative expression levels, as well as the mRNA relative expression levels of hepatic lipidolysis-related genes such as peroxisome proliferator-activated receptor-α (PPARα) and lipoprotein lipase (LPL) of newborn piglets (P<0.05). 4) Compared with the control group, methyl-donor supplementation in the pregnant sows' diet significantly increased the contents of adenosine triphosphate (ATP), NAD+ and mitochondrial DNA (mtDNA), and the activities of citrate synthase (CS) and succinate dehydrogenase (SDH) in the liver of newborn piglets (P<0.05). 5) Compared with the control group, methyl-donor supplementation in the pregnant sows' diet significantly up-regulated the mRNA relative expression levels of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) and nuclear respiratory factor-1 (NRF-1) in liver of newborn piglets (P<0.05). Overall, methyl-donor supplementation in the pregnant sows' diet can inhibit the hepatic lipid synthesis, promote the gluconeogenesis, and thus improve the hepatic glycolipid metabolism and mitochondrial function of newborn piglets.

Cite this article

JIAN Li , ZOU Tiande , LI Jianjun , CHEN Jun , HE Qin , HE Jia , XIA Yingying , XIE Fei , WANG Zirui , YOU Jinming . Effects of Methyl-Donor Supplementation in Diets for Pregnant Sows on Hepatic Glycolipid Metabolism and Mitochondrial Function of Newborn Piglets[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 4920 -4930 . DOI: 10.3969/j.issn.1006-267x.2021.09.013

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