SWINE NUTRITION AND FEED

Effects of Dietary Betaine Hydrochloride Supplementation on Fecal Microbiota Composition and Metabolites Contents of Sows During Pregnancy and Lactation

  • MA Cui ,
  • GAO Qiankun ,
  • ZHU Qian ,
  • SONG Mingtong ,
  • GENG Meimei ,
  • HAN Qiang ,
  • KONG Xiangfeng
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  • 1. Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. Sunwin Biotech Shandong Co., Ltd., Weifang 261000, China

Received date: 2021-02-24

  Online published: 2021-09-18

Supported by

 

Abstract

This experiment was conducted to study the effects of dietary betaine hydrochloride supplementation on fecal microbiota composition and metabolites contents of sows during pregnancy and lactation. Forty pregnant Bama mini-pigs with three to seven parities were used and randomly allocated to two groups with 20 pigs per group, and fed individually. Sows in the control group were fed a basal diet and others in the experiment group were fed the basal diet supplemented with 3.5 kg/t betaine hydrochloride. The trial period was from day 3 after mating to postpartum day 21. At day 105 of pregnancy and day 21 of lactation, the fresh fecal samples of sows were collected for microbial 16S rDNA sequencing, as well as the determination of short-chain fatty acids, bioamines, indole and skatole contents. The results showed that compared with the control group, dietary betaine hydrochloride supplementation significantly increased the fecal Elusimicrobia and Coprobacillus relative abundances and cadaverine content at day 105 of pregnancy (P<0.05), and significantly increased the fecal microbiota Chao1, ACE indexes, fecal Anaeroplasma relative abundance and putrescine, spermidine contents at day 21 of lactation (P<0.05). The Pearson correlation analysis found that the fecal Anaeroplasma relative abundance was positively correlated with spermidine content (P<0.05), and was negatively correlated with isobutyrate and isovalerate contents (P<0.05). The KEGG pathway analysis showed that compared with the control group, dietary betaine hydrochloride supplementation up-regulated the nucleotide metabolism, glycan biosynthesis and metabolism and enzyme families pathways at day 105 of pregnancy, as well as xenobiotics biodegradation and metabolism, metabolism of terpenoids and polyketides, metabolism of other amino acids and lipid metabolism pathways at day 21 of lactation. In conclusion, dietary betaine hydrochloride supplementation can alter fecal microbiota composition and metabolites contents of sows during pregnancy and lactation, and then improve the host's metabolism.

Cite this article

MA Cui , GAO Qiankun , ZHU Qian , SONG Mingtong , GENG Meimei , HAN Qiang , KONG Xiangfeng . Effects of Dietary Betaine Hydrochloride Supplementation on Fecal Microbiota Composition and Metabolites Contents of Sows During Pregnancy and Lactation[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 4931 -4942 . DOI: 10.3969/j.issn.1006-267x.2021.09.014

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