POULTRY NUTRITION AND FEED

Effects of Bacillus subtilis on Performance, Egg Quality, Intestinal Morphology and Cecal Microbiota of Laying Hens during Late Laying Stage

  • MA Wenfeng ,
  • LIAO Jiahui ,
  • CHEN Xiaochen ,
  • WANG Zhanbin ,
  • WU Qiujue ,
  • JIAO Guobao ,
  • CHEN Xiaoyu
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  • 1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China;
    2. Henan Xinyangshao Biochemical Technology Co., Ltd., Sanmenxia 472400, China

Received date: 2021-01-31

  Online published: 2021-09-18

Supported by

 

Abstract

This experiment was conducted to study the effects of Bacillus subtilis on performance, egg quality, intestinal morphology and cecal microbiota of laying hens during late laying stage. A total of 240 Hy-Line brown laying hens aged at 60 weeks were randomly divided into 2 groups with 8 replicates per group and 15 hens per replicate. Laying hens in the control group were fed a basal diet, and others in the experimental group were fed the basal diet supplemented with 0.03% Bacillus subtilis (2×106 CFU/g). The preliminary period lasted for 10 days and the experiment lasted for 60 days. The results showed as follows:1) there were no significant differences in the laying rate, average daily egg weight, feed to gain ratio and average daily feed intake between 2 groups (P>0.05). 2) Compared with the control group, the albumen height and Haugh unit of the experimental group were significantly increased (P<0.05). 3) Compared with the control group, the villus height in jejunum and ileum of the experimental group was significantly increased (P<0.05). 4) Compared with the control group, the relative abundances of Tenericutes and Elusimicrobia in cecum of the experimental group were significantly decreased (P<0.05), and the relative abundance of Lactobacillus in cecum was significantly decreased (P<0.05). In conclusion, the supplementation of 0.03% Bacillus subtilis in diet can improve intestinal morphology and egg quality of laying hens during late laying stage, affect the cecal microbiota composition, but has no significant effect on performance.

Cite this article

MA Wenfeng , LIAO Jiahui , CHEN Xiaochen , WANG Zhanbin , WU Qiujue , JIAO Guobao , CHEN Xiaoyu . Effects of Bacillus subtilis on Performance, Egg Quality, Intestinal Morphology and Cecal Microbiota of Laying Hens during Late Laying Stage[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 4958 -4965 . DOI: 10.3969/j.issn.1006-267x.2021.09.016

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