RUMINANT AND HERBIVORE NUTRITION AND FEED

Evaluation of Biological Safety of Selenium Yeast for Tan Sheep: Growth Performance, Blood Routine Parameters, Selenoprotein Gene Expression and Enrichment Regularity

  • JIA Xueting ,
  • GUO Xiaoqing ,
  • HAN Yunsheng ,
  • LI Jing ,
  • ZHAO Qingyu ,
  • ZHANG Kai ,
  • TANG Chaohua ,
  • MA Qing ,
  • WANG Jin ,
  • ZHAO Zhengwei ,
  • ZHANG Junmin ,
  • QIN Yuchang
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  • 1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China;
    4. Institute of Animal Science, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750002, China

Received date: 2021-02-23

  Online published: 2021-09-18

Supported by

 

Abstract

This study was conducted to evaluate the effects of dietary selenium yeast supplemental level on growth performance, blood routine parameters, selenoprotein gene expression and tissue and organ selenium contents of Tan sheep, and to evaluate the biological safety of selenium yeast for Tan sheep and reveal the biological enrichment regularity of selenium. Sixty-four healthy male Tan sheep with similar body weight[(32±2) kg] were selected and randomly assigned into 4 groups with 16 replicates in each group and 1 sheep in each replicate. The content of selenium in corn-soybean meal basal diet was 0.16 mg/kg, and 28.94, 105.46, 258.51 and 564.60 mg/kg selenium yeast were added to the basal diet, to make the contents of selenium in diets were 0.25 (group Ⅰ), 0.50 (group Ⅱ), 1.00 (group Ⅲ) and 2.00 (group Ⅳ) mg/kg, respectively. The pre-experimental period lasted for 10 days, and the experimental period lasted for 60 days. The results showed as follows:1) dietary selenium yeast supplemental level had no significant effects on growth performance of Tan sheep (P>0.05). 2) Compared with group Ⅰ, the mean corpuscular volume and eosinophil count in blood of Tan sheep of group Ⅲ were significantly increased (P<0.05). Compared with group Ⅱ, the standard deviation of red cell volume distribution width in blood of Tan sheep of group Ⅳ was significantly decreased (P<0.05). 3) Compared with group Ⅰ, the mRNA relative expression levels of glutathione peroxidase 1 (GPX1) and glutathione peroxidase 3 (GPX3) in liver of Tan sheep of group Ⅲ were significantly increased in a quadratic manner (P<0.01). Compared with group Ⅰ, the mRNA relative expression level of thioredoxin reductase 1 (TXNRD1) in liver of Tan sheep of groups Ⅱ, Ⅲ and Ⅳ was significantly decreased in a quadratic manner (P<0.05), and the mRNA relative expression level of thyroxine deiodinase 1 (DIO1) was significantly decreased in a linear manner (P<0.05). There were no significant differences in mRNA relative expression levels of selenoprotein in muscle among all groups (P>0.05). 4) The serum selenium content of group Ⅳ was significantly higher than that of groups Ⅰ, Ⅱ and Ⅲ (P<0.01). With dietary selenium yeast supplemental level increased, the contents of selenium in dorsal muscle, liver, kidney, lung, heart, pancreas and duodenum were significantly increased in a quadratic manner (P<0.01), and the contents of selenium in spleen and testis were significantly increased in a linear manner (P<0.01). In summary, use selenium yeast as a supplement source, dietary selenium content of 0.25 to 2.00 mg/kg has no adverse effects on the growth performance, blood routine parameters, selenoprotein gene expression and tissue selenium contents of Tan sheep; and it will be safe for Tan sheep when dietary selenium content get 2.00 mg/kg. The selenium enrichment in serum and tissue and organ of Tan sheep increases with dietary selenium yeast supplemental level increase.

Cite this article

JIA Xueting , GUO Xiaoqing , HAN Yunsheng , LI Jing , ZHAO Qingyu , ZHANG Kai , TANG Chaohua , MA Qing , WANG Jin , ZHAO Zhengwei , ZHANG Junmin , QIN Yuchang . Evaluation of Biological Safety of Selenium Yeast for Tan Sheep: Growth Performance, Blood Routine Parameters, Selenoprotein Gene Expression and Enrichment Regularity[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 5086 -5097 . DOI: 10.3969/j.issn.1006-267x.2021.09.029

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