Effects of Supplementary Feeding Guanidinoacetic Acid on Movement Performance, Glucose Metabolism and Antioxidant Capacity of Yili Horses

LIU Lyu; LI Xiaobin; MA Yan; YANG Hao; ZHANG Wenjie; LU Hao; WEI Xinlan; GAO Junjie; OUYANG Wen; YANG Kailun

doi:10.3969/j.issn.1006-267x.2021.09.036

2021 , Vol. 33 >Issue 9: 5162 - 5173

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.09.036

RUMINANT AND HERBIVORE NUTRITION AND FEED

Effects of Supplementary Feeding Guanidinoacetic Acid on Movement Performance, Glucose Metabolism and Antioxidant Capacity of Yili Horses

LIU Lyu ,
LI Xiaobin ,
MA Yan ,
YANG Hao ,
ZHANG Wenjie ,
LU Hao ,
WEI Xinlan ,
GAO Junjie ,
OUYANG Wen ,
YANG Kailun

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1. Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Xinjiang Agricultural University, Urumqi 830052, China;
2. Yili Kazak Autonomous Prefecture of Zhaosu Racecourse, Zhaosu 835000, China

Received date: 2021-02-25

Online published: 2021-09-18

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Abstract

The purpose of this study was to investigate the effects of supplementary feeding guanidineacetic acid (GAA) on movement performance, glucose metabolism and antioxidant capacity of Yili horses. Twenty Yili horses aged 2 to 3 years old, similar in weight (373.20±30.53) kg, and rigorously trained for speed races, were randomly divided into 4 groups and with 5 horses (3♀+2) in each group, and the training intensity were consistent. Each horse was fed 4 kg of concentrate supplements per day, and freely collected dry herbage. On this basis, each horse in each group was fed 0 (CON group), 25 (25 GAA group), 50 (50 GAA group) and 75 mg/kg GAA (75 GAA group) every day for an 8-week trial. On the 47th day of the trial, a 2 000 m speed race was performed, and blood and urine samples were collected. The results showed as follows:1) supplementation of GAA acid can shorten the time of 2 000 m race in Yili horses. 2) The contents of plasma GAA, creatine and creatinine in 50 GAA group were significantly higher than those in CON group immediately after competition (P<0.05). Plasma creatine kinase activity in 50 GAA and 75 GAA groups was significantly higher than that in CON group immediately after competition (P<0.01). The urine creatinine excretion in all experimental groups was significantly higher than that in CON group (P<0.01). 3) The plasma insulin content in each experimental group was significantly higher than that in CON group at 2 h before, immediately and 2 h after competition (P<0.05). The contents of plasma glucose and lactic acid in each experimental group at 2 h before, immediately and 2 h after the competition were significantly lower than those in the CON group (P<0.05). 4) The activities of plasma total antioxidant capacity, glutathione peroxidase and superoxide dismutase in all experimental groups were significantly higher than those in CON group at 2 h before and 2 h after competition (P<0.05). Under the conditions of this experiment, GAA supplementation increase the content of plasma creatine metabolites, the ability of antioxidation and the performance of 2000 m speed race, and promote the energy supply of glucose metabolism to exercise. Therefore, GAA can be used as a nutritional regulator to relieve exercise fatigue or promote fatigue recovery of sports horses. The best supplementation level of GAA is 50 mg/kg per horse.

Key words： Yili horses; GAA; creatine; glucose metabolism; antioxidant capacity

Cite this article

LIU Lyu , LI Xiaobin , MA Yan , YANG Hao , ZHANG Wenjie , LU Hao , WEI Xinlan , GAO Junjie , OUYANG Wen , YANG Kailun . Effects of Supplementary Feeding Guanidinoacetic Acid on Movement Performance, Glucose Metabolism and Antioxidant Capacity of Yili Horses[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 5162 -5173 . DOI: 10.3969/j.issn.1006-267x.2021.09.036

References

[1] LESIMPLE C,REVERCHON-BILLOT L,GALLOUX P,et al.Free movement:a key for welfare improvement in sport horses?[J].Applied Animal Behaviour Science,2020,225:104972.
[2] SAUER F J,HERMANN M,RAMSEYER A,et al.Effects of breed, management and personality on cortisol reactivity in sport horses[J].PLoS One,2019,14(12):e0221794.
[3] 王誉杰,张进威,王讯,等.胍基乙酸及代谢产物肌酸的研究进展[J].畜牧兽医学报,2018,49(8):1577-1584. WANG Y J,ZHANG J W,WANG X,et al.The research progress of guanidinoacetic acid and creatine[J].Acta Veterinaria Et Zootechnica Sinica,2018,49(8):1577-1584.(in Chinese)
[4] MICHIELS J,MAERTENS L,BUYSE J,et al.Supplementation of guanidinoacetic acid to broiler diets:effects on performance, carcass characteristics,meat quality,and energy metabolism[J].Poultry Science,2012,91(2):402-412.

[5] DILGER R N,BRYANT-ANGELONI K,PAYNE R L,et al.Dietary guanidino acetic acid is an efficacious replacement for arginine for young chicks[J].Poultry Science,2013,92(1):171-177.

[6] GUIDI C,POTENZA L,SESTILI P,et al.Differential effect of creatine on oxidatively-injured mitochondrial and nuclear DNA[J].Biochimica et Biophysica Acta,2008,1780(1):16-26.

[7] OSTOJIC S M,STOJANOVIC M D,HOFFMAN J R.Six-week oral guanidinoacetic acid administration improves muscular performance in healthy volunteers[J].Journal of Investigative Medicine,2015,63(8):942-946.
[8] 李晓斌.补喂支链氨基酸、肌酸对运动马运动性能及血液生化指标的影响[D].硕士学位论文.乌鲁木齐:新疆农业大学,2014:34-48. LI X B.Effects of supplementation of branched chain amino acids and creatine on exercise performance and blood biochemical indexes of exercise horses[D].Master's Thesis.Urumqi:Xinjiang Agricultural University,2014:34-48.(in Chinese)
[9] ALE S S,AZARFAR A,GHEISARI A,et al.Energy utilisation of broiler chickens in response to guanidinoacetic acid supplementation in diets with various energy contents[J].The British Journal of Nutrition,2018,120(2):131-140.

[10] OSTOJIC S M,NIESS B,STOJANOVIC M D,et al.Serum creatine,creatinine and total homocysteine concentration-time profiles after a single oral dose of guanidinoacetic acid in humans[J].Journal of Functional Foods,2014,6:598-605.
[11] SETOUE M,OHUCHI S,MORITA T,et al.Hyperhomocysteinemia induced by guanidinoacetic acid is effectively suppressed by choline and betaine in rats[J].Bioscience Biotechnology and Biochemistry,2008,72(7):1696-1703.

[12] WADA T,SHIMBO H,OSAKA H.A simple screening method using ion chromatography for the diagnosis of cerebral creatine deficiency syndromes[J].Amino Acids,2012,43(2):993-997.

[13] PERSKY A M,BRAZEAU G A,HOCHHAUS G.Pharmacokinetics of the dietary supplement creatine[J].Clinical Pharmacokinetics,2003,42(6):557-574.

[14] 李洁蕾.胍基乙酸对骨骼肌细胞及保育猪的作用效果[D].硕士学位论文.北京:中国农业科学院,2017:36-45. LI J L.Effect of guanidine acetic acid on skeletal muscle cells and nursery pigs[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2017:36-45.(in Chinese)
[15] SOBOLL S,CONRAD A,EISTERT A,et al.Uptake of creatine phosphate into heart mitochondria:a leak in the creatine shuttle[J].Biochimica et Biophysica Acta,1997,1320(1):27-33.

[16] MCBREAIRTY L E,ROBINSON J L,FURLONG K R,et al.Guanidinoacetate is more effective than creatine at enhancing tissue creatine stores while consequently limiting methionine availability in Yucatan miniature pigs[J].PLoS One,2015,10(6):e0131563.
[17] ARDALAN M,BATISTA E D,TITGEMEYER E C.Effect of post-ruminal guanidinoacetic acid supplementation on creatine synthesis and plasma homocysteine concentrations in cattle[J].Journal of Animal Science,2020,98(3):skaa072.
[18] ARDALAN M,BATISTA E,ARMENDARIZ C,et al.Guanidinoacetic acid as a precursor of creatine for cattle[J].Kansas Agricultural Experiment Station Research Reports,2015,1(8):1-7.
[19] TACHIKAWA M,IKEDA S,FUJINAWA J,et al.γ-aminobutyric acid transporter 2 mediates the hepatic uptake of guanidinoacetate,the creatine biosynthetic precursor,in rats[J].PLoS One,2012,7(2):e32557.
[20] TAPEH R S,ZHANDI M,ZAGHARI M,et al.Effects of guanidinoacetic acid diet supplementation on semen quality and fertility of broiler breeder roosters[J].Theriogenology,2017,89:178-182.
[21] ABUDABOS A M,SALEH F,LEMME A,et al.The relationship between guanidino acetic acid and metabolisable energy level of diets on performance of broiler chickens[J].Italian Journal of Animal Science,2014,13(3):548-556.
[22] AZIZA A,MAHMOUD R,ZAHRAN E,et al.Dietary supplementation of guanidinoacetic acid improves growth, biochemical parameters, antioxidant capacity and cytokine responses in Nile tilapia (Oreochromis niloticus)[J].Fish & Shellfish Immunology,2020,97:367-374.
[23] HOUMARD J A,TYNDALL G L,MIDYETTE J B,et al.Effect of reduced training and training cessation on insulin action and muscle GLUT-4[J].Journal of Applied Physiology (Bethesda,Md.:1985),1996,81(3):1162-1168.

[24] 马亚妮,葛姗姗,熊正英,等.胰岛素与运动能力的关系[J].辽宁体育科技,2011,33(6):42-44. MA Y N,GE S S,XIONG Z Y,et al.The relationship of insulin and sports' ability[J].Liaoning Sport Science and Technology,2011,33(6):42-44.(in Chinese)
[25] MARCO J,CALLE C,HEDO J A,et al.Glucagon-releasing activity of guanidine compounds in mouse pancreatic islets[J].FEBS Letters,1976,64(1):52-54.

[26] OSTOJIC S M.Advanced physiological roles of guanidinoacetic acid[J].European Journal of Nutrition,2015,54(8):1211-1215.

[27] 孙正武.磷酸肌酸通过调节线粒体氧化磷酸化对抗脂多糖诱导的人脐静脉细胞凋亡[D].硕士学位论文.大连:大连医科大学,2014:36-41. SUN Z W.Phosphocreatine antagonizes apoptosis of human umbilical vein cells induced by lipopolysaccharide by regulating mitochondrial oxidative phosphorylation[D].Master's Thesis.Dalian:Dalian Medical University,2014:36-41.(in Chinese)
[28] MORI A,KOHNO M,MASUMIZU T,et al.Guanidino compounds generate reactive oxygen species[J].Biochemistry and Molecular Biology International,1996,40(1):135-143.
[29] PORPINO S,FERRAZ N,MONTEIRO M,et al.Cronic creatine supplementation and physical exercise reduces on oxidative stress in Wistar rats[J].BMC Proceedings,2014,8(Suppl.4):P9.
[30] 闻爱友,胡洪,吴学壮,等.日粮中添加胍基乙酸对断乳仔猪生长性能及抗氧化性能的影响[J].安徽科技学院学报,2020,34(3):6-9. WEN A Y,HU H,WU X Z,et al.Effects of guanidine acetic acid on growth performance and antioxidant capacity of weaned piglets[J].Journal of Anhui Science and Technology University,2020,34(3):6-9.(in Chinese)
[31] WANG L S,SHI B M,SHAN A S,et al.Effects of guanidinoacetic acid on growth performance,meat quality and antioxidation in growing-finishing pigs[J].Journal of Animal and Veterinary Advances,2012,5(11):631-636.
[32] KODAMBASHI EMAMI N,GOLIAN A,RHOADS D D,et al.Interactive effects of temperature and dietary supplementation of arginine or guanidinoacetic acid on nutritional and physiological responses in male broiler chickens[J].British Poultry Science,2017,58(1):87-94.

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