AQUATIC ANIMAL NUTRITION AND FEED

Effects of Dietary Berberine on Growth and Lipid Metabolism of Spotted Seabass (Lateolabrax maculatus)

  • WEI Yu ,
  • LU Kangle ,
  • WANG Ling ,
  • SONG Kai ,
  • ZHANG Chunxiao
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  • Xiamen Key Laboratory of Feed Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen 361061, China

Received date: 2021-02-22

  Online published: 2021-09-18

Abstract

This experiment was conducted to study the effects of berberine on growth and lipid metabolism of spotted seabass (Lateolabrax maculatus), and to explore whether berberine can be used as an additive to alleviate lipid deposition. Four diets were prepared:low-fat diet (LFD group), LFD+100 mg/kg berberine (LFD+BBR group), high-fat diet (HFD group) and HFD+100 mg/kg berberine (HFD+BBR group). A total of 360 fishes of similar size[(2.18±0.01) g] were stocked into 12 circular tanks (30 fishes per tank). Each diet was fed in three replicates, and the fish were fed for 8 weeks. After the breeding experiment, the final body weight and serum and liver samples were collected for biochemical analysis and gene expression analysis. The results showed that compared with LFD, HFD significantly increased weight gain rate, abdominal fat rate and serum total cholesterol and triglyceride contents (P<0.05). While the dietary berberine could significantly decrease the abdominal fat rate(P<0.05), and serum triglyceride content had a decreased trend. However, berberine significantly decreased the weight gain rate and protein efficiency rate (P<0.05), significantly increased the feed conversion ratio (P<0.05). Analysis of gene expression in liver cells found that HFD up-regulated the relative expression levels of genes regulated to apoptosis in liver (P<0.05), and significantly down-regulated the relative expression levels of genes related to lipolysis in hepatocytes (P<0.05). While the supplementation of berberine could significantly recover the alterations of these genes (P<0.05). The effects of HFD on these genes could be recovered by adding berberine to the diet. In addition, berberine supplementation could significantly increase the expression of apoptosis and autophagy related genes in adipocytes (P<0.05). In conclusion, HFD can cause excessive fat deposition and damage liver cells of Lateolabrax maculatus. Supplementation of berberine can alleviate fat deposition by promoting apoptosis and autophagy of adipocytes. Besides, berberine can also up-regulate the expression of lipogenic genes in liver, thus promoting lipogenesis. However, berberine can reduce the growth rate and feed conversion ratio of spotted seabass (Lateolabrax maculatus).

Cite this article

WEI Yu , LU Kangle , WANG Ling , SONG Kai , ZHANG Chunxiao . Effects of Dietary Berberine on Growth and Lipid Metabolism of Spotted Seabass (Lateolabrax maculatus)[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 5193 -5202 . DOI: 10.3969/j.issn.1006-267x.2021.09.039

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