MOLECULAR AND CELLULAR NUTRITION

Effects of Oligomeric Chitosan Selenium on Oxidative Damage and Endoplasmic Reticulum Stress in Zearalenon-Induced Pig Intestinal Epithelial Cells

  • FU Chunni ,
  • LI Yuanhui ,
  • LI Pengcheng ,
  • LI Liuan ,
  • QIN Shunyi
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  • Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received date: 2021-02-27

  Online published: 2021-09-18

Abstract

This study was to investigate the antagonistic effects of oligomeric chitosan selenium on zearalenon (ZEN) on injury of pig intestinal epithelial cells (IPEC-J2 cells). The experiment set the control group (C group), ZEN group (30 μg/mL), ZEN+0.5 Se group (30 μg/mL ZEN+0.5 μmol/L oligomeric chitosan selenium, calculated as Se), ZEN+1.5 Se group (30 μg/mL ZEN+1.5 μmol/L oligomeric chitosan selenium, calculated as Se), ZEN+3 Se group (30 μg/mL ZEN+3 μmol/L oligomeric chitosan selenium, calculated as Se). The survival rate, lactate dehydrogenase (LDH) activity, reactive oxygen species (ROS) content, changes in mitochondrial membrane potential, protein expression related to oxidative stress and endoplasmic reticulum stress were measured. The results showed that compared with control group, adding ZEN significantly increased LDH activity (P<0.05) and extremely significantly increased ROS content (P<0.01), and extremely significantly decreased mitochondrial membrane potential (P<0.01) and significantly decreased survival rate (P<0.05); the addition of oligomeric chitosan selenium were significantly reduced LDH activity (P<0.05), extremely significantly reduced ROS content (P<0.01), and significantly increased mitochondrial membrane potential (P<0.05) and extremely significantly increased survival rate (P<0.01). Compared with the control group, adding ZEN significantly reduced transcription factor E2 related factors (Nrf2) protein expression (P<0.05), Nrf2 protein expression was significantly alleviated by the addition of oligomeric chitosan selenium (P<0.01); compared with the control group, haem oxygenase-1 (HO-1) protein expression in the ZEN group and ZEN+0.5 Se group were significantly lower (P<0.05), there was no significant difference among ZEN+0.5 Se, ZEN+1.5 Se, ZEN+3.0 Se and ZEN groups (P>0.05), however, there was no significant difference among ZEN+1.5 Se, ZEN+3.0 Se and control group (P>0.05). Compared with the control group, adding ZEN significantly increased glucose-regulatory protein (GRP78) (P<0.05), endoplasmic reticulum regulated kinase (P-PERK/PERK) (P<0.05), transcriptional activator 4 (ATF4) (P<0.05), C/EBP homologous protein (CHOP) (P<0.01) protein expression; the supplementation of oligomeric chitosan selenium significantly decreased GRP78 (P<0.05), P-PERK/PERK (P<0.01), ATF4 (P<0.01), CHOP (P<0.05) protein expression. In conclusion, oligomeric chitosan selenium can alleviate oxidative damage and endoplasmic reticulum stress of IPEC-J2 cells induced by ZEN.

Cite this article

FU Chunni , LI Yuanhui , LI Pengcheng , LI Liuan , QIN Shunyi . Effects of Oligomeric Chitosan Selenium on Oxidative Damage and Endoplasmic Reticulum Stress in Zearalenon-Induced Pig Intestinal Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 5226 -5235 . DOI: 10.3969/j.issn.1006-267x.2021.09.042

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