MOLECULAR AND CELLULAR NUTRITION

Effects of Ethanol Extract of Artemisia annua on Milk Protein Synthesis in Lipopolysaccharide-Induced Injury Mammary Cells of Dairy Cows

  • HU Yao ,
  • SONG Jie ,
  • WANG Lifang ,
  • AO Changjin
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  • 1. Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Laboratory of Quality & Safety Risk Assessment for Agricultural Products(Hohhot), Ministry of Agriculture and Rural Affairs, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China

Received date: 2021-02-27

  Online published: 2021-09-18

Abstract

This experiment used a bovine mammary epithelial cells (BMECs) in vitro culture technology, to investigate the effects of ethanol extract of Artemisia annulus (AAE) on viability and expression of milk protein biosynthesis related genes of BMECs by lipopolysaccharide (LPS)-induced injury. The experiment was divided into 5 groups, control group:BMECs were cultured in basic medium for 15 h; model group:BMECs were cultured in basic medium for 3 h+10 μg/mL LPS treated 12 h; AAE+LPS groups:BMECs were cultured in basic medium contained 3, 6 and 12 AAE for 3 h+10 μg/mL LPS treated 12 h. The results showed as follows:1) compared with the control group, the BMECs viability of the model group was significantly decreased (P<0.05). Compared with the model group, the BMECs viability of 6 and 12 μg/mL AAE groups was significantly increased (P<0.05). 2) Compared with the model group, the total protein content of 12 μg/mL AAE group was significantly increased (P<0.05), the β-casein content of 6 and 12 μg/mL AAE groups was significantly increased (P<0.05), and the αS1-casein content of 3, 6 and 12 μg/mL AAE groups was significantly increased (P<0.05). 3) Compared with the model group, the α-casein (CSN1S1) gene expression level of 12 μg/mL AAE group was significantly increased (P<0.05), the gene expression levels of β-casein (CSN2), mammalian target of rapamycin (mTOR), signal transducers and activators of transduction 5 (STAT5), recombinant eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1), ribosomal S6 protein kinase 1 (S6K1) and Janus kinase 2 (JAK2) of 3, 6 and 12 μg/mL AAE groups were significantly increased (P<0.05), and the eukaryotic initiation factor 4E (EIF4E) gene expression level of 6 and 12 μg/mL AAE group was significantly increased (P<0.05). In conclusion, 12 μg/mL AAE has better pre-protection effects on viability and milk protein biosynthesis of BMECs by LPS-induced injury.

Cite this article

HU Yao , SONG Jie , WANG Lifang , AO Changjin . Effects of Ethanol Extract of Artemisia annua on Milk Protein Synthesis in Lipopolysaccharide-Induced Injury Mammary Cells of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 5236 -5245 . DOI: 10.3969/j.issn.1006-267x.2021.09.043

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