Chitosan Alleviates Lipopolysaccharide-Induced Oxidative Stress Damage in Peripheral Blood Mononuclear Cells of Dairy Cows by Inhibiting Interleukin-1&beta; Biological Activity

QI Jingyu; ZHENG Yaguang; HAO Ying; ZHAO Yanli; GUO Xiaoyu; GUO Yongmei; SHI Binlin; YAN Sumei

doi:10.3969/j.issn.1006-267x.2021.09.044

2021 , Vol. 33 >Issue 9: 5246 - 5255

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.09.044

MOLECULAR AND CELLULAR NUTRITION

Chitosan Alleviates Lipopolysaccharide-Induced Oxidative Stress Damage in Peripheral Blood Mononuclear Cells of Dairy Cows by Inhibiting Interleukin-1β Biological Activity

QI Jingyu ,
ZHENG Yaguang ,
HAO Ying ,
ZHAO Yanli ,
GUO Xiaoyu ,
GUO Yongmei ,
SHI Binlin ,
YAN Sumei

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College of Animal Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2021-02-27

Online published: 2021-09-18

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Abstract

In this experiment, lipopolysaccharide (LPS) was used as the stimulation source, using interleukin-1 receptor antagonist (IL-1ra) to block the biological activity of interleukin-1β (IL-1β), to investigate the mitigating effect of chitosan (COS) on LPS-induced oxidative stress damage in peripheral blood mononuclear cells (PBMC) of dairy cows. With a one-way completely randomized trial design, PBMC were randomly divided into 8 groups, namely, control group (cultured in a basic medium without COS, LPS and IL-1Ra for 72 h), COS group (cultured in a medium with COS only for 72 h), LPS group (cultured in the basic medium for 48 h, and then added LPS for another 24 h), IL-1ra group (cultured in the basic medium for 42 h, and then added IL-1ra for another 30 h), COS+IL-1ra group (cultured in the medium with COS for 42 h, and then added IL-1ra for another 30 h), COS+LPS group (cultured in the medium with COS for 48 h, and then added LPS for another 24 h), IL-1ra+LPS group (cultured in the basic medium for 42 h, and then added IL-1ra for another 6 h, then added LPS for another 24 h), and COS+IL-1ra+LPS group (cultured in the medium with COS for 42 h, and then added IL-1ra for another 6 h, then added LPS for another 24 h), with 6 replicates in each group. The results showed that the activities of antioxidant enzymes glutathione peroxidase (GPx), total superoxide dismutase (T-SOD), catalase (CAT) and thioredoxin reductase (TrxR), the total antioxidant capacity (T-AOC) and the mRNA relative expression levels of GPx1 and TrxR were significantly decreased in the LPS group compared with the control group (P ≤ 0.05), while the activity of inducible nitric oxide synthase (iNOS), the contents of inflammatory factors IL-1β, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) and their mRNA relative expression levels, the mRNA relative expression level of nuclear factor-κB (NF-κB) p65, the contents of nitric oxide (NO) and malondialdehyde (MDA), and the reactive oxygen species (ROS) activity were significantly increased (P ≤ 0.05), it indicated that LPS-induced oxidative stress damage in PBMC of dairy cows was caused by its activation of NF-κB signaling pathway, leading to the release of inflammatory factor IL-1β and an increase in iNOS activity and NO content. Compared with the LPS group, the above antioxidant enzyme activities and the mRNA relative expression levels of GPx1 and TrxR were significantly increased in the LPS+IL-1ra and LPS+COS groups (P ≤ 0.05), whereas the contents of the above inflammatory factors and their mRNA relative expression levels, as well as the mRNA relative expression level of NF-κB p65 were significantly decreased (P ≤ 0.05), it indicated that IL-1ra and COS had similar preventive effects on LPS-induced oxidative stress damage in PBMC of dairy cows. In summary, it is clear that COS mitigates LPS-induced oxidative stress damage in PBMC of dairy cows by inhibiting the biological activity of IL-1β, which may be related to COS inhibiting the activity of NF-κB signaling pathway and thus reducing the release of IL-1β and the production of NO.

Key words： chitosan; PBMC; oxidative stress; interleukin-1 receptor antagonist

Cite this article

QI Jingyu , ZHENG Yaguang , HAO Ying , ZHAO Yanli , GUO Xiaoyu , GUO Yongmei , SHI Binlin , YAN Sumei . Chitosan Alleviates Lipopolysaccharide-Induced Oxidative Stress Damage in Peripheral Blood Mononuclear Cells of Dairy Cows by Inhibiting Interleukin-1β Biological Activity[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 5246 -5255 . DOI: 10.3969/j.issn.1006-267x.2021.09.044

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