EXPERIMENTAL METHOD AND ANIMAL

Effects of Different Types of Dietary Fibers on Growth Performance, Biochemical Indexes in Serum and Indexes Related to Immunity of Mice with Colitis Induced by Dextran Sulfate Sodium

  • LAN Cong ,
  • HE Jun ,
  • LI Hua ,
  • TANG Jiayong ,
  • CHEN Daiwen ,
  • YU Bing ,
  • HE Jun ,
  • HUANG Zhiqing ,
  • ZHENG Ping ,
  • MAO Xiangbing ,
  • YU Jie ,
  • LUO Junqiu ,
  • YAN Hui ,
  • LUO Yuheng
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  • Sichuan Provincial Engineering Laboratory of Livestock and Poultry Nutrition & Feed, Animal Disease Resistance Nutrition Key Laboratory of Sichuan Province, Key Laboratory of Animal Disease Resistance Nutrition and Feed Science Ministry of Agriculture of China, Key Laboratory for Animal Disease Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute of Sichuan Agricultural University, Chengdu 611130, China

Received date: 2021-01-26

  Online published: 2021-09-18

Abstract

This study was conducted to compare the effects of inulin (typical soluble dietary fiber) and microcrystalline cellulose (MCC, typical insoluble dietary fiber) on the growth performance, biochemical indexes in serum and indexes related to immunity of mice with colitis induced by dextran sulfate sodium (DSS). Twenty-four healthy male C57BL/6 mice aged 8 weeks were selected and 3% DSS was added to drinking water for 7 days to induce colitis. Then, mice with DSS-induced colitis were randomly allocated to three groups. Mice in C group (control group) were fed a fiber-free diet, while mice in I and M groups were fed diets containing 5% inulin or MCC for 14 days, respectively. At the end of the animal trial, all mice were sacrificed, and venous blood and colon samples were sampled. The results showed as follows:dietary supplementation of 5% inulin or MCC had no significant differences on the growth performance and the indexes of liver, spleen and thymus during recovery period (P>0.05). Compared with C group, the content of C-reactive protein in serum of mice in I and M groups was significantly decreased (P<0.05), while the content of non-esterified fatty acid in serum of mice in I group was significantly increased (P<0.05). Compared with M group, the content of non-esterified fatty acid in serum of mice in I group was significantly increased (P<0.05), and the content of immunoglobulin M in serum of mice tended to decrease (P=0.061). Meanwhile, compared with C group, the percentages of CD3+ (P=0.053) and CD4+ (P=0.091) T lymphocytes in spleen of mice in I and M groups had a tendency to increase. The mRNA relative expression levels of Toll like receptor 4 (TLR4) and receptor-interacting serine/threonine protein kinase 2 (RIPK2) in colon of mice in M group were significantly decreased compared with C group (P<0.05), and the mRNA relative expression levels of TLR4, tumor necrosis factor receptor associated factor 6 (TRAF6) and RIPK2 in colon of these mice showed significantly decreased when compared with I group (P<0.05). Therefore, both the dietary supplementation of 5% inulin and MCC can improve the colonic immune function of mice with DSS-induced colitis, and the MCC can regulate colonic immunity by downregulating the expression of genes associated with TLR4 or nucleotide-binding oligomerization domains-like receptor (NLR) pathways. These results indicate that the underlying mechanism of different types of dietary fibers on the intestinal health in mice with colitis may be different.

Cite this article

LAN Cong , HE Jun , LI Hua , TANG Jiayong , CHEN Daiwen , YU Bing , HE Jun , HUANG Zhiqing , ZHENG Ping , MAO Xiangbing , YU Jie , LUO Junqiu , YAN Hui , LUO Yuheng . Effects of Different Types of Dietary Fibers on Growth Performance, Biochemical Indexes in Serum and Indexes Related to Immunity of Mice with Colitis Induced by Dextran Sulfate Sodium[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 5277 -5289 . DOI: 10.3969/j.issn.1006-267x.2021.09.047

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