Effects of Lactobacillus rhamnosus on Growth Performance, Immune Performance and Intestinal Microflora of Early Weaned Chenghua Black Piglets

ZHU Jiawen; XU Qilin; SU Zhipeng; LIU Hanyang; CHEN Yaying; YE Siqi; YANG Xue; LEI Chunlong; ZHENG Jiangrong; XU Zhenying

doi:10.3969/j.issn.1006-267x.2021.09.051

2021 , Vol. 33 >Issue 9: 5324 - 5331

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.09.051

SHORT COMMUNICATION

Effects of Lactobacillus rhamnosus on Growth Performance, Immune Performance and Intestinal Microflora of Early Weaned Chenghua Black Piglets

ZHU Jiawen ,
XU Qilin ,
SU Zhipeng ,
LIU Hanyang ,
CHEN Yaying ,
YE Siqi ,
YANG Xue ,
LEI Chunlong ,
ZHENG Jiangrong ,
XU Zhenying

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1. Chengdu Academy of Agricultural and Forestry Sciences, Chengdu 611130, China;
2. Agricultural and Rural Bureau of Shizhong District, Leshan City, Leshan 614000, China;
3. Sichuan Dekang Agriculture and Animal Husbandry Food Group Co., Ltd., Chengdu 610200, China;
4. College of Veterinary Medicine, Southwest University, Chongqing 400700, China

Received date: 2021-02-10

Online published: 2021-09-18

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Abstract

This experiment was conducted to investigate the effects of Lactobacillus rhamnosus on the growth performance, immune performance and intestinal microflora of early weaned Chenghua black piglets. Thirty-two Chenghua black piglets weaned at 28 days of age with body weight of (7.54±0.19) kg were randomly divided into 2 groups with 4 replicates per group and 4 piglets per replicate. Piglets in the control group were fed a basal diet, and those in the Lactobacillus rhamnosus group were fed the basal diet supplemented with 5.56 mg/kg Lactobacillus rhamnosus (the viable bacteria count reached 2.78×10⁹ CFU/kg diet). The experiment lasted for 28 days after 7-day adaption. The results showed as follows:1) compared with the control group, the average daily gain and gain to feed ratio of weaned piglets in the Lactobacillus rhamnosus group were significantly increased (P<0.05). 2) Compared with the control group, the contents of total protein, tumor necrosis factor-α, interleukin-2, complement 3 and immunoglobulin G, the activity of catalase and superoxide dismutase and total antioxidant capacity in serum of weaned piglets in the Lactobacillus rhamnosus group were significantly increased (P<0.05). 3) The high-throughput sequencing of 16S rDNA gene showed that Firmicutes, Bacteroidetes and Proteobacteria were the dominant bacteria phyla in the cecal contents of weaned piglets. At the genus level, compared with the control group, the relative abundances of Lactobacillus and Alloprevotella in the cecal contents of the Lactobacillus rhamnosus group were increased by 21.00 times and 3.79 times, respectively; and the relative abundances of Prevotella_9 and Escherichia-Shigella in the cecal contents were decreased by 54.54% and 70.62%, respectively. In conclusion, dietary Lactobacillus rhamnosus can increase the average daily gain and gain to feed ratio of early weaned Chenghua black piglets, improve the serum antioxidant capacity and immune performance, increase the relative abundances of Lactobacillus and Alloprevotella in cecal contents, reduce the relative abundance of Escherichia-Shigella in cecal contents, improve the intestinal microflora structure, promote the intestinal health, and thus improve their growth performance and immune performance.

Key words： growth performance; immune performance; intestinal microflora; Lactobacillus rhamnosus; early weaned piglets

Cite this article

ZHU Jiawen , XU Qilin , SU Zhipeng , LIU Hanyang , CHEN Yaying , YE Siqi , YANG Xue , LEI Chunlong , ZHENG Jiangrong , XU Zhenying . Effects of Lactobacillus rhamnosus on Growth Performance, Immune Performance and Intestinal Microflora of Early Weaned Chenghua Black Piglets[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 5324 -5331 . DOI: 10.3969/j.issn.1006-267x.2021.09.051

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