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Effects of Protease Supplementation in Low Protein Diet on Growth Performance, Liver Growth Gene and Target of Rapamycin Signaling Pathway Gene Expression of Broilers

  • ZHANG Jingjing ,
  • ZHENG Xu ,
  • LI Yue ,
  • ZHU Suiliang ,
  • WANG Shiqiong ,
  • ZHANG Wei ,
  • QIAO Yinging ,
  • LIU Changzhong ,
  • WANG Zhixiang
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  • 1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China;
    2. Henan Institute of Science and Technology, Xinxiang 453003, China

Received date: 2021-02-11

  Online published: 2021-09-18

Abstract

This experiment was conducted to investigate the effects of protease supplementation in low protein diet on the growth performance, serum biochemical parameters, liver key growth gene and target of rapamycin (TOR) signaling pathway gene expression of broilers. A total of 324 one-day-old Arbor Acres (AA) white-feathered broilers were randomly divided into 3 groups with 6 replicates per group and 18 broilers per replicate (half male and half female). Birds in the control group were fed a basal diet (the crude protein level was 23% in the early stage and 20% in the late stage); the diet in the low protein group (the crude protein level was 21% in the early stage and 18% in the late stage) was supplemented with L-Lys·HCl, DL-Met and L-Thr to meet the feeding standard for AA broilers; and the diet in the low protein plus enzyme group was supplemented with 500 mg/kg protease (enzyme activity was 30 000 U/g) on the basis of the low protein group. The experiment lasted for 42 days. The results showed as follows:1) the body weight of broilers at 21 and 42 days of age and the average daily gain (ADG) from 1 to 21 days of age and 1 to 42 days of age in the low protein plus enzyme group were significantly higher than those in the low protein group (P<0.05), the ADG from 1 to 21 days of age was significantly higher than that in the control group (P<0.05), and the ratio of feed to gain was significantly lower than that in the control group and the low protein group (P<0.05). 2) The serum uric acid content of broilers in the low protein group and the low protein plus enzyme group was significantly lower than that in the control group (P<0.05), the serum urea nitrogen content in the low protein plus enzyme group was significantly lower than that in the control group (P<0.05), and the serum total cholesterol content in the low protein group was significantly higher than that in the control group and the low protein plus enzyme group (P<0.05). 3) The expression level of insulin-like growth factor binding protein 1 (IGFBP1) gene in liver of broilers in the low protein plus enzyme group was significantly lower than that in the control group and the low protein group (P<0.05), while the expression levels of insulin-like growth factor-Ⅰ (IGF-Ⅰ), growth hormone (GH) and growth hormone receptor (GHR) genes in liver in the low protein plus enzyme group were significantly higher than those in the low protein group (P<0.05). 4) The rates of chest muscle and leg muscle and the expression levels of TOR, ribosomal protein S6 kinase β1 (S6K1) and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) genes in chest muscle and leg muscle of broilers in the low protein plus enzyme group were significantly higher than those in the low protein group (P<0.05). In summary, the low protein diet supplemented with protease can increase the expression levels of liver key growth genes and genes related to TOR signaling pathway, promote the protein synthesis and metabolism, increase the protein deposition in skeletal muscle, and improve the growth performance of broilers.

Cite this article

ZHANG Jingjing , ZHENG Xu , LI Yue , ZHU Suiliang , WANG Shiqiong , ZHANG Wei , QIAO Yinging , LIU Changzhong , WANG Zhixiang . Effects of Protease Supplementation in Low Protein Diet on Growth Performance, Liver Growth Gene and Target of Rapamycin Signaling Pathway Gene Expression of Broilers[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 5332 -5344 . DOI: 10.3969/j.issn.1006-267x.2021.09.052

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