Analysis of Microbial Composition and Diversity in Cecum of Laying Ducks with Different Residual Feed Intake

YANG Su; BAI Tian; CAO Yongqing; HUANG Xuetao; CHEN Bindan; WANG Wuqiang; SHEN Junda; TIAN Yong; LI Guoqin; TAO Zhengrong; CHEN Li; ZHAO Ayong; LU Lizhi; ZENG Tao

doi:10.3969/j.issn.1006-267x.2021.09.053

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 9: 5345 - 5355

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.09.053

SHORT COMMUNICATION

Analysis of Microbial Composition and Diversity in Cecum of Laying Ducks with Different Residual Feed Intake

YANG Su ,
BAI Tian ,
CAO Yongqing ,
HUANG Xuetao ,
CHEN Bindan ,
WANG Wuqiang ,
SHEN Junda ,
TIAN Yong ,
LI Guoqin ,
TAO Zhengrong ,
CHEN Li ,
ZHAO Ayong ,
LU Lizhi ,
ZENG Tao

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1. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;
2. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. College of Animal Science and Technology, Zhejiang A&F University, Hangzhou 311300, China;
4. College of Animal Science and Technology, Gansu Agriculture University, Lanzhou 730000, China;
5. Zhuji Guowei Poultry Industry Development Co., Ltd., Shaoxing 311800, China;
6. Jinyun County Ministry of Agriculture and Rural Affairs, Lishui 321400, China

Received date: 2021-02-27

Online published: 2021-09-18

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Abstract

The objective of this study was to analyze the composition and diversity differences of microorganism in cecum of laying ducks with different residual feed intake (RFI). Three hundred 400-day-old Shaoxing ducks were chosen and reared in single cages, the individual initial body weight, final body weight, feed intake and average egg weight were recorded during the experiment, and the multiple linear regression equations were used to calculate individual RFI. Fifteen ducks with the highest RFI and the lowest RFI were selected, which were high RFI (HRFI) group and low RFI (LRFI) group, respectively. After collecting the cecal contents, the microbial genomic DNA was extracted for high-throughput sequencing by using 16S rDNA technique, and to analyze the bacterial community structure. The results showed as follows:1) the RFI, feed intake and feed to egg ratio of HRFI group were significantly higher than those of LRFI group (P<0.01). There were no significant differences in weight gain, average egg weight, laying rate and body weight between 2 groups (P>0.05). 2) The Chao1 index and Shannon index of LRFI group were significantly higher than those of HRFI group (P<0.05). 3) At the phylum level, Firmicutes was the dominant bacteria in cecum of LRFI group, while Proteobacteria was the dominant bacteria in HRFI group, and Bacteroidetes had little difference between 2 groups. 4) At the genus level, there were 18 different bacterial communities between the HRFI and LRFI groups and the relative abundance of the top 10 microorganism genus composition in the 2 groups were the same. The relative abundance of Christensenellaceae_R-7_group, Oribacterium and Oribacterium in cecum of LRFI group were significantly higher than those of HRFI group (P<0.01), and the Mycoplasma relative abundance of HRFI group was significantly higher than that of LRFI group (P<0.01). In conclusion, the cecal microbial diversity of laying ducks of LRFI group is higher, and there are differences in microbial community structure between the 2 groups. At the phylum level, Firmicutes is the dominant bacterial community in cecum of LRFI group, while Proteobacteria is the dominant bacterial community in HRFI group; at the genus level, the relative abundance of 17 bacteria such as Christensenellaceae_R-7_group, Oribacterium and Oribacterium of LRFI group is higher than that of HRFI group, while only Mycoplasma relative abundance of HRFI group is higher than that of LRFI group.

Key words： RFI; laying ducks; cecal microorganism; 16S rDNA

Cite this article

YANG Su , BAI Tian , CAO Yongqing , HUANG Xuetao , CHEN Bindan , WANG Wuqiang , SHEN Junda , TIAN Yong , LI Guoqin , TAO Zhengrong , CHEN Li , ZHAO Ayong , LU Lizhi , ZENG Tao . Analysis of Microbial Composition and Diversity in Cecum of Laying Ducks with Different Residual Feed Intake[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 5345 -5355 . DOI: 10.3969/j.issn.1006-267x.2021.09.053

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