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Effects of Dietary Sodium Butyrate on Non-Specific Immune, Antioxidant Indices and Intestinal Mucosal Morphology of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

  • WANG Hairui ,
  • HU Junru ,
  • ZHAO Hongxia ,
  • CAO Junming ,
  • CHEN Bing ,
  • CHEN Xiaoying
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  • 1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
    2. Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    3. Guangzhou Fishtech Biotechnology Co., Ltd., Guangzhou 510640, China

Received date: 2021-03-15

  Online published: 2021-09-18

Abstract

This experiment was conducted to investigate the effects of dietary sodium butyrate on non-specific immune, antioxidant indices and intestinal mucosal morphology of juvenile yellow catfish (Pelteobagrus fulvidraco). Five isonitrogenous and isolipidic experimental diets were formulated to contain sodium butyrate (SB) at 0, 250, 500, 1 000 and 2 000 mg/kg, respectively. A total of 600 juvenile yellow catfish with the average body weight of (1.26±0.01) g were randomly divided into 5 groups with 3 replicates per group and 40 fish per replicate, and fish in the 5 groups fed their respective diet from the five isonitrogenous and isolipids diets for 56 days. The results showed as follows:the activities of serum lysozyme (LZM)and nitric oxide synthase (NOS) in 1 000 mg/kg BS group and the activity of serum alkaline phosphatase (AKP) in 250 mg/kg SB group were the highest, and significantly higher than those in no-added group (P<0.05). The activities of liver LZM and AKP in 1 000 mg/kg BS group were the highest, and significantly higher than those in no-added group (P<0.05). The activities of intestinal AKP and NOS in 500 mg/kg BS group were the highest, and significantly higher than those in no-added and 2 000 mg/kg SB group (P<0.05). Serum catalase (CAT) and glutathione peroxidase (GSH-Px) activities in 2 000 SB mg/kg group were significantly higher than those in no-added group (P<0.05). The activities of serum superoxide dismutase (SOD) and liver GSH-Px in 1 000 mg/kg SB group were significantly higher than those in no-added group (P<0.05). There were significantly higher activities of intestinal SOD, CAT and GSH-Px in 500 mg/kg SB group than those in no-added group (P<0.05), and significantly lower content of intestinal malondialdehyde (MDA) in 250, 500, 1 000 and 2 000 mg/kg groups than those in no-added group (P<0.05). Villus length, villus width and muscular thickness in proximal intestine and villus length, muscular thickness in distal intestine were significantly higher in 500 mg/kg SB group compared with no-added group (P<0.05). With the serum NOS, SOD activities and villus length of proximal intestine as evaluation indices, the optimum dietary SB supplemental levels are 1 275, 1 218 and 1 489 mg/kg through quadratic regression analysis, respectively.

Cite this article

WANG Hairui , HU Junru , ZHAO Hongxia , CAO Junming , CHEN Bing , CHEN Xiaoying . Effects of Dietary Sodium Butyrate on Non-Specific Immune, Antioxidant Indices and Intestinal Mucosal Morphology of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)[J]. Chinese Journal of Animal Nutrition, 2021 , 33(9) : 5379 -5390 . DOI: 10.3969/j.issn.1006-267x.2021.09.056

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