Effects of Dietary Tryptophan Levels on Growth Performance, Serum Biochemical Indices, Organ Indexes and Intestinal Morphology of Broilers

FU Chunyan; DONG Yilei; YAN Peipei; WANG Wenbin; YAN Lei; HUANG He; ZHU Yingbo; WEI Xiangfa; LIU Ruiting; SHI Tianhong; LIU Xuelan

doi:10.3969/j.issn.1006-267x.2021.10.015

2021 , Vol. 33 >Issue 10: 5545 - 5556

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.015

POULTRY NUTRITION AND FEED

Effects of Dietary Tryptophan Levels on Growth Performance, Serum Biochemical Indices, Organ Indexes and Intestinal Morphology of Broilers

FU Chunyan ,
DONG Yilei ,
YAN Peipei ,
WANG Wenbin ,
YAN Lei ,
HUANG He ,
ZHU Yingbo ,
WEI Xiangfa ,
LIU Ruiting ,
SHI Tianhong ,
LIU Xuelan

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1. Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China;
2. Shandong New Hope Liuhe Group Co., Ltd., Qingdao 266100, China;
3. Dongying Blue Sea Ecological Aquaculture Company, Dongying 257100, China

Received date: 2021-03-16

Online published: 2021-10-16

Supported by

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Abstract

This experiment was conducted to investigate the effects of tryptophan, as functional amino acids, on growth performance, serum biochemical indices, organ indexes, and intestinal morphology of broilers. A single factor and six addition levels of trial was designed. A total of 360 one-day-old male Arbor Acres broilers were randomly divided into 6 groups with 6 replicates per group and 20 birds per replicate. Broilers in the control group were fed a basal diet (the tryptophan level was 0.23% at 1 to 21 days of age and 0.20% at 22 to 42 days of age), and others in the 5 experimental groups were fed the basal diet supplemented with 0.03%, 0.06%, 0.09%, 0.12% and 0.15% tryptophan, respectively. The chickens were free to feed and drink. And the experiment lasted for 42 days. The results showed as follows:1) compared with the control group, 0.03% and 0.12% tryptophan supplementation significantly reduced the body weight of broilers at 21 days of age (P<0.05), while 0.03%, 0.09% and 0.12% tryptophan supplementation significantly decreased the average daily feed intake (ADFI), the average daily gain (ADG) and the feed to gain ratio (F/G) of broiler from 1 to 21 days of age (P<0.05). The supplementation of tryptophan in basal diets has no significant influence on the body weight of broilers at 42 days of age, and the ADFI, ADG, and feed/gain (F/G) of broilers at 22 to 42 days of age, or 1 to 42 days of age, as well as the F/G of broilers at 1 to 21 days of age (P>0.05). 2) Compared with control group, 0.12% tryptophan supplementation significantly increased the thymus index (P<0.05), but significantly decreased the liver index of broilers at 21 days of age (P<0.05). 0.15% tryptophan supplementation significantly decreased the thymus index and liver index of broilers at 21 days of age (P<0.05). The thymus index significantly increased quadratically with the supplementation of tryptophan (P<0.05), while the liver index was significantly decreased linearly and quadratically (P<0.05). The supplementation of tryptophan in basal diet had no influence on the organ indexes of broilers at 42 days of age (P>0.05). 3) Compared with the control group, the basal diet supplemented with 0.06% tryptophan significantly increased the activity of superoxide dismutase (SOD) in serum of broilers at 21 and 42 days of age (P<0.05). Furthermore, the serum SOD activity significantly increased linearly and quadratically (P<0.05) with the supplementation of tryptophan. 4) 0.09% tryptophan significantly increased the serum immunoglobulin A content in broilers at 21 days of age (P<0.05). 5) Compared with the control group, the basal diet supplemented with 0.12% and 0.15% tryptophan significantly decreased the duodenum villus height (VH) of broilers at 21 days of age (P<0.05), and significantly increased the jejunum ileum VH and crypt depth (CD, P<0.05). For the broilers at 42 days of age, tryptophan supplementation in the basal diet had no significant influence on the development of duodenum and jejunum (P>0.05), but the jejunum VH and villus height/crypt depth (V/C) decreased linearly (P<0.05), as well as that of ileum. In conclusion, tryptophan supplementation in basal diet has no significant influence on the growth performance, organ indexes and the development of jejunum and ileum of broilers in later growth stage, but can increase the serum antioxidant and immune ability of broilers.

Key words： tryptophan; broilers; growth performance; immune and antioxidant functions; organ indexes; intestinal morphology

Cite this article

FU Chunyan , DONG Yilei , YAN Peipei , WANG Wenbin , YAN Lei , HUANG He , ZHU Yingbo , WEI Xiangfa , LIU Ruiting , SHI Tianhong , LIU Xuelan . Effects of Dietary Tryptophan Levels on Growth Performance, Serum Biochemical Indices, Organ Indexes and Intestinal Morphology of Broilers[J]. Chinese Journal of Animal Nutrition, 2021 , 33(10) : 5545 -5556 . DOI: 10.3969/j.issn.1006-267x.2021.10.015

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