Effects of Compound Preparation of Enzyme and Probiotics on Growth Performance, Immune Function and Antioxidant Function of Broilers

CHEN Dandie; PENG Xiang; ZHANG Guangmin; CHEN Kaixuan; SU Yanfang; SA Renna; HUANG Yanling

doi:10.3969/j.issn.1006-267x.2021.10.016

2021 , Vol. 33 >Issue 10: 5557 - 5568

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.016

POULTRY NUTRITION AND FEED

Effects of Compound Preparation of Enzyme and Probiotics on Growth Performance, Immune Function and Antioxidant Function of Broilers

CHEN Dandie ,
PENG Xiang ,
ZHANG Guangmin ,
CHEN Kaixuan ,
SU Yanfang ,
SA Renna ,
HUANG Yanling

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1. College of Animal and Veterinary Science, Southwest Minzu University, Chengdu 610041, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Tianjin Bofeider Technology Limited Company, Tianjin 300000, China

Received date: 2021-03-11

Online published: 2021-10-16

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Abstract

This experiment was conducted to compare and analyze the effects of different combinations of enzyme and probiotics compound preparation on growth performance, immune function and antioxidant function of broilers, so as to provide support for the development of reasonable alternatives to antibiotic growth promoters. A total of 1 120 healthy Cobb 500 broiler chickens of one-day-old were randomly assigned into 4 groups with 10 replicates per group and 28 chickens (half male and half female) per replicate. The four groups were as follows:1) the blank control group, and the broilers were fed a basal diet; 2) the antibiotic group, and the broilers were fed the basal diet supplemented with 45 g/t aureomycin (15%) and 10 g/t kitasamycin (50%); 3) the compound preparation of enzyme and probiotics group Ⅰ, and the broilers were fed the basal diet supplemented with 150 g/t non-starch polysaccharide enzyme, 300 g/t glucose oxidase (GOD) and 50 g/t lactic acid bacteria; 4) the compound preparation of enzyme and probiotics group Ⅱ, and the broilers were fed the basal diet supplemented with 500 g/t quorum quenching enzyme (QQE), 300 g/t GOD and 50 g/t lactic acid bacteria. The basal diet was corn-soybean meal type. The experiment lasted for 42 days, during which the chickens were free to eat and drink. The results showed as follows:1) compared with the blank control group, at the early growth stage (1 to 21 days of age), the final body weight and average daily gain of broilers in the antibiotic group and the compound preparation of enzyme and probiotics group Ⅱ were significantly increased (P<0.05), and the ratio of feed to gain was significantly decreased (P<0.05). There was no significant difference in growth performance between the antibiotic group and the compound preparation of enzyme and probiotics group Ⅱ (P<0.05). There were no significant differences in the growth performance indices of broilers in the late growth period (22 to 42 days of age) and the whole growth period (1 to 42 days of age) among all groups (P>0.05). 2) Compared with the blank control group, the serum interleukin-6 (IL-6) content of broilers in the antibiotic group and the compound preparation of enzyme and probiotics group Ⅱ was significantly increased (P<0.05), and the serum interleukin-10 content had a tendency to increase (0.05 ≤ P<0.10). 3) Compared with the blank control group, the activities of glutathione peroxidase (GSH-Px) and catalase (CAT) in serum of broilers in the compound preparation of enzyme and probiotics group Ⅱ were significantly increased (P<0.05), the serum CAT activity of broilers in the compound preparation of enzyme and probiotics group Ⅰ was significantly increased (P<0.05), and the activity of superoxide dismutase (SOD) in liver of broilers in the compound preparation of enzyme and probiotics groups Ⅰ and Ⅱ was significantly increased (P<0.05). 4) Compared with the blank control group, the uric acid content in feces of broilers in the antibiotic group, the compound preparation of enzyme and probiotics groups Ⅰ and Ⅱ was significantly decreased (P<0.05). In conclusion, dietary enzyme and probiotics compound preparation composed of QQE, GOD and lactic acid bacteria can improve the growth performance of broilers during early growth period (1 to 21 days of age), significantly increase the serum IL-6 content, the serum CAT and GSH-Px activities and the liver SOD activity, and significantly reduce the fecal uric acid content, thus can effectively replace antibiotics.

Key words： compound preparation of enzyme and probiotics; antibiotics; broilers; growth performance; immune function; antioxidant function

Cite this article

CHEN Dandie , PENG Xiang , ZHANG Guangmin , CHEN Kaixuan , SU Yanfang , SA Renna , HUANG Yanling . Effects of Compound Preparation of Enzyme and Probiotics on Growth Performance, Immune Function and Antioxidant Function of Broilers[J]. Chinese Journal of Animal Nutrition, 2021 , 33(10) : 5557 -5568 . DOI: 10.3969/j.issn.1006-267x.2021.10.016

References

[1] ARFC R F.Probiotics in man and animals[J].Journal of Applied Bacteriology, 1989, 66(5):365-378.

[2] SHOKRYAZDAN P, FASELEH JAHROMI M, LIANG J B, et al.Effects of a Lactobacillus salivarius mixture on performance, intestinal health and serum lipids of broiler chickens[J].PLoS One, 2017, 12(5):e0175959.
[3] HOUSHMAND M, AZHAR K, ZULKIFLI I, et al.Effects of non-antibiotic feed additives on performance, tibial dyschondroplasia incidence and tibia characteristics of broilers fed low-calcium diets[J].Journal of Animal Physiology and Animal Nutrition, 2011, 95(3):351-358.

[4] KHAKSEFIDI A, GHOORCHI T.Effect of probiotic on performance and immunocompetence in broiler chicks[J].The Journal of Poultry Science, 2006, 43(3):296-300.

[5] MENG X, SLOMINSKI B A, NYACHOTI C M, et al.Degradation of cell wall polysaccharides by combinations of carbohydrase enzymes and their effect on nutrient utilization and broiler chicken performance[J].Poultry Science, 2005, 84(1):37-47.

[6] SCHEIDELER S E, BECK M M, ABUDABOS A, et al.Multiple-enzyme (Avizyme) supplementation of corn-soy-based layer diets[J].Journal of Applied Poultry Research, 2005, 14(1):77-86.

[7] BRENES A, VIVEROS A, ARIJA I, et al.The effect of citric acid and microbial phytase on mineral utilization in broiler chicks[J].Animal Feed Science and Technology, 2003, 110(1/4):201-219.
[8] TANG H, YAO B, GAO X, et al.Effects of glucose oxidase on the growth performance, serum parameters and faecal microflora of piglets[J].South African Journal of Animal Science, 2016, 46(1):14-20.

[9] 穆淑琴, 李宁, 闫峻, 等.葡萄糖氧化酶对仔猪生长性能和血清生化指标的影响[J].中国畜牧兽医, 2018, 45(8):2212-2218. MU S Q, LI N, YAN J, et al.Effect of glucose oxidase on the growth performance and serum biochemical indexes of piglets[J].Chinese Journal Animal Husbandry & Veterinary Medicine, 2018, 45(8):2212-2218.(in Chinese)
[10] WU S R, LI T H, NIU H F, et al.Effects of glucose oxidase on growth performance, gut function, and cecal microbiota of broiler chickens[J].Poultry Science, 2019, 98(2):828-841.

[11] CHOCT M, HUGHES R J, BEDFORD M R.Effects of a xylanase on individual bird variation, starch digestion throughout the intestine, and ileal and caecal volatile fatty acid production in chickens fed wheat[J].British Poultry Science, 1999, 40(3):419-422.

[12] YUAN L, WANG M F, ZHANG X T, et al.Effects of protease and non-starch polysaccharide enzyme on performance, digestive function, activity and gene expression of endogenous enzyme of broilers[J].PLoS One, 2017, 12(3):e0173941.
[13] 张力群, 田涛, 梅桂英.群体感应淬灭——防治植物细菌病害的新策略[J].中国生物防治, 2010, 26(3):241-247. ZHANG L Q, TIAN T, MEI G Y, et al.Quorum quenching, a new strategy for controlling plant bacterial diseases[J].Chinese Journal of Biological Cintrol, 2010, 26(3):241-247.(in Chinese)
[14] 范兴辉, 王惠杉, 何杰华, 等.细菌群体感应淬灭酶及其病害防治研究进展[J].生物技术通报, 2017, 33(10):80-87. FAN X H, WANG H S, HE J H, et al.Research progress on microbial quorum quenching enzymes and their control of plant diseases[J].Biotechnology Bulletin, 2017, 33(10):80-87.(in Chinese)
[15] DONG Y H, WANG L H, XU J L, et al.Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase[J].Nature, 2001, 411(6839):813-817.

[16] CHENG Y H, HSIAO F S H, WEN C M, et al.Mixed fermentation of soybean meal by protease and probiotics and its effects on the growth performance and immune response in broilers[J].Journal of Applied Animal Research, 2019, 47(1):339-348.

[17] 毛银, 陆春波, 李国辉, 等.菌酶协同发酵豆粕工艺的优化[J].食品与发酵工业, 2019, 45(14):108-114. MAO Y, LU C B, LI G H, et al.Optimized fermentation process of soybean meal by bacteria with enzymes[J].Food and Fermentation Industries, 2019, 45(14):108-114.(in Chinese)
[18] 张煜, 石常友, 王成, 等.菌酶协同发酵改善玉米-豆粕型饲料营养价值的研究[J].中国粮油学报, 2018, 33(3):70-77. ZHANG Y, SHI C Y, WANG C, et al.Improving nutrition value of maize-soybean meal based diet by microbial fermentation combined with multiple-enzymes[J].Journal of the Chinese Cereals and Oils Association, 2018, 33(3):70-77.(in Chinese)
[19] 帖余, 李丽, 刘军, 等.菌酶协同处理对发酵菜粕的影响[J].食品与发酵工业, 2019, 45(17):117-122. TIE Y, LI L, LIU J, et al.Effects of cooperation of bacteria and enzyme on fermented rapeseed meal[J].Food and Fermentation Industries, 2019, 45(17):117-122.(in Chinese)
[20] BAI S P, WU A M, DING X M, et al.Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens[J].Poultry Science, 2013, 92(3):663-670.

[21] SARANGI N R, BABU L K, KUMAR A, et al.Effect of dietary supplementation of prebiotic, probiotic, and synbiotic on growth performance and carcass characteristics of broiler chickens[J].Veterinary World, 2016, 9(3):313-319.

[22] SALIM H M, KANG H K, AKTER N, et al.Supplementation of direct-fed microbials as an alternative to antibiotic on growth performance, immune response, cecal microbial population, and ileal morphology of broiler chickens[J].Poultry Science, 2013, 92(8):2084-2090.

[23] SMEETS N, NUYENS F, VAN CAMPENHOUT L, et al.Interactions between the concentration of non-starch polysaccharides in wheat and the addition of an enzyme mixture in a broiler digestibility and performance trial[J].Poultry Science, 2018, 97(6):2064-2070.

[24] MENG X, SLOMINSKI B A, GUENTER W.The effect of fat type, carbohydrase, and lipase addition on growth performance and nutrient utilization of young broilers fed wheat-based diets[J].Poultry Science, 2004, 83(10):1718-1727.

[25] COWIESON A J, ADEOLA O.Carbohydrases, protease, and phytase have an additive beneficial effect in nutritionally marginal diets for broiler chicks[J].Poultry Science, 2005, 84(12):1860-1867.

[26] MASEY O'NEILL H V, LIU N, WANG J P, et al.Effect of xylanase on performance and apparent metabolisable energy in starter broilers fed diets containing one maize variety harvested in different regions of china[J].Asian-Australasian Journal of Animal Sciences, 2012, 25(4):515-523.

[27] AGGER J, VIKSØ-NIELSEN A, MEYER A S.Enzymatic xylose release from pretreated corn bran arabinoxylan:differential effects of deacetylation and deferuloylation on insoluble and soluble substrate fractions[J].Journal of Agricultural and Food Chemistry, 2010, 58(10):6141-6148.

[28] YEGANI M, KORVER D R.Effects of corn source and exogenous enzymes on growth performance and nutrient digestibility in broiler chickens[J].Poultry Science, 2013, 92(5):1208-1220.

[29] FUQUA W C, WINANS S C, GREENBERG E P.Quorum sensing in bacteria:the LuxR-LuxI family of cell density-responsive transcriptional regulators[J].Journal of Bacteriology, 1994, 176(2):269-275.

[30] ALLEN R C, POPAT R, DIGGLE S P, et al.Targeting virulence:can we make evolution-proof drugs?[J].Nature Reviews Microbiology, 2014, 12(4):300-308.

[31] MURUGESAN G R, PERSIA M E.Influence of a direct-fed microbial and xylanase enzyme on the dietary energy uptake efficiency and performance of broiler chickens[J].Journal of the Science of Food and Agriculture, 2015, 95(12):2521-2527.

[32] ASKELSON T E, FLORES C A, DUNN-HORROCKS S L, et al.Effects of direct-fed microorganisms and enzyme blend co-administration on intestinal bacteria in broilers fed diets with or without antibiotics[J].Poultry Science, 2018, 97(1):54-63.

[33] WANG Y Y, WANG Y B, XU H, et al.Direct-fed glucose oxidase and its combination with B. amyloliquefaciens SC06 on growth performance, meat quality, intestinal barrier, antioxidative status, and immunity of yellow-feathered broilers[J].Poultry Science, 2018, 97(10):3540-3549.

[34] 高林, 贾卿, 宋良敏, 等.枯草芽孢杆菌、乳酸菌、酵母菌复合制剂对断奶仔猪生产性能和血清免疫指标的影响[C]//中国畜牧兽医学会动物微生态学分会第十一次全国学术研讨会暨第五届会员代表大会论文集.重庆:中国畜牧兽医学会, 2014:159. GAO L, JIA Q, SONG L M, et al.Effect of B. Subtilis, lactobacillus and yeast compound micro-ecological preparation on weaning piglet reproductive performance and serum immune index[C]//Proceedings of the 11th National Symposium and the 5th Member Representative Conference of Animal Microecology Branch of Chinese Society of Animal Science and Veterinary Medicine.Chongqing:Chinese Association of Animal Science and Veterinary Medicine, 2014, 159.(in Chinese)
[35] 刘辉, 季海峰, 王四新, 等.乳酸片球菌对生长猪生长性能、粪便菌群、血清生化和免疫指标的影响[J].动物营养学报, 2020, 32(6):2558-2566. LIU H, JI H F, WANG S X, et al.Effects of Pediococcus acidilactici on growth performance, fecal microbiota, serum biochemical and immune indexes of growing pigs[J].Chinese Journal of Animal Nutrition, 2020, 32(6):2558-2566.(in Chinese)
[36] 陈瑾, 杨加豹, 邓卉, 等.葡萄糖氧化酶替代饲用抗生素对川藏黑猪配套系商品仔猪生长性能、抗氧化能力及免疫功能的影响[J].饲料工业, 2020, 41(12):11-15. CHEN J, YANG J B, DENG H, et al.Effects of glucose oxidase on growth performance, antioxidant capacity and immune function of Chuanzang black commercial piglets[J].Feed Industry, 2020, 41(12):11-15.(in Chinese)
[37] LI Y H, CHEN X, CHEN Y Q, et al.Effects of β-mannanase expressed by Pichia pastoris in corn-soybean meal diets on broiler performance, nutrient digestibility, energy utilization and immunoglobulin levels[J].Animal Feed Science and Technology, 2010, 159(1):59-67.
[38] FERREIRA H C, Jr, HANNAS M I, ALBINO L F T, et al.Effect of the addition of β-mannanase on the performance, metabolizable energy, amino acid digestibility coefficients, and immune functions of broilers fed different nutritional levels[J].Poultry Science, 2016, 95(8):1848-1857.

[39] ARSENAULT R J, LEE J T, LATHAM R, et al.Changes in immune and metabolic gut response in broilers fed β-mannanase in β-mannan-containing diets[J].Poultry Science, 2017, 96(12):4307-4316.

[40] KIM E Y, MOUDGIL K D.Immunomodulation of autoimmune arthritis by pro-inflammatory cytokines[J].Cytokine, 2017, 98:87-96.
[41] GADDE U, OH S T, LEE Y S, et al.The effects of direct-fed microbial supplementation, as an alternative to antibiotics, on growth performance, intestinal immune status, and epithelial barrier gene expression in broiler chickens[J].Probiotics and Antimicrobial Proteins, 2017, 9(4):397-405.

[42] 任亚雪, 郭乾鹏, 李媛媛, 等.屎肠球菌活菌和热灭活菌对RAW264.7细胞肿瘤坏死因子-α/白细胞介素-10平衡以及丝裂原活化蛋白激酶信号通路的影响[J].动物营养学报, 2020, 32(9):4345-4357. REN Y X, GUO Q P, LI Y Y, et al.Effects of viable and heat-inactivated Enterocuccus faecium on tumor necrosis factor-α/interleukin-10 balance and mitogen-activated protein kinase signaling pathway in RAW264.7 cells[J].Chinese Journal of Animal Nutrition, 2020, 32(9):4345-4357.(in Chinese)
[43] SINGH A, KUKRETI R, SASO L, et al.Oxidative stress:a key modulator in neurodegenerative diseases[J].Molecules, 2019, 24(8):1583.
[44] MURAKAMI A E, RODRIGUEIRO R J B, SANTOS T C, et al.Effects of dietary supplementation of meat-type quail breeders with guanidinoacetic acid on their reproductive parameters and progeny performance[J].Poultry Science, 2014, 93(9):2237-2244.

[45] 王前光, 李闯, 黄璇, 等.葡萄糖氧化酶对1-6周龄临武鸭生长性能、血清生化和抗氧化指标、肠道形态结构和微生物多样性的影响[J].动物营养学报, 2020, 32(8):3605-3614. WANG Q G, LI C, HUANG X, et al.Effects of glucose oxidase on growth performance, serum biochemical and antioxidant indexes, intestinal morphology and microbial diversity of 1 to 6-week-old Linwu ducks[J].Chinese Journal of Animal Nutrition, 2020, 32(8):3605-3614.(in Chinese)
[46] 朱瑾, 匡佑华, 陈继发, 等.枯草芽孢杆菌对肥育猪生长性能、肉品质和抗氧化能力的影响[J].动物营养学报, 2019, 31(6):2572-2578. ZHU J, KUANG Y H, CHEN J F, et al.Effects of Bacillus subtilis on growth performance, meat quality and antioxidant capacity of finishing pigs[J].Chinese Journal of Animal Nutrition, 2019, 31(6):2572-2578.(in Chinese)
[47] 罗波文, 邹田德, 陈丽玲, 等.嗜酸乳杆菌对氧化应激仔猪小肠上皮细胞抗氧化能力和紧密连接蛋白表达的影响[J].动物营养学报, 2020, 32(5):2108-2115. LUO B W, ZOU T D, CHEN L L, et al.Effects of Lactobacillus acidophilus on antioxidative ability and expression of tight junction protein in intestinal epithelial cells of piglets under oxidative stress[J].Chinese Journal of Animal Nutrition, 2020, 32(5):2108-2115.(in Chinese)
[48] NIAN F, GUO Y M, RU Y J, et al.Effect of exogenous xylanase supplementation on the performance, net energy and gut microflora of broiler chickens fed wheat-based diets[J].Asian-Australasian Journal of Animal Sciences, 2011, 24(3):400-406.

[49] 任美琦, 颜瑞, 安文俊, 等.小麦日粮中添加NSP酶对肉鸭生长性能及血清生化指标的影响[J].畜牧与兽医, 2011, 43(1):53-56. REN M Q, YAN R, AN W J, et al.Effects of NSP enzyme supplementation in wheat diets on growth performance and serum biochemical indices of meat ducks[J].Animal Husbandry & Veterinary Medicine, 2011, 43(1):53-56.(in Chinese)
[50] SAKI A A, MIRZAYI S, HARSINI S G, et al.Effect of various level of treated barley on small intestinal content viscosity, litter moisture, uric acid and broiler chickens performance[J].Journal of Animal and Veterinary Advances, 2010, 8(3):825-836.
[51] 郑爱娟, 吴正可, AHMED P S, 等.丁酸梭菌对肉鸡生长性能和血清生化指标的影响[J].动物营养学报, 2019, 31(12):5519-5525. ZHENG A J, WU Z K, AHMED P S, et al.Effects of Clostridium butyricum on growth performance and serum biochemical indices of broilers[J].Chinese Journal of Animal Nutrition, 2019, 31(12):5519-5525.(in Chinese)

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