Effects of Lutein on Growth Performance, Jejunum Morphology and Cecal Microorganisms of Lipopolysaccharide-Stimulated Yellow-Feathered Broilers

WANG Meiyan; TONG Yuxin; MIN Yao; WANG Changkang; GAO Yuyun

doi:10.3969/j.issn.1006-267x.2021.10.017

2021 , Vol. 33 >Issue 10: 5569 - 5580

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.017

POULTRY NUTRITION AND FEED

Effects of Lutein on Growth Performance, Jejunum Morphology and Cecal Microorganisms of Lipopolysaccharide-Stimulated Yellow-Feathered Broilers

WANG Meiyan ,
TONG Yuxin ,
MIN Yao ,
WANG Changkang ,
GAO Yuyun

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College of Animal Science/College of Bee Science, Fujian Agricultural and Forestry University, Fuzhou 350002, China

Received date: 2021-03-11

Online published: 2021-10-16

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Abstract

This experiment aimed to study the effects of different levels of lutein on growth performance, intestinal morphology and cecal microorganisms of lipopolysaccharide (LPS)-stimulated yellow-feather broilers. Two hundred eighty-eight one-day-old healthy yellow-feathered male broilers with similar body weight were randomly divided into 3 groups with 8 replicates per group and 12 broilers per replicate. Broilers in control group were fed a basal diet, and the others in experimental groups were fed the basal diets supplemented with 20 and 40 mg/kg lutein. Three consecutive intraperitoneal injections of 1 mg/kg LPS were given to broilers at 21, 23 and 25 days of age. The experimental period lasted for 26 days. The results showed as follows:1) compared with control group, dietary 40 mg/kg lutein significantly increased average daily gain of LPS-stimulated yellow-feathered broilers from 21 to 26 days of age and 1 to 26 days of age (P<0.05), and significantly decreased the ratio of feed to gain of broilers from 22 to 26 days of age (P<0.05). 2) Compared with control group, dietary 20 and 40 mg/kg lutein significantly increased villus height of jejunum of LPS-stimulated yellow-feathered broilers (P<0.05), and dietary 40 mg/kg lutein significantly increased the ratio of villus height to crypt depth (V/C) of jejunum (P<0.05). 3) Compared with control group, dietary 20 and 40 mg/kg lutein significantly increased the Shannon index of cecal microorganism of LPS-stimulated yellow-feathered broilers (P<0.05); and the microflora structure was similar, but the distance to control group was far. Compared with control group, dietary 20 and 40 mg/kg lutein significantly increased relative abundance of Firmicutes and Eisenbergiella (P<0.05), and significantly decreased relative abundance of Proteobacteria and unidentified Enterobacteriaceae (P<0.05); and Kyoto Encyclopedia of genes and genomes (KEGG) pathways related to amino acid, carbohydrate metabolism and genetic information processing were significantly enriched in 20 and 40 mg/kg lutein groups (P<0.05). In conclusion, dietary 20 and 40 mg/kg lutein can improve jejunal morphology and cecal microflora composition of LPS-stimulated yellow-feathered broilers, and 40 mg/kg lutein has the best growth promoting effect.

Key words： lutein; LPS; broilers; growth performance; jejunum morphology; cecal microorganisms

Cite this article

WANG Meiyan , TONG Yuxin , MIN Yao , WANG Changkang , GAO Yuyun . Effects of Lutein on Growth Performance, Jejunum Morphology and Cecal Microorganisms of Lipopolysaccharide-Stimulated Yellow-Feathered Broilers[J]. Chinese Journal of Animal Nutrition, 2021 , 33(10) : 5569 -5580 . DOI: 10.3969/j.issn.1006-267x.2021.10.017

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