Effects of Antredia cinnamomea Polysaccharide on Growth Performance and Jejunal Mucosa Integrity of Yellow-Feathered Chickens Challenged with Lipopolysaccharide

YE Jinling; FAN Qiuli; LIN Xiajing; WANG Yibing; ZHANG Sheng; GOU Zhongyong; JIANG Shouqun

doi:10.3969/j.issn.1006-267x.2021.10.020

2021 , Vol. 33 >Issue 10: 5601 - 5616

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.020

POULTRY NUTRITION AND FEED

Effects of Antredia cinnamomea Polysaccharide on Growth Performance and Jejunal Mucosa Integrity of Yellow-Feathered Chickens Challenged with Lipopolysaccharide

YE Jinling ,
FAN Qiuli ,
LIN Xiajing ,
WANG Yibing ,
ZHANG Sheng ,
GOU Zhongyong ,
JIANG Shouqun

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Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2021-04-01

Online published: 2021-10-16

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Abstract

This experiment was conducted to investigate the effects of Antredia cinnamomea polysaccharide (ACP) on growth performance and jejunal mucosa integrity of yellow-feathered chickens challenged with lipopolysaccharide (LPS). And the mechanism was analyzed by comparing the effects of Astragalus polysaccharide (APS) and algae-derived polysaccharide (ADP). According to the principle of consistent body weight, 1 200 healthy 9-day-old fast-large type Lingnan yellow-feathered chickens (female) were divided into 8 groups (6 replicates per group and 25 chickens per replicate) which were blank control group (basal diet), LPS stress group (basal diet+LPS stress), antibiotics group (basal diet+LPS stress+20 mg/kg virginiamycin), 100 mg/kg ACP group (basal diet+LPS stress+100 mg/kg ACP), 200 mg/kg ACP group (basal diet+LPS stress+200 mg/kg ACP), 400 mg/kg ACP group (basal diet+LPS stress+400 mg/kg ACP), 400 mg/kg APS group (basal diet+LPS stress+400 mg/kg APS) and 200 mg/kg ADP group (basal diet+LPS stress+200 mg/kg ADP), respectively. At 18 and 20 days of age, each chicken in the blank control group was intraperitoneally injected with 0.50 mL of normal saline, and in the other groups were intraperitoneally injected with 0.50 mL of LPS (500 μg/kg BW), respectively. The experiment lasted for 21 days. The results showed as follows:1) compared with the blank control group, LPS stress significantly decreased the growth performance of chickens (P<0.05); compared with the LPS stress group, the average daily feed intake of chickens in the other groups was significantly increased (P<0.05), and the average daily gain of chickens in 200 mg/kg ADP group was significantly increased (P<0.05); compared with the antibiotics group, different dietary polysaccharides had no significant effects on growth performance of chickens (P<0.05). 2) Compared with the LPS stress group and antibiotics group, dietary 200 and 400 mg/kg ACP significantly increased the glutathione peroxidase activity in plasma and jejunal mucosa of chickens (P<0.05); compared with the LPS stress group, the malondialdehyde content in plasma and jejunal mucosa of chickens in ACP groups was significantly decreased (P<0.05). 3) Compared with the LPS stress group, dietary ACP and APS significantly decreased the contents of tumor necrosis factor-α (TNF-α) and interferon-γ in plasma of chickens (P<0.05), and significantly decreased the contents of TNF-α, interleukin-1β (IL-1β) and interleukin-6 in jejunal mucosa of chickens (P<0.05). However, only dietary 100 mg/kg ACP significantly increased the interleukin-10 (IL-10) content of in jejunal mucosa (P<0.05). 4) Compared with the LPS stress group, the jejunal villus height of chickens in ACP groups and 400 mg/kg APS group was significantly increased (P<0.05), the crypt depth was significantly decreased (P<0.05), the villus height to crypt depth ratio was significantly increased (P<0.05), and the intestinal wall thickness was also significantly decreased (P<0.05). Compared with the antibiotics group, the jejunal villus height in ACP and APS groups had no significant change (P<0.05), but the crypt depth, intestinal wall thickness and villus height to crypt depth ratio were significantly improved (P<0.05). Compared with ACP groups and 400 mg/kg APS group, the structural integrity of jejunal mucosa of chickens in 200 mg/kg ADP group was worse. 5) Compared with the LPS stress group and antibiotics group, the mRNA relative expression levels of Toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB) and IL-1β in jejunum of chickens in ACP groups and 400 mg/kg APS group were significantly decreased (P<0.05). Compared with the LPS stress group, the mRNA relative expression levels of mucin 2 (MUC2), claudin-1, zonula occludens-1 (ZO-1) and occludin in jejunum in ACP groups were significantly increased (P<0.05). The mRNA relative expression levels of MUC2, claudin-1 and occludin in jejunum in 400 mg/kg APS group were significantly increased (P<0.05), and the claudin-1 mRNA relative expression level in jejunum in 200 mg/kg ADP group was significantly increased (P<0.05). Compared with the antibiotics group, dietary 200 and 400 mg/kg ACP significantly increased the mRNA relative expression levels of MUC2, claudin-1 and occludin in jejunum (P<0.05). In conclusion, the basal diet supplemented with ACP for fast-large type Lingnan yellow-feathered chickens can effectively alleviate the growth inhibition, oxidative damage and intestinal structure damage induced by LPS, and the effect is better than that of antibiotics. The mechanism of action may be similar to that of APS, that is, by inhibiting the expression of TLR4/NF-κB induced by LPS, then the inflammatory response can be inhibited, the antioxidant capacity of the body can be improved, and the intestinal mucosa barrier function can be enhanced, so as to fully play the function of repairing inflammatory injury. As ACP is not dose-dependent, 100 mg/kg is recommended.

Key words： Antredia cinnamomea polysaccharide; yellow-feathered chickens; LPS; growth performance; jejunal mucosa

Cite this article

YE Jinling , FAN Qiuli , LIN Xiajing , WANG Yibing , ZHANG Sheng , GOU Zhongyong , JIANG Shouqun . Effects of Antredia cinnamomea Polysaccharide on Growth Performance and Jejunal Mucosa Integrity of Yellow-Feathered Chickens Challenged with Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2021 , 33(10) : 5601 -5616 . DOI: 10.3969/j.issn.1006-267x.2021.10.020

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