Effects of Glucose Oxidase on Laying Performance, Hatching Performance and Antioxidant Capacity of Laying Breeders during Later Laying Period

LI Jiahui; LI Shengjie; GONG Jiangang; JING Jialin; HAO Yanshuang; FENG Zhihua; ZHAO Guoxian

doi:10.3969/j.issn.1006-267x.2021.10.021

2021 , Vol. 33 >Issue 10: 5617 - 5626

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.021

POULTRY NUTRITION AND FEED

Effects of Glucose Oxidase on Laying Performance, Hatching Performance and Antioxidant Capacity of Laying Breeders during Later Laying Period

LI Jiahui ,
LI Shengjie ,
GONG Jiangang ,
JING Jialin ,
HAO Yanshuang ,
FENG Zhihua ,
ZHAO Guoxian

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1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001, China;
2. Hefei Zhengda Co., Ltd., Hefei 230601, China;
3. College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China

Received date: 2021-03-18

Online published: 2021-10-16

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Abstract

The purpose of this study was to investigate the effects of glucose oxidase (GOD) on laying performance, hatching performance, antioxidant capacity and related genes expression of laying breeders during later laying period. Using single factor experimental design, four hundred and eighty 55-week-old Hyline brown laying breeders with similar body weight and laying rate were randomly divided into 4 groups with 6 replicates per group and 20 hens per replicate. Laying breeders in control group were fed a basal diet, and the other in experimental groups were fed the basal diets supplemented with 200, 350 and 500 mg/kg GOD, respectively. The pretest lasted for 1 week and the trial lasted for 12 weeks. The results showed as follows:1) compared with control group, laying rate of laying breeders during later laying period in 350 and 500 mg/kg GOD groups was significantly increased (P<0.05), but the ratio of feed to egg was significantly decreased (P<0.05). Compared with control group, the fertilization rate of breeding eggs in 200, 350 and 500 mg/kg GOD groups was significantly increased (P<0.05), and there were no significant differences in hatching rate and birth rate of breeding eggs (P>0.05). 2) Compared with control group, eggshell thickness and Haugh unit of breeding eggs of laying breeders during later laying period in 350 and 500 mg/kg GOD groups were significantly increased (P<0.05). 3) Compared with control group, the content of malonaldehyde (MDA) in serum of laying breeders during later laying period in 500 mg/kg GOD group was significantly decreased (P<0.05), the activity of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in serum in 200, 350 and 500 mg/kg GOD groups were significantly increased (P<0.05), and the activity of glutathione peroxidase (GSH-Px) in serum in 350 and 500 mg/kg GOD groups was significantly increased (P<0.05). 4) Compared with control group, the content of MDA in liver of laying breeders during later laying period in 200, 350 and 500 mg/kg GOD groups was significantly decreased and the activity of SOD in liver was significantly increased (P<0.05), and the activity of GSH-Px in liver in 350 and 500 mg/kg GOD groups was significantly increased (P<0.05). 5) Compared with control group, the mRNA relative expression levels of nuclear factor E2 related factor 2 (Nrf2), manganese superoxide dismutase (Mn-SOD) and copper zinc superoxide dismutase (Cu/Zn-SOD) of laying breeders during later laying period in 200, 350 and 500 mg/kg GOD groups were significantly increased (P<0.05). 6) Dietary 500 mg/kg GOD had the best economic benefit. In conclusion, dietary GOD may improve laying performance, hatching performance and egg quality of laying breeders during later laying period by activating related genes expression of Nrf2/antioxidant reaction element (ARE) signaling pathway in liver cells and improving antioxidant capacity in serum and liver. Comprehensive economic benefit analysis shows that the appropriate supplemental level of GOD in diets of laying breeders during later laying period is 500 mg/kg under the experimental conditions.

Key words： glucose oxidase; later laying period; laying breeders; laying performance; hatching performance; antioxidant capacity; mRNA expression

Cite this article

LI Jiahui , LI Shengjie , GONG Jiangang , JING Jialin , HAO Yanshuang , FENG Zhihua , ZHAO Guoxian . Effects of Glucose Oxidase on Laying Performance, Hatching Performance and Antioxidant Capacity of Laying Breeders during Later Laying Period[J]. Chinese Journal of Animal Nutrition, 2021 , 33(10) : 5617 -5626 . DOI: 10.3969/j.issn.1006-267x.2021.10.021

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