Correlation between Content of Odd- and Branched-Chain Fatty Acids in Rumen and Milk and Production of Microbial Protein in Rumen

LIU Xin; JIANG Xin; XU Hongjian; ZHANG Shuzhi; GUAN Jiaqi; XIN Hangshu

doi:10.3969/j.issn.1006-267x.2021.10.031

2021 , Vol. 33 >Issue 10: 5717 - 5728

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.031

RUMINANT AND HERBIVORE NUTRITION AND FEED

Correlation between Content of Odd- and Branched-Chain Fatty Acids in Rumen and Milk and Production of Microbial Protein in Rumen

LIU Xin ,
JIANG Xin ,
XU Hongjian ,
ZHANG Shuzhi ,
GUAN Jiaqi ,
XIN Hangshu

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1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
2. Fuyu Hefeng Feed Co., Ltd., Fuyu 131200, China;
3. Shenyang Hefeng Ruminant Feed Co., Ltd., Shenyang 110164, China;
4. College of Animal Science and Medicine, Shenyang Agricultural University, Shenyang 110161, China

Received date: 2021-03-18

Online published: 2021-10-16

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Abstract

This experiment was conducted to explore correlation between odd-chain branched fatty acid (OBCFA) content and rumen microbial protein (MCP) production in rumen and milk. Three Holstein lactating cows[parity=1; lactation days=(142.0±15.8) d; milk yield=(27.0±6.2) kg/d] with permanent fistulas were selected in the present experiment. The 3×3 Latin square test design was used to infuse three doses of NH₄HCO₃ (0, 112 and 224 g/d) into the rumen. The pH, volatile fatty acids (VFA), ammonia nitrogen (NH₃-N), MCP, OBCFA contents in rumen, and OBCFA content in milk were measured. The results showed that with the increase of NH₄HCO₃ dose, the contents of iso-C14:0, iso-C17:0 and total isometeric fatty acids in rumen were significantly increased (P<0.05), and the MCP content tended to be increased (P=0.06); the content of anteiso-C15:0 tended to increase with the increase of NH₄HCO₃ dose (P=0.07). By correlation analysis, it was found that the OBCFA content in rumen were related to ruminal pH, NH₃-N, acetate, propionate, butyrate, isovalerate, valerate and TVFA contents (r=0.26 to 0.64, P<0.05); the OBCFA content in milk were related to rumen pH, NH₃-N, acetate, butyrate and valerate contents (r=0.71 to 0.84, P<0.05); the ruminal MCP content was positively correlated with anteiso-C15:0 (r=0.79), anteiso-C17:0 (r=0.76) and TAFA (r=0.84) contents in the rumen (P<0.05), and there was a significant positive correlation with anteiso-C15:0 (r=0.78) content in milk (P<0.05). In summary, OBCFA content in the rumen and milk can reflect changes in rumen fermentation parameters, and anteiso-C15:0 and anteiso-C17:0 contents in the rumen and anteiso-C15:0 content in milk can be used as markers to predict the ruminal MCP content.

Key words： OBCFA; MCP; rumen fermentation; correlation

Cite this article

LIU Xin , JIANG Xin , XU Hongjian , ZHANG Shuzhi , GUAN Jiaqi , XIN Hangshu . Correlation between Content of Odd- and Branched-Chain Fatty Acids in Rumen and Milk and Production of Microbial Protein in Rumen[J]. Chinese Journal of Animal Nutrition, 2021 , 33(10) : 5717 -5728 . DOI: 10.3969/j.issn.1006-267x.2021.10.031

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