FENG Guanzhi , XU Hongjian , WANG Lihua , ZHANG Chengrui , ZU Zhifu , ZHANG Yonggen . Effects of 25-Hydroxyvitamin D₃ and Vitamin D₃ on Semen Quality and Serum Biochemical Indices of Holstein Bulls[J]. Chinese Journal of Animal Nutrition, 2021 , 33(10) : 5729 -5737 . DOI: 10.3969/j.issn.1006-267x.2021.10.032

[1] AL-MUTARY M G, AL-GHADI M Q, AMMARI A A, et al.Effect of different concentrations of resveratrol on the quality and in vitro fertilizing ability of ram semen stored at 5 C for up to 168 h[J].Theriogenology, 2020, 152:139-146.
[2] 徐宏建, 李昕, 王丽华, 等.25羟基维生素D₃的生理功能及其在动物生产中的应用[J].动物营养学报, 2020, 32(4):1491-1498. XU H J, LI X, WANG L H, et al.Physiological function of 25-hydroxyvitamin D₃ and its application in animal production[J].Chinese Journal of Animal Nutrition, 2020, 32(4):1491-1498.(in Chinese)
[3] 卢娜, 宗学醒, 王雅晶, 等.不同类型维生素D₃对奶牛产奶性能, 血液指标及钙磷代谢的影响[J].动物营养学报, 2018, 30(8):2997-3004. LU N, ZONG X X, WANG Y J, et al.Effects of different types of vitamin D₃ on milk production, blood indices, calcium and phosphorus metabolism of dairy cows[J].Chinese Journal of Animal Nutrition, 2018, 30(8):2997-3004.(in Chinese)
[4] 董洪俊.饲粮添加不同水平及形式的维生素D对公猪繁殖性能的影响[D].硕士学位论文.成都:四川农业大学, 2016. DONG H J.Effects of different vitamin D levels and forms on boar reproduction[D].Master's Thesis.Chengdu:Sichuan Agricultural University, 2016.(in Chinese)
[5] HORST R L, GOFF J P, REINHARDT T A.Calcium and vitamin D metabolism in the dairy cow[J].Journal of Dairy Science, 1994, 77(7):1936-1951.  
[6] 曹令勤, 于朝辉, 张文馨, 等.25-羟基维生素D₃添加时间对清远麻鸡种公鸡精液质量的影响[J].家禽科学, 2020(9):8-10. CAO L Q, YU C H, ZHANG W X, et al.Effect of supplementation time of 25-hydroxyl vitamin D₃ semen quality of flax seed cock[J].Poultry Science, 2020(9):8-10.(in Chinese)
[7] LIU Q, ZHOU Y F, DUAN R J, et al.Effects of dietary n-6:n-3 fatty acid ratio and vitamin E on semen quality, fatty acid composition and antioxidant status in boars[J].Animal Reproduction Science, 2015, 162:11-19.
[8] HAFEZ B, HAFEZ E S E.Reproduction in farm animals[M].7th ed.Lippincott:Williams & Wilkins, 2000.
[9] MACFARLANE J S.Salamon's artificial insemination of sheep and goats[J].Tropical Animal Health and Production, 1991, 23(2):114.
[10] CHINOY N J, RANGA G M, HIGHLAND H N, et al.A modified method for the differential staining of spermatozoa using alcoholic acidic silver nitrate[J].International Journal of Fertility, 1992, 37(4):232-236.
[11] REVELL S G, MRODE R A.An osmotic resistance test for bovine semen[J].Animal Reproduction Science, 1994, 36(1/2):77-86.
[12] MARTINEZ N, RISCO C A, LIMA F S, et al.Evaluation of peripartal calcium status, energetic profile, and neutrophil function in dairy cows at low or high risk of developing uterine disease[J].Journal of Dairy Science, 2012, 95(12):7158-7172.  
[13] BOLTZ D F, MELLON M G.Spectrophotometric determination of phosphorus as molybdiphosphoric acid[J].Analytical Chemistry, 2002, 20(8):749-751.
[14] ARAB A, HADI A, MOOSAVIAN S P, et al.The association between serum vitamin D, fertility and semen quality:a systematic review and meta-analysis[J].International Journal of Surgery, 2019, 71:101-109.
[15] BEDU-ADDO K, COSTELLO S, HARPER C, et al.Mobilisation of stored calcium in the neck region of human sperm-a mechanism for regulation of flagellar activity[J].The International Journal of Developmental Biology, 2008, 52(5/6):615-626.
[16] 李亮亮, 于德新, 谢栋栋, 等.吸烟对雄性小鼠睾丸间质细胞、睾酮及精子数量的影响[J].中国男科学杂志, 2015, 29(3):23-28. LI L L, YU D X, XIE D D, et al.Effects of smoking on leydig cells, testosterone levels and sperm counts of male mice[J].Chinese Journal of Andrology, 2015, 29(3):23-28.(in Chinese)
[17] MERINO O, SÁNCHEZ R, GREGORIO M B, et al.Effect of high-fat and vitamin D deficient diet on rat sperm quality and fertility[J].Theriogenology, 2019, 125:6-11.
[18] LIN Y, LV G, DONG H J, et al.Effects of the different levels of dietary vitamin D on boar performance and semen quality[J].Livestock Science, 2017, 203:63-68.
[19] ROLDAN E S R.Assessments of sperm quality integrating morphology, swimming patterns, bioenergetics and cell signalling[J].Theriogenology, 2020, 150:388-395.
[20] MUKHOPADHYAY S, SINGH M, CHATTERJEE M.Vitamin D₃ as a modulator of cellular antioxidant defence in murine lymphoma[J].Nutrition Research, 2000, 20(1):91-102.  
[21] BLOMBERG JENSEN M.Vitamin D and male reproduction[J].Nature Reviews Endocrinology, 2014, 10(3):175-186.  
[22] MARTINEZ N, RODNEY R M, BLOCK E, et al.Effects of prepartum dietary cation-anion difference and source of vitamin D in dairy cows:health and reproductive responses[J].Journal of Dairy Science, 2018, 101(3):2563-2578.  
[23] CELI P, WILLIAMS S, ENGSTROM M, et al.Safety evaluation of dietary levels of 25-hydroxyvitamin D₃ in growing calves[J].Food and Chemical Toxicology, 2018, 111:641-649.
[24] BIKLE D D.Vitamin D metabolism, mechanism of action, and clinical applications[J].Chemistry & Biology, 2014, 21(3):319-329.  
[25] 孙彬.1α-羟基维生素D₃原及25-羟基维生素D₃的合成[D].博士学位论文.杭州:浙江工业大学, 2016. SUN B.Synthesis of 1α-hydroxyprovitamin D₃ and 25-hydroxyprovitamin D₃[D].Ph.D.Thesis.Hangzhou:Zhejiang University of Techonology, 2016.(in Chinese)
[26] HOFER D, MVNZKER J, SCHWETZ V, et al.Testicular synthesis and vitamin D action[J].The Journal of Clinical Endocrinology and Metabolism, 2014, 99(10):3766-3773.  
[27] KINUTA K, TANAKA H, MORIWAKE T, et al.Vitamin D is an important factor in estrogen biosynthesis of both female and male gonads[J].Endocrinology, 2000, 141(4):1317-1324.  
[28] WEERAKOON W, SAKASE M, KOHAMA N, et al.Plasma estradiol-17β, cortisol, and insulin concentrations and serum biochemical parameters surrounding puberty in Japanese Black beef bulls with normal and abnormal semen[J].Theriogenology, 2020, 148:18-26.
[29] DEVKOTA B, KOSEKI T, MATSUI M, et al.Relationships among age, body weight, scrotal circumference, semen quality and peripheral testosterone and estradiol concentrations in pubertal and postpubertal Holstein bulls[J].The Journal of Veterinary Medical Science, 2008, 70(1):119-121.  
[30] MOURA A A, SOUZA C E A, ERICKSON B H.Early prepubertal testis criteria, seminiferous epithelium and hormone concentrations as related to testicular development in beef bulls[J].Animal Reproduction Science, 2011, 124(1/2):39-47.
[31] 王可宝.饲料中不同水平维生素D₃对团头鲂生产性能、非特异性免疫及抗病原菌感染的影响[D].硕士学位论文.南京:南京农业大学, 2011. WANG K B.Effects of dietary vitamin D₃ levels on growth, non-specific immunity and anti-pathogenic of Wuchang bream (Megalobrama amblycephala, yih)[D].Master's Thesis.Nanjing:Nanjing Agricultural University, 2011.(in Chinese)
[32] JOHNSON B H, WELSH T J, Jr, JUNIEWICZ P E.Suppression of luteinizing hormone and testosterone secretion in bulls following adrenocorticotropin hormone treatment[J].Biology of Reproduction, 1982, 26(2):305-310.  
[33] 王雍, 杨伟月, 马晓晓, 等.运输应激对肉牛血清中多种应激指标的影响[J].黑龙江畜牧兽医, 2018(4):1-3. WANG Y, YANG W Y, MA X X, et al.The effect of transport stress on multiple stress indicators in beef serum[J].Heilongjiang Animal Science and Veterinary Medicine, 2018(4):1-3.(in Chinese)
[34] 张淑云, 王安.钙和维生素D对生长肉鸡免疫及抗氧化功能的影响[J].动物营养学报, 2010, 22(3):579-585. ZHANG S Y, WANG A.Effects of calcium and vitamin D on immune and antioxidant functions in growing broilers[J].Chinese Journal of Animal Nutrition, 2010, 22(3):579-585.(in Chinese)
[35] YANG J W, TIAN G, CHEN D W, et al.Effects of dietary 25-hydroxyvitamin D₃ supplementation on growth performance, immune function and antioxidative capacity in weaned piglets[J].Archives of Animal Nutrition, 2019, 73(1):44-51.  
[36] WISEMAN H.Vitamin D is a membrane antioxidant.Ability to inhibit iron-dependent lipid peroxidation in liposomes compared to cholesterol, ergosterol and tamoxifen and relevance to anticancer action[J].FEBS Letters, 1993, 326(1/2/3):285-288.