Effects of Plant Extract Mixture on Free Amino Acids Content and Relative Expression Level of Their Transporters in Gastrointestinal Tract of Xiangdong Black Goat

XU Qixiang; CAO Wenhao; LUO Shuanggui; XIE Jingjing; LYU Xiaokang; ZHU Jinjia; WU Yicheng; ZHOU Chuanshe; TAN Zhiliang

doi:10.3969/j.issn.1006-267x.2021.10.035

2021 , Vol. 33 >Issue 10: 5760 - 5770

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.10.035

RUMINANT AND HERBIVORE NUTRITION AND FEED

Effects of Plant Extract Mixture on Free Amino Acids Content and Relative Expression Level of Their Transporters in Gastrointestinal Tract of Xiangdong Black Goat

XU Qixiang ,
CAO Wenhao ,
LUO Shuanggui ,
XIE Jingjing ,
LYU Xiaokang ,
ZHU Jinjia ,
WU Yicheng ,
ZHOU Chuanshe ,
TAN Zhiliang

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1. Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Science, Changsha 410125, China;
2. College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;
3. Hunan Xianwei Industrial Co., Ltd., Changsha 410016, China

Received date: 2021-03-05

Online published: 2021-10-16

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Abstract

The study was conducted to explore the effects of plant extract mixture (PEM) (Eucommia ulmoides, rosemary, loquat and persimmon leaves) on free amino acids contents and relative expression levels of amino acid transporters in the rumen and intestine of Xiangdong black goats. In total, twenty healthy female-goats with similar weight of (9.82±0.83) kg were selected and equally randomly assigned into 2 groups, control group and experimental group (basic diets with 0.15% PEM). The experiment lasted for 56 days. The results showed that compared with control group, 1) adding PEM to the diet could significantly increase the rumen free aspartic acid (Asp), glutamic acid (Glu), glycine (Gly), alanine (Ala) and proline (Pro) contents of Xiangdong black goats (P<0.01), the content of free threonine (Thr) was significantly increased (P<0.05); the relative expression level of rumen mucosal calcium-sensing receptor (CASR) gene was significantly up-regulated by 68.6% (P<0.01); 2) adding PEM to the diet could significantly increase the contents of free Gly, Ala and arginine (Arg) in the ileum of Xiangdong black goats (P<0.01) and significantly increase the content of free valine (Val) (P<0.05); the relative expression level of ileal mucosal solute carrier family 1 member 1 (SLC1A1) gene was significantly up-regulated by 162.6% (P<0.01), the relative expression levels of solute carrier family 1 member 3 (<SLC1A3) and solute carrier family 38 member 2 (SLC38A2) genes were significantly increased by 27.1% and 34.3% (P<0.05), respectively; 3) adding PEM to the diet could significantly reduce the cecal free Thr, Glu, Gly, tyrosine (Tyr) and phenylalanine (Phe) contents (P<0.01), and at the same time, could significantly reduce serine (Ser) and Ala (P<0.05); the relative expression level of SLC1A1 gene in cecal mucosa was significantly down-regulated by 77.2% (P<0.01), and the relative expression level of solute carrier family 7 member 1 (SLC7A1) gene was significantly up-regulated by 29.2% (P<0.01); the relative expression level of solute carrier family 3 member 2 (SLC3A2) and SLC38A2 genes were significantly up-regulated by 21.5% and 34.3% (P<0.05). In summary, the PEM can significantly increase the contents of free amino acids in the rumen and intestines of Xiangdong black goats, while regulating the relative expression of amino acid transporters in the rumen and intestinal mucosa.

Key words： Xiangdong black goat; plant extract mixture; free amino acid; amino acid transporter

Cite this article

XU Qixiang , CAO Wenhao , LUO Shuanggui , XIE Jingjing , LYU Xiaokang , ZHU Jinjia , WU Yicheng , ZHOU Chuanshe , TAN Zhiliang . Effects of Plant Extract Mixture on Free Amino Acids Content and Relative Expression Level of Their Transporters in Gastrointestinal Tract of Xiangdong Black Goat[J]. Chinese Journal of Animal Nutrition, 2021 , 33(10) : 5760 -5770 . DOI: 10.3969/j.issn.1006-267x.2021.10.035

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