EXPERIMENTAL METHOD AND ANIMAL

Effects of Vegetable Carbon Black on Growth Performance and Reproductive System of Rats Fed Zearalenone Contaminated Diet

  • LIU Shujie ,
  • DENG Bo ,
  • WU Jie ,
  • XU Ziwei ,
  • MA Qianqian
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  • Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received date: 2021-03-20

  Online published: 2021-10-16

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Abstract

In this study, the effects of different doses of dietary vegetable carbon black in the zearalenone (ZEN) contaminated diet on the growth performance and reproductive system of rats were studied, so as to evaluate the adsorption effects of vegetable carbon black on ZEN and the improvement effects on the health of rats, and to provide a basis for its use in animal husbandry and feed industry. In a completely randomized design, sixty-four 5- to 6-week-old specific pathogen free (SPF) female SD rats with a body weight of 150 g were randomly divided into 4 groups with 16 replicates per group and 1 rat per replicate. The four groups were as follows:1) the blank control group, and the rats were fed a basal diet; 2) the negative control group, and the rats were fed the basal diet+25 mg/kg ZEN; 3) the low-dose vegetable carbon black group, and the rats were fed the basal diet+25 mg/kg ZEN+1.0 g/kg vegetable carbon black; 4) the high-dose vegetable carbon black group, and the rats were fed the basal diet+25 mg/kg ZEN+1.5 g/kg vegetable carbon black. The experimental period lasted for 28 days after 7-day adaption. The results showed as follows:1) compared with the blank control group, the average daily gain (ADG) of rats in the negative control group was significantly decreased by 26.06% from days 1 to 14 (P<0.05), and was extremely significantly decreased by 28.45% from days 1 to 28 (P<0.01); the ratio of feed to gain (F/G) in the negative control group was significantly increased by 33.08% from days 1 to 14 (P<0.05), and was extremely significantly increased by 28.21% and 40.73% from days 15 to 28 and days 1 to 28 (P<0.01), respectively. Compared with the negative control group, the F/G of rats in the high-dose vegetable carbon black group was significantly decreased by 15.90% and 20.89% from days 15 to 28 and days 1 to 28 (P<0.05), respectively. 2) Compared with the blank control group, the serum alkaline phosphatase (ALP) activity of rats in the negative control group was extremely significantly increased by 37.67% and 44.17% on days 14 and 28 (P<0.01), the serum glutamic-pyruvic transaminase (GPT) activity was significantly increased by 42.55% on day 28 (P<0.05), and the serum glutamic-oxalacetic transaminase (GOT) activity was extremely significantly increased by 37.75% on day 28 (P<0.01). Compared with the negative control group, the serum ALP activity of rats in the high-dose vegetable carbon black group was extremely significantly decreased by 22.48% on day 14 (P<0.01), and the serum GPT and GOT activities were significantly decreased by 24.48% and 20.27% on day 28 (P<0.05), respectively. 3) Compared with the blank control group, the serum follicle stimulating hormone (FSH) concentration of rats in the negative control group was extremely significantly increased by 20.40% on day 14 (P<0.01), and was significantly increased by 19.86% on day 28 (P<0.05); the serum estradiol (E2) concentration of rats in the negative control group was extremely significantly decreased by 19.30% and 17.59% on days 14 and 28 (P<0.01); the serum luteinizing hormone (LH) concentration of rats in the negative control group was extremely significantly decreased by 46.74% on day 14 (P<0.01), and was significantly decreased by 25.63% on day 28 (P<0.05). Compared with the negative control group, the serum LH concentration of rats in the low-dose vegetable carbon black group was extremely significantly increased by 35.20% on day 14 (P<0.01); the serum FSH concentration in the high-dose vegetable carbon black group was extremely significantly decreased by 28.94% on day 14 (P<0.01), and was significantly decreased by 19.38% on day 28 (P<0.05); the serum E2 concentration of rats in high-dose vegetable carbon black group was extremely significantly increased by 29.98% and 31.50% on days 14 and 28 (P<0.01), and the serum LH concentration was extremely significantly increased by 71.94% on day 14 (P<0.01). 4) Hematoxylin-eosin (HE) staining of the ovarian tissue showed that atrophy was observed in the ovarian cortex in the negative control group, and the numbers of primordial follicles, primary ovarian follicles and primary oocytes were significantly decreased compared with the blank control group. The morphology of the ovarian tissue in the low-dose and high-dose of vegetable carbon black groups was significantly improved compared with the negative control group. In conclusion, the vegetable carbon black has the ability to adsorb ZEN, and dietary plant carbon black can alleviate the growth performance decline and reproductive system disorder caused by ZEN in rats. The recommended dosage of vegetable carbon black is 1.5 g/kg.

Cite this article

LIU Shujie , DENG Bo , WU Jie , XU Ziwei , MA Qianqian . Effects of Vegetable Carbon Black on Growth Performance and Reproductive System of Rats Fed Zearalenone Contaminated Diet[J]. Chinese Journal of Animal Nutrition, 2021 , 33(10) : 5827 -5837 . DOI: 10.3969/j.issn.1006-267x.2021.10.042

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