EXPERIMENTAL METHOD AND ANIMAL

Optimization of Extraction of Total Flavonoids from Total Mixed Ration of Dairy Cows by Response Surface Methodology

  • XIONG Anran ,
  • PAN Yucong ,
  • YU Shiqiang ,
  • JIANG Linshu ,
  • XIONG Benhai
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  • 1. Key Laboratory for Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    2. Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2021-03-30

  Online published: 2021-10-16

Supported by

 

Abstract

The purpose of this experiment was to explore the content of total flavonoids in total mixed ration (TMR) diet of dairy cows and the best ethanol extraction conditions, so as to provide technical support and theoretical basis for further study on the efficacy of nutritional active substances in feed and optimization of feed formula. In this experiment, TMR of high-yield lactating cows with an annual output of more than 12 t was selected as the sample, and the extraction amount of total flavonoids was taken as the investigation index, and ethanol was used as the extraction solvent. Response surface methodology (RSM) was used to explore the extraction effect of total flavonoids from TMR of dairy cows under different extraction conditions. The results showed that under the condition of water bath, the maximum extraction amount of total flavonoids was 9.15 mg/g when 1.0 g of air-dried ground feed was extracted with 80% ethanol (30 mL) at 80℃ for 80 min, while the maximum extraction amount was 10.97 mg/g when 90% ethanol (40 mL) was used for ultrasonic extraction for 40 min. Therefore, the content of total flavonoids extract from TMR by ultrasonic extraction is higher than that by water bath extraction, which provide a certain reference for the extraction of total flavonoids from TMR of dairy cows.

Cite this article

XIONG Anran , PAN Yucong , YU Shiqiang , JIANG Linshu , XIONG Benhai . Optimization of Extraction of Total Flavonoids from Total Mixed Ration of Dairy Cows by Response Surface Methodology[J]. Chinese Journal of Animal Nutrition, 2021 , 33(10) : 5849 -5860 . DOI: 10.3969/j.issn.1006-267x.2021.10.044

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