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Effects of Dietary Protease on Growth Performance, Glucose and Lipid Metabolism of Largemouth Bass (Micropterus salmoides)

  • GUAN Ying ,
  • XUE Jipeng ,
  • XUE Min ,
  • XIE Xiaoze ,
  • WU Xiufeng ,
  • ZHENG Yinhua ,
  • LIANG Xiaofang
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  • 1. The National Aquatic Feed Safety Assessment Center, Feed Research Institue, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. Key Laboratory of Applied Biology and Aquaculture Northern Fish in Liaoning Province, Dalian Ocean University, Dalian 116023, China;
    3. Guangzhou Bosio Biochemical Technology Research Co., Ltd., Guangzhou 511356, China

Received date: 2021-03-16

  Online published: 2021-10-16

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Abstract

The objectives of the study were to investigate the effects of dietary protease (NP) on growth performance, glucose and lipid metabolism of largemouth bass. A total of 240 Juvenile largemouth bass with an initial body weight of (31.39±0.05) g were randomly divided into two control groups and two experimental groups, with three replicates in each group and 20 fish in each replicate. The diet containing 50% fish meal (HFM group) was used as a positive control. The basal control diet (LFM group) contained 30% fish meal and 40% fish meal protein replaced by cottonseed protein concentrate (CPC). The experimental group was fed LFM group diet with 4 500 (LFM+E4500 group) and 7 500 U/kg NP (LFM+E7500 group). The essential amino acids and essential fatty acids were balanced in each group, and the feeding period was 65 days. The results showed as follows:1) compared with HFM group, the level of basal energy metabolism in CPC group was significantly lower than that in HFM group (P<0.05). 2) The survival rate was up-regulated to a certain extent (from 92.50% in LFM group to 98.33% in LFM+E4500 group and 100.00% in LFM+E7500 group) with protease adding. Compared with the LFM group, the abdominal fat rate of the experimental group decreased significantly (P<0.05), and the edible part of the fish increased, which greatly improved the commercial value and economic benefits of largemouth bass. 3) Compared with LFM group, the protease content in the intestine of the experimental group was significantly increased (P<0.05), so as to improve the digestion and absorption of protein. The activities of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) in liver were increased by adding 4 500 U/kg protease, which was helpful to the normal expression of gluconeogenesis related enzymes in fasting state and maintain the relative constant blood glucose content in a hungry state. The increased cyclic adenosine monophosphate (cAMP) content in the liver upregulated the mRNA relative expression of cAMP-response element binding protein (CREB),thus, the activities of the enzyme in liver lipopolysis process[adipose triglyceride lipase (ATGL) and monoacylglycerol lipase (MGL)] and abdominal lipolysis process[ATGL and hormone-sensitive lipase (HSL)] and mRNA relative expression levels were significantly up-regulated (P<0.05). In conclusion, adding 4 500 U/kg protease to low fish meal basal diet can further improve the basal energy metabolism level and the protease content in foregut and hindgut of largemouth bass, promote the normal expression of key enzymes of glucose metabolism in fasting state, and reduce the lipid accumulation in liver and fish body. However, excessive addition protease (7 500 U/kg) can reduce the protein deposition rate, improve the feed coefficient and reduce the feed utilization rate. It is suggested that 4 500 U/kg protease should be added to the diet of largemouth bass.

Cite this article

GUAN Ying , XUE Jipeng , XUE Min , XIE Xiaoze , WU Xiufeng , ZHENG Yinhua , LIANG Xiaofang . Effects of Dietary Protease on Growth Performance, Glucose and Lipid Metabolism of Largemouth Bass (Micropterus salmoides)[J]. Chinese Journal of Animal Nutrition, 2021 , 33(10) : 5974 -5988 . DOI: 10.3969/j.issn.1006-267x.2021.10.056

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